... the temperature sensitivity and chromosome loss phenotype of a well-characterized Aurora B mutant ( ip1-2 ). The uba1-W928R mutation increases histone H3-S10 phosphorylation in the ipl1-2 strain, indicating that uba1-W928R acts by increasing Ipl1 activity and/or reducing the opposing protein phosphatase 1 (PP1...

...Claudia Winkler; Raphael Rouget; Dan Wu; Monique Beullens; Aleyde Van Eynde; Mathieu Bollen ABSTRACT The ubiquitously expressed nuclear protein NIPP1 (also known as PPP1R8) recruits phosphoproteins for regulated dephosphorylation by the associated protein phosphatase PP1. To bypass the PP1...

..., under the master control of Cdk1. During entry into mitosis, Cdk1, in collaboration with MASTL kinase, represses the activity of the major mitotic protein phosphatases, PP1 and PP2A, thereby ensuring mitotic substrates remain phosphorylated. For cells to complete and exit mitosis, these phosphorylation...

... phosphorylation of mitotic proteins. Upon mitotic exit, cyclin-B–Cdk1 and Gwl kinases are inactivated, and mitotic substrates are dephosphorylated. Here, we have identified protein phosphatase-1 (PP1) as the phosphatase involved in the dephosphorylation of the activating site (Ser875) of Gwl. Depletion of PP1...

... to mark cell poles for growth, but how this complex activates Cdc42 is unknown. Here, we show that ectopic targeting of Tea4 to cell sides promotes the local activation of Cdc42 and cell growth. This activity requires that Tea4 binds the type I phosphatase (PP1) catalytic subunit Dis2 or Sds21...

...), a newly identified inhibitory subunit of protein phosphatase 1 (PP1), inhibited neurite growth in primary sensory neurons as an intrinsic regulator. IPP5 was highly expressed in the primary sensory neurons of rat dorsal root ganglion (DRG) and was downregulated after sciatic nerve axotomy. Knocking down...

...Maria Wiezlak; Jessica Diring; Jasmine Abella; Stephane Mouilleron; Michael Way; Neil Q. McDonald; Richard Treisman Summary The Phactr family of PP1-binding proteins is implicated in human diseases including Parkinson’s, cancer and myocardial infarction. Each Phactr protein contains four G-actin...

... understanding of phosphatase structure, and the importance of phosphatase function in the control of mitotic spindle formation, chromosome architecture and cohesion, and cell division. * Author for correspondence ( [email protected] ) © 2011. 2011 Cdk1 Kinase Mitosis PP1 PP2A...

...Isabel Alvarez-Tabarés; Agnes Grallert; Jose-Miguel Ortiz; Iain M. Hagan PP1 holoenzymes are composed of a small number of catalytic subunits and an array of regulatory, targeting, subunits. The Schizosaccharomyces pombe genome encodes two highly related catalytic subunits, Dis2 and Sds21...

... that PP2A depletion is sufficient to lead to Cdc2 activation, whereas Aurora-A activation depends on Cdc2 activity. The activity level of PP1 does not affect Cdc2 kinase activation promoted by PP2A removal. PP1 inhibition is also not sufficient to lead to Aurora-A activation in the absence of active Cdc2...

...Rikke L. Steen; Monique Beullens; Helga B. Landsverk; Mathieu Bollen; Philippe Collas Reassembly of the nuclear envelope (NE) at the end of mitosis requires targeting of the B-type lamin protein phosphatase, PP1, to the envelope by A-kinase anchoring protein AKAP149. We show here that NE-associated...

...Patricia T. W. Cohen Protein phosphatase 1 (PP1) is a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits. Most...

...Laura Trinkle-Mulcahy; Judith E. Sleeman; Angus I. Lamond Protein phosphatase 1 (PP1) is expressed in mammalian cells as three closely related isoforms, α, β/δ and γ1, which are encoded by separate genes. It has yet to be determined whether the separate isoforms behave in a similar fashion or play...

... is anchored in the axoneme. Moreover, analysis of flagella and axonemes from mutant strains revealed that PP1c is primarily, but not exclusively, anchored in the central pair apparatus, associated with the C1 microtubule. Thus, PP1 is part of the central pair mechanism thatcontrols flagellar motility. Two...

... of a novel gene product Hcn1, similar to budding yeast Cdc26. Furthermore, deletion of protein kinase PKA (Pka1) suppresses the phenotype of the cut9 mutation and reduces phosphorylation of Cut9. In contrast, PP1 (Dis2) phosphatase mutation shows the reverse effect on the phenotype of cut9 . The Cut9 subunit...