... mechanisms responsible for the remodeling of constitutive heterochromatin are largely unknown. Here, we identify that DAXX, an H3.3 chaperone essential for the maintenance of mouse ESCs in the ground state, accumulates in pericentromeric regions independently of DNA methylation. DAXX recruits PML and SETDB1...

... is a critical mechanism for silencing repetitive DNA. DAXX and ATRX are also components of promyelocytic nuclear bodies (PML-NBs), which have been identified as sites of H3.3 chromatin assembly. Here, we use a transgene array that can be visualized in single living cells to investigate the mechanisms...

... and have a poor prognosis. They are also commonly deficient for expression of ATRX protein, a repressor of ALT activity, and a component of promyelocytic leukemia nuclear bodies (PML NBs) that are required for intrinsic immunity to various viruses. Here, we asked whether ATRX deficiency creates...

...Chuang Li; Qiongfang Peng; Xiao Wan; Haili Sun; Jun Tang ABSTRACT Promyelocytic leukemia protein (PML) nuclear bodies (NBs), which are sub-nuclear protein structures, are involved in a variety of important cellular functions. PML-NBs are assembled by PML isoforms, and contact between small...

...Katherine J. Hands; Delphine Cuchet-Lourenco; Roger D. Everett; Ronald T. Hay SUMMARY Arsenic is a clinically effective treatment for acute promyelocytic leukaemia (APL) in which the promyelocytic leukaemia (PML) protein is fused to retinoic receptor alpha (RARα). PML-RARα is degraded...

... bodies (PML-NBs, also known as ND10) contribute to host restriction of herpes simplex virus type 1 (HSV-1) replication via mechanisms that are counteracted by viral regulatory protein ICP0. ND10 assembly is dependent on PML, which comprises several different isoforms, and depletion of all PML isoforms...

... to promyelocytic leukemia (PML) nuclear bodies through its interaction with PML-IV. Treatment of interferon-α (IFNα) in H1299 cells resulted in the increase of PML proteins with a concurrent decrease of telomerase activity, as previously reported. PML depletion, however, stimulated telomerase activity that had...

...Åsne Jul-Larsen; Amra Grudic; Rolf Bjerkvig; Stig Ove Bøe Nucleoporins and the promyelocytic leukemia protein (PML) represent structural entities of nuclear pore complexes and PML nuclear bodies, respectively. In addition, these proteins might function in a common biological mechanism, because...

... for correspondence (e-mail: [email protected] ) 16 9 2008 © The Company of Biologists Limited 2008 2008 SUMO RanGAP1 PML The capacity for members of the structurally related ubiquitin-like (Ubl) family, including SUMO, to profoundly influence the stability, subcellular localization...

...Wilfried Condemine; Yuki Takahashi; Morgane Le Bras; Hugues de Thé The promyelocytic leukemia (PML) tumour suppressor is the organiser of PML nuclear bodies, which are domains the precise functions of which are still disputed. We show that upon several types of stress, endogenous PML proteins form...

...Stig Ove Bøe; Marte Haave; Åsne Jul-Larsen; Amra Grudic; Rolf Bjerkvig; Per Eystein Lønning The promyelocytic leukemia protein (PML) participates in several cellular functions, including transcriptional regulation, apoptosis and maintenance of genomic stability. A key feature of this protein...

... binding protein (CBP) and acetylated histone H4, which are markers for transcriptionally active chromatin. Moreover, STAT3 nuclear bodies in HepG2 cells are not colocalized with promyelocytic leukemia oncoprotein (PML)-containing bodies; neither is a sumoylation of activated STAT3 detectable. Taken...

...Sari Kurki; Leena Latonen; Marikki Laiho Mdm2 is a nucleoplasmic and nucleolar protein interacting with p53 and alternative reading frame (ARF) tumor suppressor proteins. Here we demonstrate relocalization and novel interactions of Mdm2 with the promyelocytic leukemia (PML) protein following...

... constructs, we examined the intracellular localization of human HP1 subtypes during the cell cycle. During interphase, all three HP1 subtypes were localized to centromeric heterochromatin and to promyelocytic leukemia(PML) nuclear bodies. Different preferences, however, were observed among the subtypes...

... with promyelocytic leukemia (PML) protein in nuclear bodies implicated in transcription control. Since Drosophila ten-m acts epistatically to another pair-rule gene opa , we investigated whether gene regulation by the mammalian opa homologue zic-1 was influenced by the intracellular domain of teneurin-2. We found...

...Isabelle Nefkens; Dmitri G. Negorev; Alexander M. Ishov; Jennifer S. Michaelson; Edward T. H. Yeh; Robert M. Tanguay; Werner E. G. Müller; Gerd G. Maul Nuclear domains called ND10 or PML bodies might function as nuclear depots by recruiting or releasing certain proteins. Although recruitment...

... of nuclear PA28 is concentrated at promyelocytic leukemia oncoprotein (PML)-containing bodies also commonly known as PODs or ND10. IFNγ treatment greatly increased the number and size of the PA28- and PML-containing bodies, and the effect was further enhanced in serum- deprived cells. PML bodies...

...Dmitri Negorev; Alexander M. Ishov; Gerd G. Maul ABSTRACT Nuclear domains called ND10 or PML nuclear bodies consist of an aggregation of several proteins, most notably PML and Sp100. PML is essential in the nucleation and formation of ND10 as well as in the recruitment of other ND10-associated...

...Roger D. Everett; Patrick Lomonte; Thomas Sternsdorf; Roel van Driel; Anne Orr ABSTRACT The nuclear sub-structures known as ND10, PODs or PML nuclear bodies can be rapidly modified by diverse stimuli, and the resultant structural changes correlate with events such as cellular transformation...

...Estelle Duprez; Andrew J. Saurin; Joana M. Desterro; Valerie Lallemand-Breitenbach; Kathy Howe; Michael N. Boddy; Ellen Solomon; Hugues de Thé; Ronald T. Hay; Paul S. Freemont ABSTRACT PML is a nuclear phosphoprotein that was first identified as part of a translocated chromosomal fusion product...