... with respect to PINK1 +/+ MEFs and hPINK1 res MEFs, ### P <0.0001 with respect to PINK1 −/− MEFs (one-way ANOVA). Fig. 5. PIP3 localisation is PINK1 kinase-dependent. (A) Representative confocal images showing PIP3 localisation in PINK1+/+, PINK1−/−, hPINK1res and hPINK1TKD MEFs. Serum-starved cells...

... apart. Scale bar: 10 µm. Fig. 1. Stimulation with cAMP-coated micro-particles. An impaled Sepharose micro-particle that is exposing covalently bound cAMP molecules (6-AH-cAMP) on its surface was brought into contact with a differentiated Dictyostelium cell (D.d.) expressing the PIP3 marker PHCRAC–GFP...

... that is recruited, in a sustained manner, to the plasma membrane and thereby negatively regulates ARF6-mediated cytoskeleton actin reorganisation. Since centaurin-α 2 is structurally related to PIP3 binding centaurin-α 1 , we hypothesised that centaurin-α 2 may bind to PIP 3 or other PI 3-kinase lipid...

.... Localization and wortmannin-sensitive translocation of expressed PH-GFP chimeras that recognize PIP3 in NIH 3T3 cells. NIH 3T3 cells were transfected with the indicated PH-GFP constructs and live cells were examined by confocal microscopy after 1 day of transfection. Note the recruitment of the PH domains...