... bundle architecture is coupled to different F-actin affinities. This concept of structural bundle conversion appears to be the overarching theme of the vinculin–talin–F-actin association ( Fig. 6 D). In addition to tension-mediated h1 release, PIP2 interference has also been proposed to switch...

.... , Yamaguchi , H. , Shiraishi , M. , Shiraishi , M. , Fukami , K. , Tanabe , A. , Ikeda-Matsuo , Y. , Ikeda Matsuo , Y. , Naito , Y. , Naito , Y. , et al. ( 2009 ). MARCKS regulates lamellipodia formation induced by IGF-I via association with PIP2 and beta-actin...

.... Diverse membrane-associated molecules co-label common membrane structures. (A) Schematic of C. elegans zygote polarization, highlighting PAR-3 clusters, contractile foci and putative PIP2-enriched membrane domains. Polarization of PAR proteins (red–blue) is induced by anterior-directed actomyosin cortical...

... distinct effects on kinetics of depletion and recovery attributable to GMR-Gal4 expression, emphasising the need for GMR - Gal4 controls for UAS-RNAi experiments. (I) The canonical phosphoinositide cycle with identified and candidate genes indicated. Fig. 1. PIP2 and PI4P recovery time courses...

... - 925 . 10.1113/jphysiol.2007.132498 Chakrabarti , P. , Kolay , S. , Yadav , S. , Kumari , K. , Nair , A. , Trivedi , D. and Raghu , P. ( 2015 ). A dPIP5K dependent pool of phosphatidylinositol 4,5 bisphosphate (PIP2) is required for G-protein coupled signal...

...’– to sequester CaM (green). (C) Averaged current reduction after activation of DrVSP for the indicated Kv7 configurations, in resting conditions and with elevated (red) or reduced (green) CaM. Fig. 6. Disruption of coiled-coil formation affects calmodulin-dependent regulation of PIP2 sensitivity. (A) Scheme...

... of the confined cAMP stimulus and of the signal transduction pathway. (A) Schematic view of the spatial distribution of filamentous actin, PIP2, PIP3, PTEN and PHCRAC during extracellular cAMP stimulation of a confined membrane region. (B) Schematic view of the signaling pathway initiated by ligand binding...

... 4,5-bisphosphate (PIP2) is a part of the protein complex on the active ribosomal promoter during transcription. PIP2 makes a complex with Pol I and the Pol I transcription factor UBF in the nucleolus. PIP2 depletion reduces Pol I transcription, which can be rescued by the addition of exogenous PIP2...

... of syntenin with syndecan heparan sulphate proteoglycans, which act as co-receptors for adhesion molecules and growth factors. Furthermore, we show that the interaction of syntenin with phosphatidylinositol 4,5-bisphosphate (PIP2) and with the small GTPase ADP-ribosylation factor 6 (Arf6), which regulate...

... PIP2 © 2010. 2010 16 6 2010 § Author for correspondence ( [email protected] ) ‡ Present address: The CRUK Inositide Laboratory, The Paterson Institute for Cancer Research, Wilmslow Road, Manchester M20 4BX, UK * These authors contributed equally to this work...

.... Fig. 5. Recruitment of PIP 5-kinase at the sites of bacterial entry and local PIP2 production. (A) PIP 5-kinase localizes at the sites of bacteria entry. HeLa cells were transfected with HA-tagged PIP 5-kinase for 18 hours and infected with FITC-coupled Chlamydia for 5 minutes. Transfected cells were...

... (middle), or with an antibody to VSV and phalloidin (bottom) as shown. (Inset) Enlargement of box showing differences in actin coating and PIP2 accumulation in Gas3/PMP22 vacuoles. Bar, 3 μm. To characterize vacuoles positive to Gas3/PMP22, but negative for PIP 2 and actin, cells were labelled...