...-licence-1-1/ Highlighted Article: Circular dorsal ruffles in fibroblasts amplify PI3K-dependent signals leading to phosphorylation of Akt in response to EGF or low concentrations of PDGF. MTORC1 Macropinocytosis Microtubules PI 3-kinase Akt National Institutes of Health...

... interaction in cells with the active form of Raf-1 and with p110α, the catalytic subunit of PI 3-kinase. Because the majority of phosphorylated K-Ras is located at the plasma membrane, different localization within this membrane according to the phosphorylation status was explored. Density-gradient...

... ErbB2 and EGFR and abundant PI 3-kinase. Other docking proteins, such as Shc and STAT5, respond differently to receptor activation. Levels of Shc at the membrane increase two- to five-fold with EGF, whereas pre-associated STAT5 becomes strongly phosphorylated. These data suggest that the distinct...

... of PI 3-kinase-regulated Arf signaling. Trends Biochem. Sci. 25 , 489 -495. Jaffe, A. and Hall, A. ( 2005 ). Rho GTPases: biochemistry and biology. Annu. Rev. Cell Dev. Biol. 21 , 247 -269. Kaech, S., Parmar, H., Roelandse, M., Bornmann, C. and Matus, A. ( 2001 ). Cytoskeletal...

... requires p38 and activation of NFκB requires IκB kinase-β. Activation of β-catenin, important in Wnt signaling and the cause of most colon cancers, is inhibited by shear stress through a pathway involving laminin-5, α6β4 integrin, phosphoinositide 3-kinase (PI 3-kinase) and Rac1 coupled with changes...

... characterisation of centaurin-α 2 , which is structurally related to the centaurin-α 1 ARF6 GAP. centaurin-α 2 contains an N-terminal GAP domain followed by two pleckstrin homology (PH) domains (N-PH and C-PH). In vitro, GFP-centaurin-α 2 specifically binds the phosphatidylinositol (PI) 3-kinase lipid products, PI...

...Karine Lindmo; Harald Stenmark Phosphoinositide (PI) 3-kinases control essential cellular functions such as cytoskeletal dynamics, signal transduction and membrane trafficking. FYVE, PX and PH domains mediate the binding of effector proteins to the lipid products of PI 3-kinases. Recent studies...

.... Microbiol. Immunol. 282 , 49 -88. Falasca, M., Logan, S. K., Lehto, V. P., Baccante, G., Lemmon, M. A. and Schlessinger, J. ( 1998 ). Activation of phospholipase Cγ by PI 3-kinase-induced PH domain-mediated membrane targeting. EMBO J. 17 , 414 -422. Harlan, J. E., Hajduk, P. J., Yoon, H. S...

... 1 and ARAP3 (ARF GAP, Rho GAP, ankyrin repeat, PH protein 3) as PtdIns(3,4,5) P 3 binding proteins ( Hammonds-Odie et al., 1996 ; Krugmann et al., 2002 ). PtdIns(3,4,5) P 3 is a ubiquitous lipid second messenger produced by agonist-stimulated phosphoinositide (PI) 3-kinase and plays...

... activation of phosphoinositide 3-kinase (PI 3-kinase), phospholipase C (PLC) and phospholipase D (PLD). We therefore reexamined whether motility is constitutively triggered by v-Src and studied whether this response is controlled by the same signalling pathway. The study was performed using Rat-1/tsLA29...

... of the induced genes was Akt we investigated whether Akt played a role in terminal differentiation. Activation of PI 3-kinase but not Akt was necessary for FOXN1-induced differentiation. In reconstituted epidermis FOXN1 promoted early stages of terminal differentiation whereas Akt activation was sufficient...

... actin patches were larger than in non-transfected cells (arrowheads). Upstream as well as downstream effectors of Rho GTPases are the phosphatidylinositide (PI) 3-kinases, which catalyse D3-phosphorylation of phosphoinositides and are involved in several endocytic processes, including several...

...Keqiang Ye; Solomon H. Snyder Phosphoinositide (PI) 3-kinase enhancer (PIKE) is a brain-specific GTPase that binds to PI 3-kinase and stimulates its lipid kinase activity. It exists in two forms: the first to be identified, PIKE-S, is shorter and exclusively nuclear; by contrast, the longer form...

... the extension of cadherin contacts through a phosphoinositide 3-kinase (PI 3-kinase)-Rac1 pathway. Cadherin adhesions were formed by PI 3-kinase-independent, Rac1-dependent co-recruitment of adhesion complexes and actin filaments. The expression and localization of p120 at the plasma membrane, associated...

.... * Author for correspondence (e-mail: [email protected] ) © The Company of Biologists Limited 2003 2003 Chemotaxis Dictyostelium PI 3-kinase Fibroblasts Neutrophils Actin Chemotaxis, movement of a cell towards a chemoattractant signal, is a fundamental process involved in a wide...

... reduced actin polymerization and filopodia formation. Phospholipid imbalance was accompanied by a reversible translocation of phosphoinositide 3-kinase from cytoplasm to plasma membrane. In agreement with a role for PI 3-kinase, when phospholipids were added to platelets, PtdIns(3,4) P 2 increased two...

... PLC-gamma1 downstream from PI 3-kinase. Cell Microbiol. 2 , 465 -477. Bierne, H., Gouin, E., Roux, P., Caroni, P., Yin, H. L. and Cossart, P. ( 2001 ). A role for cofilin and LIM kinase in Listeria-induced phagocytosis. J. Cell Biol. 155 , 101 -112. Birchmeier, C. and Gherardi, E...

.... Ang2 showed no effect on proliferation, but stimulated chemotaxis and tube-like structure formation. Phosphoinositide 3-kinase (PI 3-kinase) was activated by Ang2 through c-Fes and was involved in chemotaxis toward Ang2. Ang2 also activated c-Fyn in IBE cells. Cells expressing kinase-inactive c-Fyn...

... Phosphoinositide PI 3-kinase Signal transduction Zinc finger Many biochemical processes in the cell occur at the interface between the cytosol and an intracellular membrane. Vesicular trafficking, signal transduction and actin-regulated membrane rearrangements are examples of processes that require...

... of plasma membrane PIP 3 production. Furthermore, the translocation requires the ability of either agonist to activate PI 3-kinase, since it is inhibited by wortmannin (100 nM), LY294002 (50 μM) and by coexpression with a dominant negative p85. This data therefore suggests that in vivo cytohesin-1 can...