... in VPS13A function. The pleckstrin homology (PH) domain at the C-terminal region of VPS13A is required to form a complex with the XK scramblase and for the co-localization of VPS13A with XK within the cell. Alphafold modeling was used to predict an interaction surface between VPS13A and XK. Mutations...

...–FAK binding. A mutation in the Rgnef pleckstrin homology (PH) domain inhibits adhesion formation, FAK localization, and FAK-Y397 and paxillin-Y118 phosphorylation without disrupting the Rgnef–FAK interaction. A GEF-inactive Rgnef mutant rescues FAK-Y397 phosphorylation and early adhesion localization...

... Endocytic internalization PH domain PI(4,5)P 2 and phosphatidylserine (PS) mainly localize to the cytosolic leaflet of the plasma membrane. The head groups of both PI(4,5)P 2 and PS are negatively charged, so their predominance in the cytosolic leaflet results in a net negative charge...

... is essential for linking the positional cues for the cleavage site to contractile-ring formation. However, how Mid1 domains cooperate to regulate cytokinesis is poorly understood. Here we unravel the functions of different Mid1 domains (motifs) by a series of truncations. We report that the conserved PH domain...

... binding to the Myo10-PH2 domain is involved in Myo10 trafficking and regulation of filopodia dynamics. * These authors contributed equally to this work ‡ Author for correspondence ( [email protected] ) 14 7 2010 © 2010. 2010 Endosome Filopodia Myosin-X PH domain...

...Péter Várnai; Tzvetanka Bondeva; Péter Tamás; Balázs Tóth; László Buday; László Hunyady; Tamas Balla Several pleckstrin-homology (PH) domains with the ability to bind phosphatidylinositol (3,4,5)-trisphosphate [PtdIns(3,4,5) P 3 , PIP 3 ] were expressed as green fluorescent protein (GFP...

... to overlap with a true PH domain, as revealed by the crystal structure of MTMR2 ( Begley et al., 2003 ). One major challenge is to understand the function of this domain and to account for how mutations in this domain may lead to inherited diseases. Our previous data and those of others have shown that point...

... ] and phosphatidylinositol 3,4-bisphosphate [PtdIns(3,4) P 2 ]. We used a panel of pleckstrin homology (PH) domains with distinct binding specificities for PtdIns(3,4,5) P 3 and PtdIns(3,4) P 2 to study the spatiotemporal dynamics of these phosphoinositides in vivo . During phagocytosis and macropinocytosis PtdIns(3,4,5) P...

... for the actual motility of PGCs. * Author for correspondence (e-mail: [email protected] ) 15 6 2004 © The Company of Biologists Limited 2004 2004 Danio rerio PGC Chemotaxis Cell migration PH domain G protein PI3K SDF-1 CXCR4 Chemokine Chemotaxis is defined as the ability...

... , and Morrison, 1994 , for reviews). We found that keratinocyte-derived stratifin harbors a pleckstrin homology (PH) domain and enhances protein kinase C activity. DEAE-cellulose was from Whatman (Maidston, UK). Sephadex G200, threonine-Sepharose 4B, Superdex 75 HR10/30, Mono Q HR 5/5 and ampholines...