... in VPS13A function. The pleckstrin homology (PH) domain at the C-terminal region of VPS13A is required to form a complex with the XK scramblase and for the co-localization of VPS13A with XK within the cell. Alphafold modeling was used to predict an interaction surface between VPS13A and XK. Mutations...

...–FAK binding. A mutation in the Rgnef pleckstrin homology (PH) domain inhibits adhesion formation, FAK localization, and FAK-Y397 and paxillin-Y118 phosphorylation without disrupting the Rgnef–FAK interaction. A GEF-inactive Rgnef mutant rescues FAK-Y397 phosphorylation and early adhesion localization...

... Endocytic internalization PH domain PI(4,5)P 2 and phosphatidylserine (PS) mainly localize to the cytosolic leaflet of the plasma membrane. The head groups of both PI(4,5)P 2 and PS are negatively charged, so their predominance in the cytosolic leaflet results in a net negative charge...

... is essential for linking the positional cues for the cleavage site to contractile-ring formation. However, how Mid1 domains cooperate to regulate cytokinesis is poorly understood. Here we unravel the functions of different Mid1 domains (motifs) by a series of truncations. We report that the conserved PH domain...

... binding to the Myo10-PH2 domain is involved in Myo10 trafficking and regulation of filopodia dynamics. * These authors contributed equally to this work ‡ Author for correspondence ( [email protected] ) 14 7 2010 © 2010. 2010 Endosome Filopodia Myosin-X PH domain...

...Péter Várnai; Tzvetanka Bondeva; Péter Tamás; Balázs Tóth; László Buday; László Hunyady; Tamas Balla Several pleckstrin-homology (PH) domains with the ability to bind phosphatidylinositol (3,4,5)-trisphosphate [PtdIns(3,4,5) P 3 , PIP 3 ] were expressed as green fluorescent protein (GFP...

... Myotubularin PH domain FYVE domain Phosphoinositides Phosphatase Golgi The myotubularins are a family of phosphoinositide 3-phosphatases that display distinct substrate specificity against PtdIns3 P and PtdIns(3,5) P 2 ( Blondeau et al., 2000 ; Schaletzky et al., 2003 ; Taylor et al., 2000...

... ] and phosphatidylinositol 3,4-bisphosphate [PtdIns(3,4) P 2 ]. We used a panel of pleckstrin homology (PH) domains with distinct binding specificities for PtdIns(3,4,5) P 3 and PtdIns(3,4) P 2 to study the spatiotemporal dynamics of these phosphoinositides in vivo . During phagocytosis and macropinocytosis PtdIns(3,4,5) P...

... for the actual motility of PGCs. * Author for correspondence (e-mail: [email protected] ) 15 6 2004 © The Company of Biologists Limited 2004 2004 Danio rerio PGC Chemotaxis Cell migration PH domain G protein PI3K SDF-1 CXCR4 Chemokine Chemotaxis is defined as the ability...

... , and Morrison, 1994 , for reviews). We found that keratinocyte-derived stratifin harbors a pleckstrin homology (PH) domain and enhances protein kinase C activity. DEAE-cellulose was from Whatman (Maidston, UK). Sephadex G200, threonine-Sepharose 4B, Superdex 75 HR10/30, Mono Q HR 5/5 and ampholines...