... for the assay. The indicated agonists [used at optimal concentrations (see supplementary material Fig. S1), antigen, Ag (10 ng/ml), SCF (10 ng/ml), adenosine (1 μM), PGE2 (100 nM)] were added in the lower chamber, and the migrated BMMCs were counted after 4 hours. Results are means ± s.e. of three separate...

.... and Sykes, M. ( 2005 ). Maturation of human monocyte-derived dendritic cells (MoDCs) in the presence of prostaglandin E2 optimizes CD4 and CD8 T cell-mediated responses to protein antigens: role of PGE2 in chemokine and cytokine expression by MoDCs. Int. Immunol. 17 , 1561 -1572. Scandella, E., Men...

...), sulprostone (1 μM) or PGE2 (1 μM) by elevation of cytosolic Ca2+ was determined. Control cells were left untreated. If indicated, the EP1 receptor antagonist SC19220 (10μM) was added 10 minutes prior to the experiment. The cytosolic Ca2+ concentration in untreated cells was about 50 nM; stimulation by AVP...