... cell fates and behaviours during development. To transduce canonical Wnt signals, Dishevelled binds to the intracellular face of Frizzled through its DEP domain and polymerises through its DIX domain to assemble dynamic signalosomes. Dishevelled also contains a PDZ domain, whose function remains...

... is essential, whereas its PDZ domain is dispensable, for signalling to β-catenin. Our results imply two mutually exclusive functions of the DEP domain in Wnt signal transduction – binding to Frizzled to recruit Dishevelled to the receptor complex, and dimerising to cross-link DIX domain polymers...

... resembles Ca 2+ -regulated exocytosis in neurons, we investigated whether cognate neuronal binding partners of the multi-PDZ domain protein MUPP1, which recruits molecules that control the initial tethering and/or docking between the acrosomal vesicle and the plasma membrane, are also expressed...

...Nina Stenzel; Christian P. Fetzer; Rolf Heumann; Kai S. Erdmann Multi-PDZ (PSD-95/Discs large/Zonula-occludens-1) domain proteins play a crucial role in the establishment and maintenance of cell polarization. The novel multi-PDZ domain protein FRMPD2 is a potential scaffolding protein consisting...

... components must be correctly positioned in both space and time. Adaptor molecules, which contain protein-interaction domains, are often involved in the assembly of multimeric complexes that organize intracellular signal-transduction pathways. One such protein is syntenin, a PDZ-domain-containing molecule...

... Leukocyte-endothelial cell interaction Tight junctions Cell polarity PDZ domain Cell-cell interactions are regulated by adhesion receptors on opposing cells. These receptors are frequently associated at the cytoplasmic surface with signalling molecules that respond to binding of extracellular...

... monolayers and disruption of polarity in MDCK cysts cultured in collagen. Both phenomena require the last four residues of CRB3. Next, we expressed, in MDCK cells, a dominant-negative Myc-Lin-2–Pals1 chimeric protein, where the PDZ domain of Lin-2 was replaced with that of Pals1. TJ and apical polarity...

... identified several proteins containing PDZ domains. One of these proteins, MAGI-3, contains a guanylate-kinase-like region, six PDZ and two WW domains. The interaction between RPTPβ and MAGI-3 was confirmed by co-immunoprecipitation and pulldown experiments in transfected cells. Immunofluorescence...

... Nitric oxide Heart Protein targeting PDZ domain The free radical gas nitric oxide (NO) is now recognized as a major messenger molecule that has diverse functions throughout the body. Although originally identified as the endothelial-derived relaxing factor( Furchgott and Zawadzki, 1980...

... insertion into the plasma membrane. Given that a number of studies have demonstrated the importance of PDZ-domain-containing proteins in ion channel trafficking, we set out to investigate how EBP50 influences TRPC4 localization and surface delivery in HEK293 cells. A mutant lacking the PDZ-interacting...

... 12 2000 © 2001 by Company of Biologists 2001 Apical polarization CFTR PDZ domain Localization signal Cystic fibrosis Achieving the correct cellular location of ion channels and transporters is essential for ion movement across epithelial cells. Elucidation of the mechanism...

... domain, a PDZ domain, a long proline-rich region, and a SAM domain. Binding partners for most of these domains have been identified: for instance, the PDZ domain of Shank proteins interacts with GKAP (a postsynaptic-density protein) as well as several G-protein-coupled receptors. The specific...

... to the epithelial lateral membrane. * Author for correspondence (E-mail: garner@nrc.uab.edu ) 15 06 1998 30 07 1998 © 1998 by Company of Biologists 1998 Actin Cell-cell contact Cytoskeleton MAGUK PDZ domain Members of the SAP97 family of synapse-associated proteins (SAPs...