... therapeutic uses for pharmacological inhibitors of PI3Kβ. NIH3T3 cells were seeded as described above and starved overnight in DMEM supplemented with 0.2% BSA and 0.5% FBS. PDGF-BB (0.5 nM) and 70 kDa TMR–dextran (1 mg/ml) were added to the cells for 30 min at 37°C. MDA-MB-231 cells were seeded on glass...

... and CrkL. We used gene silencing combined with mutational analysis to probe the role of Crk adaptors in platelet-derived growth-factor receptor β (PDGFβR) signaling. We demonstrate that Crk adaptors are required for formation of focal adhesions, and for PDGF-stimulated remodeling of the actin cytoskeleton...

... investigated the activation of Src or Fyn by tau, both in vitro and in vivo. Tau increased the kinase activity in in vitro assays and in transfected COS7 cells. In platelet-derived growth factor (PDGF)-stimulated fibroblasts, tau appeared to prime Src for activation following PDGF stimulation, as reflected...

... formation and decreased Rac activation following PDGF treatment. In addition, we describe a novel interaction between palladin and Eps8, a receptor tyrosine kinase (RTK) substrate that participates in the activation of the Rac-specific guanine nucleotide-exchange function of Sos-1. Eps8 was identified...

...Laurence Veracini; Mélanie Franco; Anthony Boureux; Valérie Simon; Serge Roche; Christine Benistant The mechanism by which the Src family of protein-tyrosine kinases (SFKs) regulate mitogenesis and morphological changes induced by platelet-derived growth factor (PDGF) is not well known...

... with platelet-derived growth factor (PDGF). The enzymatic activity of PLD1 was stimulated by adding PtdIns(3,4,5) P 3 in vitro. Treatment with PDGF resulted in the significant increase of PLD1 activity and phosphorylation of the downstream extracellular signal-regulated kinases (ERKs), which was blocked by pre...

...Mitsunobu R. Kano; Yasuyuki Morishita; Caname Iwata; Shigeru Iwasaka; Tetsuro Watabe; Yasuyoshi Ouchi; Kohei Miyazono; Keiji Miyazawa Combined stimulation with VEGF-A, FGF-2, or PDGF-BB has emerged as a potent strategy for therapeutic angiogenesis, although the mechanisms underlying the synergism...

... (PtdIns) 3-kinase, actin cytoskeletal remodeling and the v-SNARE VAMP2. The growth factor PDGF-BB also robustly activates PtdIns 3-kinase and induces actin remodeling, raising the question of whether it uses similar mechanisms to insulin in mobilizing GLUT4. In L6 myoblasts stably expressing Myc-tagged...

... molecule (PSA-NCAM) in the directional migration of OPCs towards platelet-derived growth factor (PDGF). Using a Boyden microchemotaxis chamber and the Dunn direct viewing chamber, we show that in concentration gradients of PDGF, PSA-positive OPCs polarize and efficiently migrate towards the source of PDGF...

...Inés M. Antón; Stephen P. Saville; Michael J. Byrne; Claudia Curcio; Narayanaswamy Ramesh; John H. Hartwig; Raif S. Geha Platelet-derived growth factor (PDGF) is a chemotactic factor for fibroblasts that triggers actin cytoskeleton reorganization by increasing the level of GTP-Rac, the activated...

... and expressed cartilage-specific genes in 7 to 16 day pellet cultures. Depositions of cartilage matrix and type II collagen were detected by day 14. TGFβ-stimulated chondrogenesis was synergistically enhanced by PDGF-BB,resulting in a larger cartilage particle filled with a cartilaginous area containing type II...

... of Biologists 2000 PI3-kinase Cytoskeleton PDGF Insulin Phosphoinositide 3´-kinase (PI3-K) activity induced by growth factor activation of several cell surface receptors leads to the production of the membrane lipids phosphatidylinositol-3-phosphate (PtdIns(3)P), PtdIns(3,4)P 2 and PtdIns...

... 1998 by Company of Biologists 1998 PDGF Actin Ruffle Tyrosine phosphorylation The platelet-derived growth factor (PDGF) is a dimeric protein composed of A- and B-chains, which combine to form three isoforms, PDGF-AA, -AB and -BB ( Heldin and Westermark, 1996 ). The α-granules...

... in the regulation of the latter process are unknown, we investigated possible modulating effects of the cytokines epidermal growth factor (EGF), platelet-derived growth factor (PDGF), interleukin-1α (IL-1α) and transforming growth factor-β (TGF-β) on both collagen phagocytosis and the release of collagenase...

... chemotaxis chamber visual chemotaxis assay neutrophil chemotaxis fibroblast chemotaxis cell orientation diffusion theory chemotactic gradient formation and decay Helber bacteria counting chamber FMLP PDGF The Boyden chamber has become established as the routine method for studying chemotaxis...

... oligodendrocytes. In this article we summarize evidence that platelet-derived growth factor (PDGF) is important for stimulating O-2A progenitor cell proliferation in vivo , and describe our efforts to map the distribution of PDGF and its receptors in the developing rat CNS by in situ hybridization...

...Robert F. Brooks; Mark Howard; David S. Leake; Peter N. Riddle ABSTRACT Platelet-derived growth factor (PDGF) has been reported to be a potent mitogen for Swiss 3T3 cells made quiescent by growth to saturation density in high serum, and its activity is further potentiated by high levels of insulin...

... but not in the 3Y1 cells. The inhibitory effect of the platelet lysate was neutralized by anti-TGF- β 1 IgG. On the other hand, PDGF added to the fresh medium had no effect on either type of cells. These results suggest that cultured fibroblasts are capable of proliferating on a plastic substratum under fluid...