... ‘closed’ and ‘open’ conformations by fluorescence resonance energy transfer analysis. Using this probe we resolve two distinct Syx conformational transitions during membrane depolarization-induced exocytosis in PC12 cells: a partial ‘opening’ in the absence of Ca 2+ entry and an additional ‘opening’ upon...

... in differentiated neurons and neurosecretory cells (neural cells). Its role in proliferation has been investigated so far only in rapidly growing tumors, with conflicting results: suppression in non-neural tumors, stimulation in medulloblastomas. Working with two clones of chromaffin–neuronal PC12 cells, which...

...), was investigated in undifferentiated neuroendocrine PC12 cells. Live cell imaging and 20°C block–release experiments showed that VAChT–GFP, SYN–GFP and CgA–RFP specifically and transiently cohabitated in a distinct sorting compartment during cold block and then separated into synaptic protein transport vesicles...

..., Milan, Italy ‡ Author for correspondence ( meldolesi.jacopo@hsr.it ) 3 11 2009 © 2010. 2010 Rac1 Nerve-cell differentiation Neuronal primary cultures PC12 cells SH-SY5Y cells Vamp4 The neurite outgrowth taking place in neurons (during in vivo differentiation and upon...

... and upstream molecular events that might integrate it to the exocytotic machinery. Using PC12 cells, we found that Rac1 is associated with the plasma membrane and is activated during exocytosis. Silencing of Rac1 by siRNA inhibits hormone release, prevents secretagogue (high K + )-evoked phospholipase D1 (PLD1...

... in PC12 cells. J. Cell Biol. 173 , 241 -251. Banerjee, A., Li, G., Alexander, E. A. and Schwartz, J. H. ( 2001 ). Role of SNAP-23 in trafficking of H + -ATPase in cultured inner medullary collecting duct cells. Am. J. Physiol., Cell Physiol. 280 , C775 -C781. Bao, Y., Lopez, J. A., James...

.... PC12 cells were used to determine whether Cdk5, a critical participant in both processes, regulates the action of Kalirin. Expression of Kalirin-7 in nondifferentiated PC12 cells caused GEF-activity-dependent extension of broad cytoplasmic protrusions; coexpression of dominant-negative Cdk5 largely...

... and constitutively active form of the ALK protein tyrosine kinase (PTK) domain induced the neuron-like differentiation of PC12 cells through specific activation of the mitogen-activated protein kinase (MAP kinase) pathway. Its PTK domain had been originally identified in a nucleo-cytosolic and constitutively active...

... of nerve growth factor (NGF). Here, we report that the cAMP-dependent protein kinase A (PKA) induced the phosphorylation of Src at residue serine17 (S17) in multiple cell types including PC12, Hek293, AtT-20 and CHO cells. In PC12 cells, Src phosphorylation on S17 participates in the activation...

... to lipid rafts and is enriched in growth cones of differentiating PC12 cells following growth factor stimulation. We found that Pyk2 and Cbl interacted with the adaptor protein ArgBP2, which also bound to flotillin-1, a component of lipid raft microdomains. These interactions contributed to recruitment...

... of glutamine to glutamate or to arginine in the central zero layer residues of SNAP-25 did not impair the extent, time course or Ca 2+ -dependency of exocytosis in PC12 cells. Using adrenal chromaffin cells, we found that exocytosis could be reconstituted in cells transfected to express BoNT/E. A double Q→E...

... Limited 1993 1993 synaptic vesicles endosome synaptobrevin VAMP PC12 cells Journal o f Cell Science, Supplem ent 17, 81-83 (1993) Printed in Great Britain © The Company o f Biologists Limited 1993 81 Biogenesis of synaptic vesicles R. B. Kelly, Frank Bonzelius, Ann Cleves, Lois Clift-O Grady...

... (in the medium and cells, respectively). β -Xyloside inhibition of proteoglycan biosynthesis was accompanied by significant morphological effects in NGF-treated PC12 cells, consisting of an increase in length and decrease in the branching, diameter and adhesion to the collagen substratum of the PC 12 cell...