...David M. Hollenstein; Rubén Gómez-Sánchez; Akif Ciftci; Franziska Kriegenburg; Muriel Mari; Raffaela Torggler; Mariya Licheva; Fulvio Reggiori; Claudine Kraft ABSTRACT Autophagy is initiated by the formation of a phagophore assembly site (PAS), the precursor of autophagosomes. In mammals...

..., septins polymerize into ring-shaped arrays of filaments required for cytokinesis. In an unbiased genetic screen and in subsequent targeted analysis, we found autophagy defects in septin mutants. Upon autophagy induction, pre-assembled septin complexes relocalized to the pre-autophagosomal structure (PAS...

... from the pre-autophagosomal structure (PAS); ultimately, the IM expands to become a mature autophagosome. Eighteen autophagy-related (Atg) proteins are engaged in autophagosome formation at the PAS. However, the cup-shaped IM was visualized just as a dot by fluorescence microscopy, posing a challenge...

...-terminal heterodimerization (PAS) domain of HIF-1α and identify Ser247 as a major site of in vitro modification by casein kinase 1δ (CK1δ). Mutation of this site to alanine, surprisingly, enhanced the transcriptional activity of HIF-1α, a result phenocopied by inhibition or small interfering RNA (siRNA...