...+ entry in many different cell types. The CRAC channel is composed of the plasma membrane (PM)-localized Orai1 channel and endoplasmic reticulum (ER)-localized STIM1 Ca 2+ sensor. Upon ER Ca 2+ store depletion, Orai1 and STIM1 form complexes at ER–PM junctions, leading to the formation of activated CRAC...

... Stim1 oligomerization and its activation of Orai. Ca 2+ GPN LMP Orai1 Stim1 Lysosome Membrane-permeant lysosomotropic agents have long been used to disrupt lysosomal function and include two distinct classes: peptides or cationic amphiphilic drugs. Peptides such as glycyl-L...

...+ -dependent inactivation of ORAI1 Channels . J. Biol. Chem.   284 , 24933 - 24938 . 10.1074/jbc.C109.024083 Fahrner , M. , Muik , M. , Schindl , R. , Butorac , C. , Stathopulos , P. , Zheng , L. , Jardin , I. , Ikura , M. and Romanin , C. ( 2014 ). A coiled...

...Dalia Alansary; Diana B. Peckys; Barbara A. Niemeyer; Niels de Jonge ABSTRACT ORAI1 proteins form highly selective Ca 2+ channels in the plasma membrane. Crystallographic data point towards a hexameric stoichiometry of ORAI1 channels, whereas optical methods postulated ORAI1 channels to reside...

...-401L and MEG-01 cells resulted in a reduction in STIM1 tyrosine phosphorylation, SOCE and the Ca 2+ release-activated Ca 2+ current ( I CRAC ). STIM1–Orai1 colocalization was reduced in HEK293 cells transfected with YFP–STIM1-Y316F compared to in cells with wild-type (WT) YFP-tagged STIM1. Additionally...

...Marek K. Korzeniowski; Eva Wisniewski; Barbara Baird; David A. Holowka; Tamas Balla ABSTRACT Decreased luminal endoplasmic reticulum (ER) Ca 2+ concentration triggers oligomerization and clustering of the ER Ca 2+ sensor STIM1 to promote its association with plasma membrane Orai1 Ca 2+ channels...

...+ -selective channel Orai1. Here, we quantitatively dissect Orai1 trafficking dynamics and show that Orai1 recycles rapidly at the plasma membrane ( K ex ≃0.1 min −1 ), with ∼40% of the total Orai1 pool localizing to the plasma membrane at steady state. A subset of intracellular Orai1 localizes to a sub...

... through intricate coordination between the Ca 2+ sensor on the ER membrane (stromal interaction molecule 1, STIM1) and the plasma membrane channel Orai1. When Ca 2+ stores are depleted, STIM1 oligomerizes and physically interacts with Orai1 through its SOAR/CAD domain, resulting in Orai1 gating and Ca 2...

... is downregulated during mouse meiosis, but remains active in mature metaphase II eggs. SOCE inhibition is due to a decreased ability of the Ca 2+ sensor STIM1 to translocate to the cortical endoplasmic reticulum domain and due to internalization of Orai1. Reversing SOCE downregulation by overexpression of STIM1...

...) in vivo and thus regulates fluid secretion. In Cav1 −/− mouse SG, agonist-stimulated Ca 2+ entry and fluid secretion are significantly reduced. Microdomain localization of TRPC1 and interaction with its regulatory protein, STIM1, are disrupted in Cav1 −/− SG acinar cells, whereas Orai1–STIM1 interaction...

...Mirko Völkers; Nima Dolatabadi; Natalie Gude; Patrick Most; Mark A. Sussman; David Hassel Mutations in the store-operated Ca 2+ entry pore protein ORAI1 have been reported to cause myopathies in human patients but the mechanism involved is not known. Cardiomyocytes express ORAI1 but its role...

... cells. In the present study, we find that association of the endoplasmic reticulum (ER) Ca 2+ sensor, STIM1, and the store-operated Ca 2+ channel, Orai1, stimulated by thapsigargin-mediated ER store depletion, is enhanced by overexpression of PIP5KIβ and inhibited by overexpression of PIP5KIγ...

... of store-operated channels. e-mail: [email protected] 19 4 2007 © The Company of Biologists Limited 2007 2007 Store-operated Ca 2+ entry Ca 2+ channels Stim1 Orai1 Ca 2+ -release-activated Ca 2+ current In a variety of cell types, activation of phospholipase C...