...Kara Stark; Olivia Crowe; Lindsay Lewellyn ABSTRACT Intercellular bridges are essential for fertility in many organisms. The developing fruit fly egg has become the premier model system to study intercellular bridges. During oogenesis, the oocyte is connected to supporting nurse cells by relatively...

... transport. crb mRNA enrichment in the oocyte is affected in egl mutant egg chambers. A CRISPR-based genomic deletion of the crb locus that includes the LE disrupts asymmetric crb mRNA localisation in epithelia and prevents its accumulation in the oocyte during early stages of oogenesis, but does not affect...

..., Drosophila Wash was found to be essential for oogenesis and larval development. Recently, however, Drosophila wash was reported to be homozygous viable. Here, we verify that the original wash null allele harbors an unrelated lethal background mutation; however, this unrelated lethal mutation does...

... step in localization of oskar mRNA. These signals are collectively strong but individually weak, a property essential for later steps of localization. mRNA localization mRNA transport Oskar Bruno TLS Oogenesis National Institutes of Health 10.13039/100000002 R01GM54409...

... 2015. Published by The Company of Biologists Ltd 2015 Summary: mRNA translational control is vital for gamete development. One of three eIF4Es expressed in C. elegans germ cells recruits mRNAs at critical junctures for both egg and sperm maturation. mRNA translational control Oogenesis...

... Nostrin E-cadherin Membrane trafficking Drosophila Cell polarity Wing epithelium Oogenesis Kinesins Microtubules Members of the Fes–CIP4 homology Bin–amphiphysin–Rvs161/167 (F-BAR) protein family form crescent-shaped dimers that are able to shape membranes into vesicles and tubules...

... ONN, UK ‡ These authors contributed equally to this work Author for correspondence ( [email protected] ) 6 3 2012 © 2012. Published by The Company of Biologists Ltd 2012 Oogenesis Drosophila Germ cells Cup Oskar In Drosophila , germ cell differentiation...

... affecting the Drosophila melanogaster follicular epithelium, we isolated a mutation in CoREST that disrupts the Notch-dependent mitotic-to-endocycle switch of follicle cells at stage 6 of oogenesis. We show that Drosophila CoREST positively regulates Notch signaling, acting downstream of the proteolytic...

... analysis of α- Cat mutants in embryogenesis, imaginal discs and oogenesis reveals defects consistent with a loss of cadherin function. Compromising components of the Arp2/3 complex or its regulator SCAR ameliorate the α- Cat loss-of-function phenotype in embryos but not in ovaries, suggesting negative...

... Drosophila oogenesis. This effect appears to be mediated by grk mRNA and its protein-binding partners Squid and Hrp48/Hrb27C, implicating the grk mRNA–protein (mRNP) complex as a rate-limiting host factor controlling Wolbachia titer. Furthermore, highly infected flies exhibit defects that match those...

...Melissa A. Henderson; Elizabeth Cronland; Steve Dunkelbarger; Vince Contreras; Susan Strome; Brett D. Keiper Fertility and embryonic viability are measures of efficient germ cell growth and development. During oogenesis and spermatogenesis, new proteins are required for both mitotic expansion...

... [PtdIns(4,5) P 2 ] are involved in the early steps of the clathrin-dependent endocytic pathway, but little is known about how their functions are coordinated. We have studied the role of PtdIns(4,5) P 2 and Rab5 in the Drosophila germline during oogenesis . We found that Rab5 is required...

...Angela M. Jaramillo; Timothy T. Weil; Joseph Goodhouse; Elizabeth R. Gavis; Trudi Schupbach During Drosophila oogenesis, the targeted localization of gurken ( grk ) mRNA leads to the establishment of the axis polarity of the egg. In early stages of oogenesis, grk mRNA is found at the posterior...

... by Gja4 ), whereas those between granulosa cells are assembled from connexin 43 (Cx43, encoded by Gja1 ). This spatial separation, and the different permeability properties of gap junctions composed of Cx37 and Cx43, suggests that Cx37 channels serve a unique function in oogenesis. Female mice lacking...

.... M. ( 2005 ). Differential contributions of connexin37 and connexin43 to oogenesis revealed in chimeric reaggregated mouse ovaries. J. Cell. Sci. 118 , 5071 -5078. Gittens, J. E., Mhawi, A. A., Lidington, D., Ouellette, Y. and Kidder, G. M. ( 2003 ). Functional analysis of gap junctions...

... by a full-length JIL-1 transgenic construct. Thus, our results suggest that the JIL-1 kinase is required to maintain nuclear morphology and integrity of nurse cells during oogenesis and that this function may be linked to molecular interactions with lamin Dm 0 . * Author for correspondence (e...

... cells that are formed from an arrested cleavage furrow by addition of several actin-binding proteins ( Mahowald, 1971 ; Robinson et al., 1997 ; Sokol and Cooley, 1999 ). At stage 10-11 of oogenesis, actin struts form `cages' around the nurse cell nuclei and are thought to hold these nuclei in place...

..., although membrane trafficking defects in these oocytes result in aberrant dorsal appendages ( Murthy and Schwarz, 2004 ). The overexpression of HA-Sec8, which had no deleterious effect on wild-type oogenesis, enhanced the phenotype of sec5 E13 alone by arresting egg-chamber development between stages 7...

.... Importantly, the quality control mechanisms that ensure genetic integrity of gametes during meiosis also show a striking sexual dimorphism ( Hunt and Hassold, 2002 ). It has been shown that the pachytene and spindle assembly checkpoints are missing or less stringent in mammalian oogenesis than...

... is delayed but not eliminated in these mutants. There may also be an interaction between the recruitment of repair proteins and phosphorylation. References Abdu, U., Brodsky, M. and Schupbach, T. ( 2002 ). Activation of a meiotic checkpoint during Drosophila oogenesis regulates the translation...