... adhesion and permeability barrier. An intriguing characteristic of a TJ is the dynamic nature of its multiprotein complex. Occludin is the most mobile TJ protein, but its significance in TJ dynamics is poorly understood. On the basis of phosphorylation sites, we distinguished a sequence in the C-terminal...

...Xin Cong; Yan Zhang; Ning-Yan Yang; Jing Li; Chong Ding; Qian-Wen Ding; Yun-Chao Su; Mei Mei; Xiao-Hong Guo; Li-Ling Wu; Guang-Yan Yu Summary Occludin plays an important role in maintaining tight junction barrier function in many types of epithelia. We previously reported that activation...

... between tissue compartments and consist of tight-junction-associated marvel proteins (TAMPs) and claudins. The function of TAMPs and the interaction with claudins are not understood. We therefore investigated the binding between the TAMPs occludin, tricellulin, and marvelD3 and their interaction...

..., quantification of occludin and claudin-1–claudin-5 and claudin-7 was performed by densitometric analysis of western blots with normalization to β-actin as a loading control. Protein expression of claudin-1 was strongly increased by addition of berberine ( Fig. 2A ; berberine 174±10% of controls, P <0.001, n...

...Christina M. Van Itallie; Alan S. Fanning; Jennifer Holmes; James M. Anderson The function of occludin remains elusive. Proposed roles include maintenance of tight junction barriers, signaling and junction remodeling. To investigate a potential role in mediating cytokine-induced changes in barrier...

... expression increases myosin light chain phosphorylation, reorganizes perijunctional F-actin, and increases tight junction permeability. TJ proteins ZO-1 and occludin are markedly redistributed, morphologically and biochemically, but effects on claudin-1 and claudin-2 are limited. tMLCK inhibition prevents...

... Limited 2004 2004 Rotavirus VP8 Tight junction Occludin Claudin Drug delivery Rotaviruses are a major cause of diarrhea in young mammals. In developing countries rotavirus infections cause 600,000 childhood deaths per year and constitute an important source of morbidity...

... of apoptosis in epithelial cells, tight junction proteins undergo proteolytic cleavage in a distinctive manner correlated with a disruption of tight junctions. The transmembrane protein occludin and, likewise, the cytoplasmic adaptor proteins ZO-1 and ZO-2 are fragmented by caspase cleavage. In addition...

...Junichi Ikenouchi; Miho Matsuda; Mikio Furuse; Shoichiro Tsukita Snail is a transcription repressor that plays a central role in the epithelium-mesenchyme transition (EMT), by which epithelial cells lose their polarity. Claudins and occludin are integral membrane proteins localized at tight...

... (<100 Ωcm 2 ). Genuine expression of claudin-1 and claudin-2, but not of occludin or claudin-3, was reciprocal to transepithelial resistance. However,confocal microscopy revealed a marked subjunctional localization of claudin-1 in C11 cells, indicating that claudin-1 is not functionally related...

... the transepithelial resistance and increased the paracellular flux of [ 3 H]inulin within 24 hours. Reduced levels of the tight junction proteins claudin-1 and occludin accompanied the loss of barrier function. N-cadherin, expressed only in few cells of untreated or single-stimulated cultures, was at the same time...

...M. Reza Ghassemifar; Bhavwanti Sheth; Tom Papenbrock; Henry J. Leese; Franchesca D. Houghton; Tom P. Fleming The tight junction protein occludin possesses four transmembrane domains,two extracellular loops, and cytoplasmic N- and C-termini. Reverse transcription-PCR analysis of human tissues...

...K. M. McCarthy; S. A. Francis; J. M. McCormack; J. Lai; R. A. Rogers; I. B. Skare; R. D. Lynch; E. E. Schneeberger ABSTRACT Occludin and 18 distinct members of the claudin family are tetra-span transmembrane proteins that are localized in cell-specific tight junctions (TJs). A previous study showed...

... in transepithelial electrical resistance. Occludin, the first identified transmembrane protein to be localized to the tight junction of both epithelial and endothelial cells is thought play an important role in tight junction barriers. Although transepithelial electrical resistance fell to less than 20% of initial...

...J. Mankertz; S. Tavalali; H. Schmitz; A. Mankertz; E. O. Riecken; M. Fromm; J. D. Schulzke ABSTRACT The 65 kDa protein occludin is a membrane-spanning part of the epithelial tight junction, which is the main barrier of the paracellular pathway. The function of occludin as part of tight junctions...

...A. Nusrat; C. A. Parkos; P. Verkade; C. S. Foley; T. W. Liang; W. Innis-Whitehouse; K. K. Eastburn; J. L. Madara ABSTRACT Tight junctions (TJ) of polarized epithelial cells regulate barrier function at mucosal surfaces. Structural proteins of TJs include hyperphosphorylated occludin (HO...

... that MDCK-W cells treated with ouabain increase tyrosine phosphorylation and content of active MAP kinase, redistribute molecules involved in cell attachment (occludin, ZO-1, desmoplakin, cytokeratin, α;-actinin, vinculin and actin), and detach. Genistein and UO126, inhibitors of protein tyrosine kinase...

... analyzed the impact of protein tyrosine phosphatase (PTP) inhibition on the structure of endothelial junctional proteins. In human umbilical vein endothelial cells (HUVECs) the PTP inhibitors phenylarsine oxide (PAO) and pervanadate induced proteolysis of the tight junction protein occludin. Occludin...

... been implicated in many other cellular functions, including cell signaling and growth regulation, but they have yet to be directly implicated in vesicle movement. Occludin is a transmembrane protein located at tight junctions and is known to interact with other tight junction proteins, including ZO-1...

...Simon D. Bamforth; Uwe Kniesel; Hartwig Wolburg; Britta Engelhardt; Werner Risau ABSTRACT The tight junction is the most apical intercellular junction of epithelial cells and forms a diffusion barrier between individual cells. Occludin is an integral membrane protein specifically associated...