... feedback between mechanical force and biochemical signals, including epigenomic modifications that establish transcriptional programs. These programs, in turn, reinforce the mechanical properties of the cell and its ability to withstand mechanical perturbation. The nucleus has long been hypothesized...

... acetylation, RNA polymerase II fates, and macromolecular complex packing. Compared to proliferating cells, G0 cells have lower levels of histone acetylation, nuclear RNA polymerase II, and active transcription. The G0 nucleus has similar macromolecular crowding yet fewer chromatin-associated multi-megadalton...

... Blucher; Jillian Hardy; Jason Chen; Schuyler Figueroa; Vadim Backman; Anne Janssen; Mary Packard; Katherine Dorfman; Luay Almassalha; Michael Seifu Bahiru; Andrew D. Stephens ABSTRACT Nuclear blebs are herniations of the nucleus that occur in diseased nuclei and cause nuclear rupture leading to cellular...

...Marta Gawor; Lilya Lehka; Danielle Lambert; Christopher P. Toseland ABSTRACT Over the past two decades, significant progress has been made in understanding mechanotransduction to the nucleus. Nevertheless, most research has focused on outside-in signalling orchestrated by external mechanical...

... revealed that the nucleolus is encapsulated by a single-stranded (ss)DNA-based molecular complex inside the cell nucleus. Super-resolution lattice-structured illumination microscopy (lattice-SIM) showed that there was a high abundance of ssDNA beyond the ‘outer shell’ of the nucleolus. Nucleolar disruption...

... researchers using tagged lamins to study the nucleus. * Author for correspondence ( [email protected] ) Handling Editor: Megan King Competing interests The authors declare no competing or financial interests. 25 4 2024 29 7 2024 © 2024. Published by The Company...

... it is now well recognized that such confined migration is limited by the nucleus, which is the largest and stiffest organelle, it remains incompletely understood how cells apply sufficient force to move their nucleus through small constrictions. Here, we report a mechanism by which contraction of the cell...

... of transcription suppress both nuclear bleb formation and stabilization without altering nuclear rigidity, actin compression or actin contraction, suggesting new biophysics for nuclear morphology. Chromatin Mechanobiology Nucleus Nuclear blebbing National Institutes of Health http://dx.doi.org...

... been postulated that the unique segmented nucleus of neutrophils functions to assist their rapid migration. Here, we tested this hypothesis by imaging primary human neutrophils traversing narrow channels in custom-designed microfluidic devices. Individuals were given an intravenous low dose...

..., the contribution of CDK to stationary phase remains largely uncharacterized. Here, we examine the role of the sole S. pombe CDK, Cdc2, in the establishment of stationary phase. We show that in stationary phase, nuclear and chromosomal volumes and the nucleus-to-cell volume ratio are reduced, and sister chromatid...

...Sandrine B. Lavenus; Karl W. Vosatka; Alexa P. Caruso; Maria F. Ullo; Ayesha Khan; Jeremy S. Logue ABSTRACT When metastasizing, tumor cells must traverse environments with diverse physicochemical properties. Recently, the cell nucleus has emerged as a major regulator of the transition from...

...Sanju Ashraf; Ye Dee Tay; David A. Kelly; Kenneth E. Sawin ABSTRACT Movement of the cell nucleus typically involves the cytoskeleton and either polymerization-based pushing forces or motor-based pulling forces. In the fission yeast Schizosaccharomyces pombe , nuclear movement and positioning...

... chondrocyte quiescence and immature chondrocyte state through reducing the amount of H3K9me3-marked heterochromatin. Chondrocyte Differentiation Heterochromatin Hydrostatic pressure Mechanotransduction Nucleus Replicative stress Helsinki Institute of Life Science Wihuri...

... of adenovirus (AdV) infection. AdV delivers, transcribes and replicates a linear double-stranded DNA genome in the nucleus. We measured the abundance of viral transcripts using single-molecule RNA fluorescence in situ hybridization (FISH) and the incoming 5-ethynyl-2′-deoxycytidine (EdC)-tagged viral genomes...

...Justin T. Jacob; Raji R. Nair; Brian G. Poll; Christopher M. Pineda; Ryan P. Hobbs; Michael J. Matunis; Pierre A. Coulombe ABSTRACT Keratin 17 ( KRT17 ; K17), a non-lamin intermediate filament protein, was recently found to occur in the nucleus. We report here on K17-dependent differences...

... work is properly attributed. Summary: Nuclear size homeostasis is achieved by a rapid correction mechanism modulating nuclear growth rate in growing fission yeast cells. Nuclear size control Organelle scaling Nucleus Fission yeast Homeostasis Francis Crick Institute 10.13039...

...Tiina Viita; Salla Kyheröinen; Bina Prajapati; Jori Virtanen; Mikko J. Frilander; Markku Varjosalo; Maria K. Vartiainen ABSTRACT In addition to its essential functions within the cytoskeleton, actin also localizes to the cell nucleus, where it is linked to many important nuclear processes from gene...

... used experimental systems as well as in vivo . In this Hypothesis, we highlight known examples of intracellular mechanical challenges in biological systems and focus on the coping mechanisms of two important organelles, the nucleus and mitochondria, for they are the best studied in this aspect...

... or their cleaved cytoplasmic domains. In cancer cells, these nuclear functions contribute to tumor progression and drug resistance. Here, we examined whether the single Drosophila EGFR can also localize to the nucleus. A chimeric EGFR protein fused at its cytoplasmic C-terminus to DNA-binding and transcriptional...

...Cédric Yapo; Anu G. Nair; Jeanette Hellgren Kotaleski; Pierre Vincent; Liliana R. V. Castro ABSTRACT Although it is known that protein kinase A (PKA) in the nucleus regulates gene expression, the specificities of nuclear PKA signaling remain poorly understood. Here, we combined computational...