... © 2023. Published by The Company of Biologists Ltd 2023 http://www.biologists.com/user-licence-1-1/ Chromatin Mechanobiology Nucleus National Institutes of Health R00GM123195 Division of Materials Research https://dx.doi.org/10.13039/100000078 NSF-DMR-1832002...

... been postulated that the unique segmented nucleus of neutrophils functions to assist their rapid migration. Here, we tested this hypothesis by imaging primary human neutrophils traversing narrow channels in custom-designed microfluidic devices. Individuals were given an intravenous low dose...

..., the contribution of CDK to stationary phase remains largely uncharacterized. Here, we examine the role of the sole S. pombe CDK, Cdc2, in the establishment of stationary phase. We show that in stationary phase, nuclear and chromosomal volumes and the nucleus-to-cell volume ratio are reduced, and sister chromatid...

...Sandrine B. Lavenus; Karl W. Vosatka; Alexa P. Caruso; Maria F. Ullo; Ayesha Khan; Jeremy S. Logue ABSTRACT When metastasizing, tumor cells must traverse environments with diverse physicochemical properties. Recently, the cell nucleus has emerged as a major regulator of the transition from...

...Sanju Ashraf; Ye Dee Tay; David A. Kelly; Kenneth E. Sawin ABSTRACT Movement of the cell nucleus typically involves the cytoskeleton and either polymerization-based pushing forces or motor-based pulling forces. In the fission yeast Schizosaccharomyces pombe , nuclear movement and positioning...

... chondrocyte quiescence and immature chondrocyte state through reducing the amount of H3K9me3-marked heterochromatin. Chondrocyte Differentiation Heterochromatin Hydrostatic pressure Mechanotransduction Nucleus Replicative stress The ends of long bones at the regions of joints are covered...

... of adenovirus (AdV) infection. AdV delivers, transcribes and replicates a linear double-stranded DNA genome in the nucleus. We measured the abundance of viral transcripts using single-molecule RNA fluorescence in situ hybridization (FISH) and the incoming 5-ethynyl-2′-deoxycytidine (EdC)-tagged viral genomes...

...Justin T. Jacob; Raji R. Nair; Brian G. Poll; Christopher M. Pineda; Ryan P. Hobbs; Michael J. Matunis; Pierre A. Coulombe ABSTRACT Keratin 17 ( KRT17 ; K17), a non-lamin intermediate filament protein, was recently found to occur in the nucleus. We report here on K17-dependent differences...

... work is properly attributed. Summary: Nuclear size homeostasis is achieved by a rapid correction mechanism modulating nuclear growth rate in growing fission yeast cells. Nuclear size control Organelle scaling Nucleus Fission yeast Homeostasis Membrane-bound organelles increase...

...Tiina Viita; Salla Kyheröinen; Bina Prajapati; Jori Virtanen; Mikko J. Frilander; Markku Varjosalo; Maria K. Vartiainen ABSTRACT In addition to its essential functions within the cytoskeleton, actin also localizes to the cell nucleus, where it is linked to many important nuclear processes from gene...

... used experimental systems as well as in vivo . In this Hypothesis, we highlight known examples of intracellular mechanical challenges in biological systems and focus on the coping mechanisms of two important organelles, the nucleus and mitochondria, for they are the best studied in this aspect...

... or their cleaved cytoplasmic domains. In cancer cells, these nuclear functions contribute to tumor progression and drug resistance. Here, we examined whether the single Drosophila EGFR can also localize to the nucleus. A chimeric EGFR protein fused at its cytoplasmic C-terminus to DNA-binding and transcriptional...

...Cédric Yapo; Anu G. Nair; Jeanette Hellgren Kotaleski; Pierre Vincent; Liliana R. V. Castro ABSTRACT Although it is known that protein kinase A (PKA) in the nucleus regulates gene expression, the specificities of nuclear PKA signaling remain poorly understood. Here, we combined computational...

... for polyphosphoinositide metabolism inside the nucleus which changes with cell differentiation . Biochem. J.   248 , 765 - 770 . 10.1042/bj2480765 Dieck , C. B. , Wood , A. , Brglez , I. , Rojas-Pierce , M. and Boss , W. F. ( 2012 ). Increasing phosphatidylinositol (4,5) bisphosphate...

... events given that the analysis requires components from many cells. Microscopy can visualize single cells, but the cell body and organelles can hamper staining and visualization of the nucleus. Normally, chromatin is visualized by immunostaining a fixed sample or by expressing exogenous fluorescently...

... role of cell adhesion receptors in this process is established, it has only recently been appreciated that mechanical cues reach far beyond the plasma membrane and the cytoskeleton, and are directly transmitted to the nucleus. Furthermore, changes in the mechanical properties of the perinuclear...

.... This demonstrated the importance of image analysis to enhance super-resolution techniques. Structured illumination microscopy Image analysis Chromatin Nucleus Histone modification Nuclear pore complexes The nuclear periphery (NP) represents a complex compartment of substantial importance...

...Leonid A. Serebryannyy; Megan Parilla; Paolo Annibale; Christina M. Cruz; Kyle Laster; Enrico Gratton; Dmitri Kudryashov; Steven T. Kosak; Cara J. Gottardi; Primal de Lanerolle ABSTRACT Actin is abundant in the nucleus and it is clear that nuclear actin has important functions. However, mystery...

... imaging. The camera and microscope settings were kept fixed, to compare different cells. NTIR quantification was performed using a custom-written MATLAB code analyzing 3D stacks of images. The code can be obtained at https://github.com/aneeshsathe/Nucleus-Cell-Ratio . Importing of 3D-image stacks...

... 2016 © 2016. Published by The Company of Biologists Ltd 2016 Summary: NTF2 reduces the diameter of the NPC, nuclear import of large cargos and nuclear size in a Ran-binding-dependent manner, in both Xenopus and mammalian cells. Nucleus NTF2 Nuclear transport Ran Nuclear pore...