..., and chromatin in particular, respond to changes in the mechanical microenvironment of the cell, resulting in architectural and transcriptional alterations, and thereby facilitating cellular adaption. Mechanotransduction Nucleus Nuclear lamina Nucleoskeleton Nuclear mechanical response Max...

... The authors declare no competing interests. 18 12 2013 8 07 2014 © 2014. Published by The Company of Biologists Ltd 2014 Barrier to autointegration factor Emerin Emery-Dreifuss muscular dystrophy Lamin LEM domain Nestor-Guillermo progeria Nuclear envelope Nucleoskeleton...

... between the concentration of ECM components and bulk tissue elasticity was discovered for mouse tissues ( Swift et al., 2013b ). More surprisingly, we also discovered a systematic scaling between tissue elasticity and the concentration of lamins in the nucleoskeleton that was partially re-capitulated...

...., Raymond, Y. and Cook, P. R. ( 1995 ). Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells. J. Cell Sci. 108 , 635 -644. Jackson, D. A. and Cook, P. R. ( 1985 ). Transcription occurs at a nucleoskeleton. EMBO J. 4 , 919 -925. Jackson, D...

... of chromosome territories, which relocate towards the nuclear periphery. These results show that a lamin B1-containing nucleoskeleton is required to maintain RNA synthesis and that ongoing synthesis is a fundamental determinant of global nuclear architecture in mammalian cells. Cells with lamin B1 expression...

... myogenesis, Rb was rapidly and progressively dephosphorylated. Underphosphorylated Rb formed complexes with LAP2α in proliferating myoblasts and postmitotic myoblasts. In myoblasts transfected with the mutant lamins, this complex was disrupted. These data suggest that remodelling of the nucleoskeleton...

... morphology in male meiosis. We propose that EAST constitutes a component of a nucleoskeleton that helps to constrain the mobility of chromosomes in interphase, mitosis and meiosis. * Author for correspondence (e-mail: [email protected] ) 21 1 2003 © The Company of Biologists Limited...

...J. Miguel Ortega; Melvin L. DePamphilis ABSTRACT To determine whether or not initiation sites for DNA replication in mammalian cells are defined by association with nuclear structure, attachments between the nucleoskeleton and the hamster DHFR gene initiation zone were examined. Nucleoskeletons...

... The Company of Biologists Limited 1995 1995 nucleoskeleton replication transcription repair chromatin loop Compared to the compartmentation found within the cytoplasm, the nucleus has traditionally been viewed as relatively unstructured. However, recent experiments suggest that it also has...

... sections from which ∼90% chromatin had been removed showed that most DNA synthesis occurs in specific dense structures (replication factories) attached to a diffuse nucleoskeleton. These factories appear at the end of G 1 -phase and quickly become active; as S-phase progresses, they increase in size...

... nucleoskeleton. After treatment with an endonuclease, most repaired DNA electroeluted from beads with chromatin fragments; this was unlike nascent DNA made during S phase and suggests that repaired DNA is not as closely associated with the skeleton. However, the procedure destroyed repair activity, so repaired...

... and nucleosome assembly, and may reflect the attachment of the yeast DNA to the rodent cell nucleoskeleton. The yeast integrant replicates late in S phase at a time when G bands of the mouse chromosomes are being replicated, and participates in sister chromatid exchanges at a high frequency. We discuss...

.... These results suggest that the dense fibrillar component is the site of rRNA transcription. After dispersing the granular component and the dense fibrillar component by a hypotonic treatment, removal of most chromatin and preparation of resinless sections, fibrillar centres remained fixed to a nucleoskeleton...

... was very stable. Foci remained when ∼90% chromatin was removed, suggesting they were attached to an underlying structure. * Author for correspondence 22 01 1993 24 02 1993 © 1993 by Company of Biologists 1993 replication foci DNA polymerase nucleoskeleton dTTP analogues...

... domain. * Author for correspondence © 1991 by Company of Biologists 1991 method chromatin nucleoskeleton DNA fractionation In eukaryotic cells linear DNA is organized into arrays of supercoiled loops tightly attached to a proteinaceous skeleton structure (reviewed by Agutter...

..., but their chromatin is accessible to restriction enzymes and so probably to most enzymes used in molecular biology. We hope that these encapsulated derivatives of cells, well-preserved and containing intact DNA, cytoskeleton and nucleoskeleton, accessible, yet expressing in vitro the major nuclear functions...