...Gabriel P. Faber; Shani Nadav-Eliyahu; Yaron Shav-Tal ABSTRACT Nuclear speckles are dynamic membraneless bodies located in the cell nucleus. They harbor RNAs and proteins, many of which are splicing factors, that together display complex biophysical properties dictating nuclear speckle formation...

... containing the speckle marker protein SON. Similar long-range speckle movements and fusion events were observed after heat shock or heavy metal stress, and during late G2 and early prophase. Our observations suggest a mechanism for long-range, directional nuclear speckle movements, contributing to overall...

.... * Author for correspondence ([email protected]) 9 7 2012 © 2012. Published by The Company of Biologists Ltd 2012 TFII-I Transposon Transgenic Muscle Nuclear speckles GTF2IRD2 belongs to a small family of related genes – GTF2I , GTF2IRD1 and GTF2IRD2...

...) proteins to be functional. These insulator proteins are colocalized in nuclear speckles named insulator bodies. Here, we demonstrate that post-translational modification of insulator proteins by small ubiquitin-like modifier (SUMO) and intact CP190 protein is crucial for insulator body formation...

... that are essential for maintaining the subnuclear organization of pre-mRNA processing factors in nuclear speckles. Motif analysis of Son highlights putative RNA interaction domains that suggest a direct role for Son in pre-mRNA splicing. Here, we used in situ approaches to show that Son localizes to a reporter...

... in nuclear speckles, a sub-nuclear compartment enriched in small nuclear ribonucleoproteins and splicing factors. In vitro methylation assays indicated that hCAF1 is not a substrate for PRMT1-mediated methylation, but it regulates PRMT1 activity in a substrate-dependent manner. Moreover, small interfering...

..., hOGG1 is recruited from a soluble nucleoplasmic localisation to the nuclear matrix. More specifically, after irradiation, hOGG1 forms foci colocalising with the nuclear speckles, organelles that are interspersed between chromatin domains and that have been associated with transcription and RNA-splicing...

...-activated receptor γ. FAD24 localizes in the nucleus, especially within nuclear speckles. As the nuclear speckle functions as a nascent transcription and pre-mRNA splicing machinery, there is a possibility that FAD24 functions as one of the components for transcription and/or pre-mRNA splicing...

... the localisation of RS proteins. The SRPK1 protein kinase is specific for RS proteins (Gui et al., 1994b) and can cause the disassembly of nuclear speckles (Gui et al., 1994a). Furthermore, high levels of SRPK1 inhibit an in vitro splicing reaction (Gui et al., 1994a). The Clk/Sty protein kinase has a less...