.... © 2014. Published by The Company of Biologists Ltd 2014 O -GlcNAc O -GlcNAcylation Metabolism Post-translational modification Signaling Lipid droplets Mitochondria Epigenetics Nuclear pores Hundreds of proteins are modified at the hydroxyl group of their serine and threonine...

...Kazuhiro Maeshima; Kazuhide Yahata; Yoko Sasaki; Reiko Nakatomi; Taro Tachibana; Tsutomu Hashikawa; Fumio Imamoto; Naoko Imamoto Nuclear pores are sophisticated gateways on the nuclear envelope that control macromolecular transport between the cytoplasm and nucleoplasm. So far the structural...

... of the envelope and the relationship between nuclear pores, lamina fibres and pore-connecting fibrils. The lamin proteins that constitute the lamina resemble intermediate filament proteins, and assemble and disassemble during mitosis in response to phosphorylation. Lamins have been expressed in E. coli...

... by amyl acetate. These methods emphasized different aspects of the structure and enabled an integrated view of the arrangement of nuclear pore complexes, nuclear lamina and pore-connecting fibrils to be assembled. In specimens prepared by either airdrying or freeze-drying, the lamina meshwork beneath...

..., clusters of nuclear pores, closely paralleling the clustering of membrane-attached chromatin fibres, have been identified on the envelope, the number of these being similar to the number of homologus pairs of metaphase chromosomes. Furthermore, rapid changes induced in chromatin distribution appear...