.... LINC complexes are formed by a transluminal interaction between the outer and inner nuclear membrane KASH and SUN proteins, respectively. Despite recent structural insights, our mechanistic understanding of LINC complex assembly remains limited by the lack of an experimental system for its in vitro...

...Veronica J. Larsson; Mohammed Hakim Jafferali; Balaje Vijayaraghavan; Ricardo A. Figueroa; Einar Hallberg ABSTRACT We have investigated a possible role for the inner nuclear membrane protein Samp1 (also known as TMEM201) in the mitotic machinery. Live-cell imaging showed that Samp1a–YFP (Samp1a...

...Mirta Boban; Marina Pantazopoulou; Anna Schick; Per O. Ljungdahl; Roland Foisner ABSTRACT The nuclear envelope consists of inner and outer nuclear membranes. Whereas the outer membrane is an extension of the endoplasmic reticulum, the inner nuclear membrane (INM) represents a unique membranous...

...Kiran Busayavalasa; Xin Chen; Ann-Kristin Östlund Farrants; Nicole Wagner; Nafiseh Sabri Summary The nuclear envelope (NE), an important barrier between the nucleus and the cytoplasm, is composed of three structures: the outer nuclear membrane, which is continuous with the ER, the inner nuclear...

... in a perinuclear distribution, consistent with its known localization in the endoplasmic reticulum (ER) and the contiguous outer nuclear membrane. Here, we report that, in addition to its localization at the ER, PTP1B also is found at the inner nuclear membrane, where it is heavily sumoylated. We also find...

...Santhosh Gudise; Ricardo A. Figueroa; Robert Lindberg; Veronica Larsson; Einar Hallberg The transmembrane inner nuclear membrane (INM) protein Samp1 is required for anchoring centrosomes near the nuclei. Using high-resolution fluorescence microscopy we show that Samp1 is distributed in a distinct...

... family, which includes the mouse Lpin genes, one of whose mutations is responsible for lipodystrophy. * Author for correspondence (e-mail: [email protected] ) 29 8 2002 © The Company of Biologists Limited 2002 2002 Ran Lipin Nuclear membrane A variety of genes...

...John M. K. Mislow; Marian S. Kim; Dawn Belt Davis; Elizabeth M. McNally Mutations in the genes encoding the inner nuclear membrane proteins lamin A/C and emerin produce cardiomyopathy and muscular dystrophy in humans and mice. The mechanism by which these broadly expressed gene products result...

...-mail: [email protected] ) 9 7 2001 © The Company of Biologists Limited 2001 2001 Apoptosis Nuclear lamina Nuclear envelope Nuclear membrane Nuclear pore complex The nuclear envelope (NE) separates the nucleus from the cytoplasm ( Gerace and Burke, 1988 ; Stoffler et al., 1999...

... (LB) for the lamina, transmembrane proteins LBR and LAP2 for the inner nuclear membrane, and nucleoporins p62, Nup153 and gp210 for the nuclear pore complexes (NPCs), we observed a selective and conserved cleavage of LB, LAP2 and Nup153. In lymphoid cells, the rate of cleavage of these markers...

.... Although the presence of pores in the nuclear membrane has been taken as evidence that free diffusion of ions and small molecules can occur in and out the nucleus, we conclude that the nuclear membrane of these cells presents a permeability barrier to H + . The pH gradient was not observed in cells...

... spindle pole body. Therefore, spindle pole body duplication seemed to require functional β-tubulin. The nuclear membrane was highly deformed with a leaf-like profile in cross-section, possibly due to an interaction with the rod-like, condensed chromosomes. Upon transfer to the permissive temperature...