... wherein the protein CHIP (C-terminus of Hsc70-interacting protein) mediates PTEN monoubiquitylation, leading to its nuclear import. Western blot analysis revealed a rise in both nuclear and total cellular PTEN levels under monoubiquitylation-promoting conditions, an effect that was abrogated by silencing...

... characterize the nuclear import of the human CHD4 protein. We show that CHD4 enters the nucleus by means of several importin-α proteins (1, 5, 6 and 7), but independently of importin β1. Importin α1 directly interacts with a monopartite ‘KRKR’-motif in the N-terminus of CHD4 (amino acids 304–307). However...

... physically interacts with proteasome proteins, and Ipo9 mutant males exhibit disruption of the nuclear localization of several proteasome components. Thus, Ipo9 coordinates the nuclear import of functionally related factors necessary for the completion of gametogenesis. This article has an associated First...

... within 20 min of reformation of the nucleus, after which import comes to an abrupt halt. This suggests that maintaining the nuclear–cytosol distribution after mitosis requires chromatin condensation to exclude cytosolic material from the nuclear space, and specialized machineries for nuclear import...

... for the broad range of cellular functions of the protein is incompletely understood. We show that HuR controls the expression of multiple components of the nuclear import machinery. Consequently, HuR is crucial for the nuclear import of cellular retinoic acid-binding protein 2 (CRABP2), which delivers RA...

... to this canonical nuclear import pathway. Here, we report a new unconventional nuclear import mechanism exploited by the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV). We found that AcMNPV nucleocapsids entered the nucleus of digitonin-permeabilized cells in the absence of exogenous...

... formation of FUS in ALS and FTLD. Here, we show that the C-terminal tyrosine residue at position 526 of FUS is crucial for normal nuclear import. This tyrosine is subjected to phosphorylation, which reduces interaction with transportin 1 and might consequentially affect the transport of FUS into the nucleus...

... leukemias and solid tumor formation. As a regulated transcription factor, precise cellular localization of STAT5 is essential. Conventional nuclear localization signals consist of short stretches of basic amino acids. In this study, we provide evidence that STAT5 nuclear import is dependent...

... factor for transportin-mediated nuclear import. Depletion of Nup358 resulted in a strong inhibition of nuclear import of the human immunodeficiency virus type 1 (HIV-1) Rev protein. HIV-1 Rev is an RNA-binding protein that is required for CRM1 (also known as exportin 1)-dependent nuclear export...

...Mark L. Cutress; Hayley C. Whitaker; Ian G. Mills; Murray Stewart; David E. Neal Ligand-dependent nuclear import is crucial for the function of the androgen receptor (AR) in both health and disease. The unliganded AR is retained in the cytoplasm but, on binding 5α-dihydrotestosterone...

... of the cell. Cell 105 , 697 -700. Day, J., Kuznetsov, Y. G., Larson, S. B., Greenwood, A. and McPherson, A. ( 2001 ). Biophysical studies on the RNA cores of satellite tobacco mosaic virus. Biophys. J. 80 , 2364 -2371. Greber, U. F. and Fassati, A. ( 2003 ). Nuclear import of viral DNA...

... nuclear import is transcription-dependent. We identified RRM2 and the first half of the auxiliary region as important determinants for TIAR and TIA-1 nuclear accumulation. In contrast, the nuclear export of TIAR and TIA-1 is mediated by RRM3. Both RRMs contribute to TIAR and TIA-1 nuclear accumulation...

... leukemia virus type I Tax and CREB. J. Virol. 69 , 3420 -3432. * Author for correspondence (e-mail: [email protected] ) 13 1 2005 © The Company of Biologists Limited 2005 2005 HTLV-I HBZ bZIP protein Nuclear import Heterochromatin Basic...

...-receptor-clustering gene rapsyn . Similar to other BTB/POZ proteins (e.g. Bcl-6, PLZF, HIC-1), endogenous Kaiso localizes predominantly to the nuclei of mammalian cells. To date, however, the mechanism of nuclear import for most POZ transcription factors, including Kaiso, remain unknown. Here, we report...

... sequence-tagged CapG-EGFP fails to induce invasion, whereas point mutations in the nuclear export sequence permitting nuclear re-entry restore cellular invasion. Nuclear import of CapG is energy-dependent and requires the cytosolic receptor importin β but not importin α. Nuclear CapG does not possess...

... of Gent, K.L. Ledeganckstraat 35, 9000 Gent, Belgium 22 4 2004 © The Company of Biologists Limited 2004 2004 TGF-β Smad Nucleocytoplasmic shuttling GFP Nuclear import Nuclear export Photobleaching Signals from receptors for Transforming growth factor β (TGF-β...

... of Biologists Limited 2004 2004 p120 ctn Kaiso NLS Nuclear import Transcriptional repression The Armadillo catenins, β-, γ- and p120 ctn , are multifunctional proteins whose malfunction contributes to tumor progression by various means ( Behrens, 1999 ; Van Aken et al., 2001...

... within the putative nuclear localization signal, T445, is required for efficient nuclear import of a GST-carboxy-tail fusion, two others, S421 and S423, appear to effect release from the import receptors. Although overexpression of recombinant HDACm in oocytes leads to premature condensation...

... assays, and our results suggest that the interaction between different Hrp65 isoforms is crucial for their intracellular localization. Fig. 6. Nuclear import of Hrp65 isoforms analyzed by transient transfection assays. GFP fused to either Hrp65-1 or to Hrp65 amino acids 1-499, and Hrp65-2 fused...

... in the context of the Inh2-GFP 3 protein but did not see a significant reduction in nuclear import( Fig. 7 ). Fig. 7. Inhibitor-2 is actively imported into the nucleus. A and B show low-density HeLa cells expressing either an Inh2GFP 3 fusion (A) or an Inh2GFP 3 fusion in which lysines 142 and 144...