... of substrate stiffnesses and extracellular matrix components to understand their effects on myoblast contractility, which was found to be linked to proliferation. Myoblast Substrate stiffness Nuclear envelope Cell contractility Extracellular matrix Mechanobiology Natural Sciences...

...Sarah R. Barger; Lauren Penfield; Shirin Bahmanyar ABSTRACT Barrier-to-autointegration factor (BAF) protein is a DNA-binding protein that crosslinks chromatin to allow mitotic nuclear envelope (NE) assembly. The LAP2-emerin-MAN1 (LEM)-domain protein LEMD2 and ESCRT-II/III hybrid protein CHMP7 close...

...Yasuhiro Hirano; Yusuke Ohno; Yoshino Kubota; Tatsuo Fukagawa; Akio Kihara; Tokuko Haraguchi; Yasushi Hiraoka ABSTRACT Maintaining the integrity of the nuclear envelope (NE) is essential for preventing genomic DNA damage. Recent studies have shown that enzymes that catalyze lipid synthesis...

...Hanna Thoma; Luisa Grünewald; Silke Braune; Elisabeth Pasch; Manfred Alsheimer ABSTRACT SUN domain proteins are conserved proteins of the nuclear envelope and key components of the LINC complexes (for ‘linkers of the nucleoskeleton and the cytoskeleton’). Previous studies have demonstrated...

...Sherman Foo; Amaury Cazenave-Gassiot; Markus R. Wenk; Snezhana Oliferenko ABSTRACT Nuclear envelope (NE) expansion must be controlled to maintain nuclear shape and function. The nuclear membrane expands massively during closed mitosis, enabling chromosome segregation within an intact NE...

... of an A375-M2 cell confined down to ∼3 µm, transiently transfected with emerin-EGFP and stained with membrane dye. Panels on the right (Zoom) show emerin predominantly located at the nuclear envelope and ER. (A′) Line scan (green line) highlighting the enrichment of emerin at the nuclear envelope (NE...

...Anthony Fernandez; Markville Bautista; Liying Wu; Fabien Pinaud ABSTRACT Emerin is an integral nuclear envelope protein that participates in the maintenance of nuclear shape. When mutated or absent, emerin causes X-linked Emery-Dreifuss muscular dystrophy (EDMD). To understand how emerin takes part...

...Zeinab Jahed; Nya Domkam; Jessica Ornowski; Ghafar Yerima; Mohammad R. K. Mofrad ABSTRACT Large protein complexes assemble at the nuclear envelope to transmit mechanical signals between the cytoskeleton and nucleoskeleton. These protein complexes are known as the linkers of the nucleoskeleton...

... in interphase and becomes dumbbell shaped during mitotic division to facilitate the transfer of one nucleus to the daughter cell. Because yeast cells undergo closed mitosis, the nuclear envelope remains intact throughout the cell cycle. The pathways that regulate nuclear shape are not well characterized...

... DNA, histones and various other nuclear proteins, including those enriched at the nuclear envelope, BAF appears to be essential for replicating cells to protect the genome and enable cell division. Cellular processes, such as innate immunity, post-mitotic nuclear reformation, repair of interphase...

... in glycosylphosphatidylinositol (GPI), localizes to the nuclear envelope (NE). Although this NE localization is essential for Drosophila development, the underlying molecular mechanism remains unknown. To elucidate this mechanism, we identified PIG-B-interacting proteins by performing immunoprecipitation followed by proteomic...

..., the nuclear envelope possesses unique mechanical properties, which enables it to resist cytoskeletal forces. Here, we review studies that are beginning to provide quantitative insights into nuclear membrane mechanics. We discuss how the mechanical properties of the fused nuclear membranes mediate...

... causes lethal defects associated with loss of the nuclear barrier function; the very-long-chain fatty acid elongase Elo2 rescues such lethal defects. Elo2 Fatty acid elongation Fission yeast Lem2 Nuclear envelope Japan Society for the Promotion of Science (KAKENHI) JP25840008...

...Menno Spits; Lennert J. Janssen; Lenard M. Voortman; Raymond Kooij; Anna C. M. Neefjes; Huib Ovaa; Jacques Neefjes ABSTRACT Upon nuclear envelope (NE) fragmentation in the prometaphase, the nuclear and cytosolic proteomes mix and must be redefined to reinstate homeostasis. Here, by using...

...Hardeep K. Gumber; Joseph F. McKenna; Amado L. Estrada; Andrea F. Tolmie; Katja Graumann; Hank W. Bass ABSTRACT The linker of nucleoskeleton to cytoskeleton (LINC) complex is an essential multi-protein structure spanning the nuclear envelope. It connects the cytoplasm to the nucleoplasm, functions...

..., is synthesized by the PIG enzymes. Most PIGs are localized to the endoplasmic reticulum (ER), but Drosophila PIG-B (DmPIG-B) is localized to the nuclear envelope (NE). To determine whether the NE localization of DmPIG-B is functionally important, we defined the determinants of localization and generated an ER...

...: Nuclei are highly branched throughout the heterogeneous population of healthy epidermal cells in the Xenopus tail fin periphery, and disruption of nuclear branching results in improper fin morphology. Nuclear morphology Nuclear envelope Lamin Actin Xenopus tropicalis Epidermis...

.... , Takagi , T. , Osumi , M. , Kimura , A. and Niki , H. ( 2011 ). Breakage of the nuclear envelope by an extending mitotic nucleus occurs during anaphase in Schizosaccharomyces japonicus . Genes Cells   16 , 911 - 926 . 10.1111/j.1365-2443.2011.01540.x Arai , K. , Sato , M...

.... Summary: There are compositional and structural differences in the nuclear pore complexes present in the functionally differentiated macronucleus and micronucleus within the single cytoplasm of ciliated protozoa. FG-Nup Nuclear dimorphism Nuclear envelope Nucleoporin Y-complex Japan...

...Marta G. Lete; Richard D. Byrne; Alicia Alonso; Dominic Poccia; Banafshé Larijani ABSTRACT Regulation of nuclear envelope dynamics is an important example of the universal phenomena of membrane fusion. The signalling molecules involved in nuclear membrane fusion might also be conserved during...