...Yasuhiro Hirano; Yusuke Ohno; Yoshino Kubota; Tatsuo Fukagawa; Akio Kihara; Tokuko Haraguchi; Yasushi Hiraoka ABSTRACT Maintaining the integrity of the nuclear envelope (NE) is essential for preventing genomic DNA damage. Recent studies have shown that enzymes that catalyze lipid synthesis...

...Hanna Thoma; Luisa Grünewald; Silke Braune; Elisabeth Pasch; Manfred Alsheimer ABSTRACT SUN domain proteins are conserved proteins of the nuclear envelope and key components of the LINC complexes (for ‘linkers of the nucleoskeleton and the cytoskeleton’). Previous studies have demonstrated...

...Sherman Foo; Amaury Cazenave-Gassiot; Markus R. Wenk; Snezhana Oliferenko ABSTRACT Nuclear envelope (NE) expansion must be controlled to maintain nuclear shape and function. The nuclear membrane expands massively during closed mitosis, enabling chromosome segregation within an intact NE...

.... and Paluch , E. K. ( 2015 ). Force transmission during adhesion-independent migration . Nat. Cell Biol. 17 , 524 - 529 . 10.1038/ncb3134 Berk , J. M. , Tifft , K. E. and Wilson , K. L. ( 2013 ). The nuclear envelope LEM-domain protein emerin . Nucleus 4 , 298 - 314...

...Anthony Fernandez; Markville Bautista; Liying Wu; Fabien Pinaud ABSTRACT Emerin is an integral nuclear envelope protein that participates in the maintenance of nuclear shape. When mutated or absent, emerin causes X-linked Emery-Dreifuss muscular dystrophy (EDMD). To understand how emerin takes part...

...Zeinab Jahed; Nya Domkam; Jessica Ornowski; Ghafar Yerima; Mohammad R. K. Mofrad ABSTRACT Large protein complexes assemble at the nuclear envelope to transmit mechanical signals between the cytoskeleton and nucleoskeleton. These protein complexes are known as the linkers of the nucleoskeleton...

... in interphase and becomes dumbbell shaped during mitotic division to facilitate the transfer of one nucleus to the daughter cell. Because yeast cells undergo closed mitosis, the nuclear envelope remains intact throughout the cell cycle. The pathways that regulate nuclear shape are not well characterized...

... DNA, histones and various other nuclear proteins, including those enriched at the nuclear envelope, BAF appears to be essential for replicating cells to protect the genome and enable cell division. Cellular processes, such as innate immunity, post-mitotic nuclear reformation, repair of interphase...

... in glycosylphosphatidylinositol (GPI), localizes to the nuclear envelope (NE). Although this NE localization is essential for Drosophila development, the underlying molecular mechanism remains unknown. To elucidate this mechanism, we identified PIG-B-interacting proteins by performing immunoprecipitation followed by proteomic...

..., the nuclear envelope possesses unique mechanical properties, which enables it to resist cytoskeletal forces. Here, we review studies that are beginning to provide quantitative insights into nuclear membrane mechanics. We discuss how the mechanical properties of the fused nuclear membranes mediate...

...Menno Spits; Lennert J. Janssen; Lenard M. Voortman; Raymond Kooij; Anna C. M. Neefjes; Huib Ovaa; Jacques Neefjes ABSTRACT Upon nuclear envelope (NE) fragmentation in the prometaphase, the nuclear and cytosolic proteomes mix and must be redefined to reinstate homeostasis. Here, by using...

... causes lethal defects associated with loss of the nuclear barrier function; the very-long-chain fatty acid elongase Elo2 rescues such lethal defects. Elo2 Fatty acid elongation Fission yeast Lem2 Nuclear envelope Fatty acids are components of lipids, which constitute the intracellular...

...Hardeep K. Gumber; Joseph F. McKenna; Amado L. Estrada; Andrea F. Tolmie; Katja Graumann; Hank W. Bass ABSTRACT The linker of nucleoskeleton to cytoskeleton (LINC) complex is an essential multi-protein structure spanning the nuclear envelope. It connects the cytoplasm to the nucleoplasm, functions...

..., is synthesized by the PIG enzymes. Most PIGs are localized to the endoplasmic reticulum (ER), but Drosophila PIG-B (DmPIG-B) is localized to the nuclear envelope (NE). To determine whether the NE localization of DmPIG-B is functionally important, we defined the determinants of localization and generated an ER...

... at various stages and stained with Lamin B1 antibody to show the nuclear periphery and, chromatin labeled with DAPI. We found that by late neurula stages (NF stage 18), the nuclear envelope is ruffled and irregular, though the chromatin distribution is still largely ellipsoid ( Fig. 1 F). We note...

... approaches used in comparative biology studies. Chromosome segregation Comparative biology Evolution Mitosis Nuclear envelope Spindle The generality of cell biological mechanisms has become a go-to argument for researchers explaining the relevance of their work to funding bodies...

.... Summary: There are compositional and structural differences in the nuclear pore complexes present in the functionally differentiated macronucleus and micronucleus within the single cytoplasm of ciliated protozoa. FG-Nup Nuclear dimorphism Nuclear envelope Nucleoporin Y-complex Ciliated...

...Marta G. Lete; Richard D. Byrne; Alicia Alonso; Dominic Poccia; Banafshé Larijani ABSTRACT Regulation of nuclear envelope dynamics is an important example of the universal phenomena of membrane fusion. The signalling molecules involved in nuclear membrane fusion might also be conserved during...

.... SIRT2 has also been implicated in the pathology of cancer, neurodegenerative diseases and progeria. Here, we show that SIRT2 depletion or overexpression causes nuclear envelope reassembly defects. We link this phenotype to the recently identified regulator of nuclear envelope reassembly ANKLE2. ANKLE2...

...Marion Weberruss; Wolfram Antonin ABSTRACT The nucleus is enclosed by the nuclear envelope, a double membrane which creates a selective barrier between the cytoplasm and the nuclear interior. Its barrier and transport characteristics are determined by nuclear pore complexes (NPCs) that are embedded...