... domain of Cep215 binds Cep192 and phosphorylated Aurora A to play a role in maintaining the structural integrity of spindle poles during mitosis. Centrosomes Spindle poles Cep215 Cep192 Phosphorylated Aurora A NuMA National Science Foundation 10.13039/100000001 MCB1140033...

...Riya Keshri; Ashwathi Rajeevan; Sachin Kotak ABSTRACT Proper orientation of the mitotic spindle is critical for accurate development and morphogenesis. In human cells, spindle orientation is regulated by the evolutionarily conserved protein NuMA, which interacts with dynein and enriches...

...), NuMA (also known as NUMA1) and dynein–dynactin, plays a key role in establishing proper spindle orientation. It has also been shown that the actin-binding protein MISP and the ERM family, which are activated by lymphocyte-oriented kinase (LOK, also known as STK10) and Ste20-like kinase (SLK) (hereafter...

... localization at the cell cortex. Moreover, we show that Aurora A and the PP6 phosphatase subunit PPP6C are also necessary for spindle positioning in human cells. There, Aurora A is needed for the cortical localization of NuMA and dynein during mitosis. Overall, our work demonstrates that Aurora A kinases...

... splicing factor speckles, and distinct nuclear mitotic apparatus protein (NuMA) foci. Upon alteration of nuclear organization using an antibody against NuMA, differentiated non-neoplastic cells undergo apoptosis, whereas partially differentiated malignant cells enter the cell cycle. Non-neoplastic cells...

...Olga Kisurina-Evgenieva; Gary Mack; Quansheng Du; Ian Macara; Alexey Khodjakov; Duane A. Compton The large coiled-coil protein NuMA plays an essential role in organizing microtubule minus ends at spindle poles in vertebrate cells. Here, we use both in vivo and in vitro methods to examine NuMA...

...Pekka Taimen; Markku Kallajoki NuMA is a nuclear matrix protein that has an essential function in the organization of the mitotic spindle. Here we have studied the fate of NuMA in Fas-treated apoptotic Jurkat T and HeLa cells. We show that in both cell lines NuMA is an early target protein...

... nucleotide exchange factor RCC1, stabilises large microtubule asters nucleated at the sperm centrosome, prevents the redistribution of NuMA from the aster to the nucleus and blocks chromatin decondensation. In contrast, Ran GDP does not stabilise microtubules or inhibit nuclear assembly. RanT24N and RanBP1...

... redistribution of the protein during mitosis, B23 also becomes associated with mitotic spindle poles starting from early prometaphase onwards. Colocalization of B23 with the protein NuMA (Nuclear Mitotic Apparatus protein) was studied in mitotic cells and taxol-arrested cells. During the onset of mitosis, we...

... of apoptosis to double that of spontaneous apoptosis in controls within 6 hours. Immunolabelling for Nuclear Mitotic Apparatus Protein (NuMA) suggested that splenocytes were responding heterogeneously to the heat treatment. Whereas all nuclei in controls and about half of nuclei in heat-treated samples showed...

...Andreas Merdes; Don W. Cleveland ABSTRACT NuMA is an essential protein for the formation of spindle poles in mitosis. During interphase, NuMA is transported into the nucleus where it resides until prometaphase of the next mitotic cycle. We tested for a potential function of NuMA in interphase...

...Alejandro Saredi; Louisa Howard; Duane A. Compton ABSTRACT NuMA is a 236 kDa nuclear protein that is required for the organization of the mitotic spindle. To determine how NuMA redistributes in the cell during mitosis, we have examined the behavior of NuMA in a mammalian mitotic extract under...

...Alejandro Saredi; Louisa Howard; Duane A. Compton ABSTRACT NuMA is a 236 kDa protein that participates in the organization of the mitotic spindle despite its strict localization in the nucleus during interphase. To test how cells progress through mitosis when NuMA is localized in the cytoplasm...

...Hsin-Ling Hsu; Ning-Hsing Yeh ABSTRACT We have demonstrated that dynamic redistribution of nuclear-mitotic apparatus (NuMA) protein in the cell cycle is correlated temporally and spatially with its biochemical modifications. In interphase, NuMA behaves solely as a 220 kDa nuclear matrix-associated...

... fractions were isolated and western blot analysis was performed with an anti-NuMA antibody, which was generated against the 96 kDa C-terminal fragment of the recombinant protein (Fig. 5). The antibody specifically recognized a 240 kDa single band of the native NuMa protein in matrix fractions prepared from...

...C. A. Sparks; E. G. Fey; C. A. Vidair; S. J. Doxsey ABSTRACT NuMA, the nuclear mitotic apparatus protein, is a component of the nuclear matrix at interphase that redis-tributes to the spindle poles at mitosis. While the function of NuMA is not known, it has been implicated in spindle organization...

...Duane A. Compton; Chenghua Luo ABSTRACT NuMA is a 236 kDa intranuclear protein that is distributed into each daughter cell during mitosis through association with the pericentrosomal region of the mitotic spindle. NuMA’s interaction with the microtubules of the mitotic spindle is mediated through...

...Tang K. Tang; Chieh-ju C. Tang; Yu-Jane Chao; Cheng-Wen Wu ABSTRACT We have recently shown that the nuclear mitotic apparatus protein (NuMA) is composed of at least three isoforms that differ mainly at the carboxy terminus, and are generated by alternative splicing of a common mRNA precursor from...

... and carboxy termini. The sequence contained five repeats of the hypothetical leucine zipper motif: one is in the N-terminal globular domain, and four are in the central -helical stalk. Comparison with other sequences in the database shows that the SP-H antigen is identical to the NuMA protein reported by Yang...

... the entire coding region, revealed that the W1 polypeptide was identical to the Nuclear Mitotic Apparatus (NuMA) protein, with a long a-helical central core flanked by two nonhelical domains. Interestingly, most cDNA sequences were identical to each other, except for six sequence blocks which were either...