... used ECPT to characterise different phenotypes in arterial, venous and microvascular EC populations. In line with other studies, we measured heterogeneity in terms of cell cycle, proliferation, and junction organisation. ECPT uncovered a previously under-appreciated single-cell heterogeneity in NOTCH...

...Mirren Charnley; Mandy Ludford-Menting; Kim Pham; Sarah M. Russell; Ana-Maria Lennon-Duménil ABSTRACT A fundamental question in biology is how single cells can reliably produce progeny of different cell types. Notch signalling frequently facilitates fate determination. Asymmetric cell division (ACD...

...Pang-Kuo Lo; Yi-Chun Huang; David Corcoran; Renjie Jiao; Wu-Min Deng ABSTRACT Chromatin assembly factor 1 (CAF1), a histone chaperone that mediates the deposition of histone H3/H4 onto newly synthesized DNA, is involved in Notch signaling activation during Drosophila wing imaginal disc development...

.... Finally, we found that the NOTCH3 mRNA half-life increased during differentiation or upon acute p38-MAPK activation. These results reveal a new connection between p38-MAPK, MYC and NOTCH signaling, demonstrate two mechanisms of NOTCH3 regulation and provide evidence for NOTCH3 involvement in prostate...

... experiments have established that Msi-1 is a Wnt target, thus positioning Msi-1 and Apc as mutual antagonists in a mutually repressive feedback loop. Here, we report that intestines from mice lacking Msi-1 display aberrant Apc and Msi-1 mutually repressive feedback, reduced Wnt and Notch signaling, decreased...

..., and we show that Ras activity is responsible for specific Rabex-5 hematopoietic phenotypes. Surprisingly, Ras-independent Notch protein accumulation and transcriptional activity in the lymph gland underlie multiple distinct hematopoietic phenotypes of Rabex-5 loss. Thus, Rabex-5 plays an important role...

... cell p63 Notch Skin self-renews throughout life by constantly replenishing keratinocytes in the outermost layer of the epidermis in a process that involves balancing the production of stem cells and transit amplifying cells, which are the committed keratinocyte precursors ( Jones and Watt...

... by Igfbp-3 proteolysis. Knockdown of papp-a2 additionally resulted in angiogenesis defects, strikingly similar to previous observations in embryos with mutations in components of the Notch system. Accordingly, we find that Notch signaling is modulated by Papp-a2 in vivo , and, furthermore, that human PAPP...

...Guillaume Pézeron; Kat Millen; Hadi Boukhatmi; Sarah Bray ABSTRACT There is growing evidence that activation of the Notch pathway can result in consequences on cell morphogenesis and behaviour, both during embryonic development and cancer progression. In general, Notch is proposed to coordinate...

... occur outside of the niche and it is unclear how these divisions are controlled. Our data distinguish two new domains outside of the stem cell niche where there are differing controls on proliferation. One domain abuts the niche and is characterised by ESCRT, Notch and JAK/STAT-mediated control...

...Carmen C. Leitch; Sukanya Lodh; Victoria Prieto-Echagüe; Jose L. Badano; Norann A. Zaghloul ABSTRACT Proteins associated with primary cilia and basal bodies mediate numerous signaling pathways, but little is known about their role in Notch signaling. Here, we report that loss of the Bardet-Biedl...

... of transmembrane receptors. The arrestin family comprises visual arrestins, β-arrestins and α-arrestins. In mammals, the functions of the α-arrestins are beginning to be elucidated, and they are described as versatile adaptors that link GPCRs or the Notch receptor to E3 ubiquitin ligases and endocytic factors...

...Loredana Puca; Patricia Chastagner; Vannary Meas-Yedid; Alain Israël; Christel Brou Summary Notch signaling is a conserved signaling pathway implicated in embryogenesis and adult tissue maintenance. Notch signaling strength is strictly regulated, notably by maintaining a controlled pool...

... TORC1 activation, recapitulates the phenotype caused by TSC1/2 disruption. Genetic studies suggest that TSC1/2 maintains ISCs independently of nutritional status or Notch regulation, probably by inhibiting cell delamination. We show that Tsc1 / Tsc2 mutant ISCs can efficiently produce enterocytes...

...Nikolaos Giagtzoglou; Tongchao Li; Shinya Yamamoto; Hugo J. Bellen Summary Notch signaling is an evolutionarily conserved pathway that plays a central role in numerous developmental and disease processes. The versatility of the Notch pathway relies on the activity of context-dependent regulators...

... endosomes contain Notch and other trans-membrane proteins as cargo. We report the first electron microscopy analysis of Dmon1 cells in a metazoan and extend the analysis of the endosomes in mutant cells. Our results suggest that the phenotype can be explained by the loss of function of Rab7. Moreover...

...Stéphanie Le Bras; Christine Rondanino; Géraldine Kriegel-Taki; Aurore Dussert; Roland Le Borgne Summary Notch signalling is involved in numerous cellular processes during development and throughout adult life. Although ligands and receptors are largely expressed in the whole organism, activation...

... resulted in a disordered alignment of photoreceptor neurons in larval stage and roughened eyes with aberrant ommatidia in adult flies. Furthermore, AP-1-depleted photoreceptor neurons showed an intracellular accumulation of a Notch regulator, Scabrous, and downregulation of Notch by promoting its...

...Elena Domanitskaya; Trudi Schüpbach The Notch signaling pathway plays important roles in a variety of developmental events. The context-dependent activities of positive and negative modulators dramatically increase the diversity of cellular responses to Notch signaling. In a screen for mutations...

...Youping Sun; Malgorzata Klauzinska; Robert J. Lake; Joseph M. Lee; Stefania Santopietro; Ahmed Raafat; David Salomon; Robert Callahan; Spyros Artavanis-Tsakonas Notch receptors and their ligands have crucial roles in development and tumorigenesis. We present evidence demonstrating the existence...