... immune cell response by co-culturing infected organoids with neutrophils, and determined that effectors like CPAF (also known as CT858) and TepP (also known as CT875) limit the recruitment of neutrophils to infected organoids. Collectively, our model can be applied to study the cell biology of Chlamydia...

... that topical insulin application could reverse the impaired inflammation reaction in the diabetic wound environment and promote healing of diabetic wounds. Our study revealed that insulin promoted apoptosis of neutrophils and subsequently triggered polarization of macrophages. Both in vivo and in vitro studies...

...Taylor J. Schoen; Emily E. Rosowski; Benjamin P. Knox; David Bennin; Nancy P. Keller; Anna Huttenlocher; Ana-Maria Lennon-Duménil ABSTRACT Neutrophils are primary phagocytes of the innate immune system that generate reactive oxygen species (ROS) and mediate host defense. Deficient phagocyte NADPH...

...Bhagawat C. Subramanian; Konstadinos Moissoglu; Carole A. Parent ABSTRACT Neutrophils sense and respond to diverse chemotactic cues through G-protein-coupled receptors (GPCRs). However, the precise trafficking dynamics of chemoattractant GPCRs during neutrophil activation and chemotaxis remain...

... with four members FAM3A, FAM3B, FAM3C and FAM3D. In this study, we found that FAM3D strongly chemoattracted human peripheral blood neutrophils and monocytes. To identify the FAM3D receptor, we used chemotaxis, receptor internalization, Ca 2+ flux and radioligand-binding assays in FAM3D-stimulated HEK293...

...Sharon Dewitt; Robert J. Francis; Maurice B. Hallett Summary Following adherence of neutrophils to the endothelium, neutrophils undergo a major morphological change that is a necessary prelude to their extravasation. We show here that this shape change is triggered by an elevation of cytosolic...

... advantage of the genetic tractability and translucency of zebrafish larvae to first characterise how a null mutant in zfWASp influences the behaviour of neutrophils and macrophages in response to tissue damage and to clearance of infections. We then use this mutant background to study how leukocyte lineage...

...Emma Colucci-Guyon; Jean-Yves Tinevez; Stephen A. Renshaw; Philippe Herbomel The early control of potentially invading microbes by our immune system primarily depends on its main professional phagocytes – macrophages and neutrophils. Although the different functions of these two cell types have...

... described in all cell types of the immune system, with the exception of neutrophils. In the present work we report for the first time the expression in human neutrophils of NFAT2 mRNA and protein. We also report that specific antigens were able to promote NFAT2 protein translocation to the nucleus...

... [PtdIns(3,4,5) P 3 ] production. Here we report that in neutrophilic HL-60 cells expressing PH-Akt-GFP, binding of iC3b-coated zymosan particles (2 μm in diameter) via β2 integrin induces an incomplete phagocytic cup to form before either PtdIns(3,4,5) P 3 or phosphatidylinositol (3,4) bisphosphate...

... in leukocyte migration through this barrier. In this context, blockade of α6 integrins (laminin receptors), neutrophil elastase (NE) or both inhibited neutrophil migration through interleukin-1β (IL-1β)-stimulated mouse cremasteric venules, as observed by intravital microscopy. Furthermore, analysis of tissues...

.... The subfamily of PH-domain-containing proteins described above are considered to be reporters for sites of PtdIns(3,4,5) P 3 /PtdIns(3,4) P 2 accumulation. Spatio-temporal localization studies suggest that a directional stimulation through chemoattractant receptors (GPCRs in Dictyostelium and neutrophils...

...Sharon Dewitt; Iraj Laffafian; Maurice B. Hallett The temporal and spatial relationship between particle binding to the neutrophil by β2 integrin (CR3), the Ca 2+ elevation and subsequent oxidase activation has been unclear. This is because of the difficulty in studying the time course...

...Sten Theander; Daniel P. Lew; Oliver Nüße Ca 2+ -induced exocytosis in neuronal and neuroendocrine cells involves ATP-dependent steps believed to `prime' vesicles for exocytosis. Primed, docked vesicles are released in response to Ca 2+ influx through voltage-gated Ca 2+ channels. Neutrophils...

... and migration across the endothelium. A number of factors govern the interactions between leuko- cytes and endothelial cells, including the expression of mol- ecules on the surface of activated leukocytes and/or endo- thelial cells, certain products of neutrophil activation, and shear forces within blood...