...Connie Shen; Eva Mulder; Wiebe Buitenwerf; Jérémy Postat; Aron Jansen; Matthijs Kox; Judith N. Mandl; Nienke Vrisekoop ABSTRACT Neutrophils are among the fastest-moving immune cells. Their speed is critical to their function as ‘first responder’ cells at sites of damage or infection, and it has...

... were significantly less efficient at bacterial uptake and killing compared to monocytes, and this activity was associated with a reduced inflammatory cytokine response. The presence of bacteria triggered the release of DNA (eDNA) from LDGs into the extracellular space that resembled neutrophil...

...- and C-termini, accompanies Ca 2+ -induced cell surface expansion. Ezrin Phagocytosis Cell spreading Myeloid cell Neutrophil Ca 2+ signalling Medical Research Council 10.13039/501100000265 Cardiff University 10.13039/501100000866 Two important cell biological...

... a more mesenchymal mode of migration. Here, we sought to characterize the mechanisms that regulate neutrophil and macrophage migration in vivo by using real-time imaging of leukocyte motility within interstitial tissues in zebrafish larvae. Neutrophils displayed a rounded morphology and rapid protease...

... microglia. Moreover, both macrophages and neutrophils of Trim33-deficient embryos display a reduced basal mobility within interstitial tissues, and a profound lack of a response to inflammatory recruitment signals, including local bacterial infections. Correlatively, Trim33-deficient mouse bone marrow...

...Rohola Hosseini; Gerda E. M. Lamers; Hiwa M. Soltani; Annemarie H. Meijer; Herman P. Spaink; Marcel J. M. Schaaf ABSTRACT Macrophages and neutrophils are the first responders to invading pathogens and contribute strongly to the host defense against intracellular pathogens. The collective interplay...

...Kun Gao; Wenwen Tang; Yuan Li; Pingzhao Zhang; Dejie Wang; Long Yu; Chenji Wang; Dianqing Wu ABSTRACT A hallmark of neutrophil polarization is the back localization of active RHOA and phosphorylated myosin light chain (pMLC, also known as MYL2). However, the mechanism for the polarization...

... been attributed to its GEF activity towards Rac1, we show that P-Rex1 also acts as a GEF for the Rac-related GTPase RhoG, both in vitro and in GPCR-stimulated primary mouse neutrophils. Furthermore, loss of either P-Rex1 or RhoG caused equivalent reductions in GPCR-driven Rac activation and Rac...

...Krishma Halai; James Whiteford; Bin Ma; Sussan Nourshargh; Abigail Woodfin ABSTRACT Intercellular adhesion molecule 2 (ICAM-2) is expressed on endothelial cells (ECs) and supports neutrophil extravasation. However, the full details of its role remain unknown, and the present study investigates...

... in living organisms. Here we exploited the zebrafish system to study the role of microtubules in neutrophil migration in vivo . The localization of microtubules was visualized in motile neutrophils using various bioprobes, revealing that, in contrast to what has been seen in studies in vitro...

...Pui-ying Lam; Sa Kan Yoo; Julie M. Green; Anna Huttenlocher Summary Neutrophil recruitment to sites of injury or infection is essential for host defense, but it needs to be tightly regulated to prevent tissue damage. Phosphoinositide 3-kinase (PI3K), which generates the phosphatidylinositol (3,4,5...

... is altered in disease. * Author for correspondence ( huttenlocher@wisc.edu ) ‡Present address: 4205 Microbial Sciences Building, 1550 Linden Drive, Madison, WI 53706, USA © 2012. Published by The Company of Biologists Ltd 2012 Chemotaxis Migration Neutrophil Zebrafish...

...Rajesh K. Singh; Wenjia Liao; Dhani Tracey-White; Chiara Recchi; Tanya Tolmachova; Sara M. Rankin; Alistair N. Hume; Miguel C. Seabra Neutrophil migration is vital for immunity and precedes effector functions such as pathogen killing. Here, we report that this process is regulated by the Rab27a...

... by phospholipase D (PLD) enzymes. Neutrophils, important cells of the innate immune system, maintain the body's defence against infection. Previous studies have implicated PLD-generated PA in neutrophil function; these have relied heavily on the use of primary alcohols to act as inhibitors of PA production...

...Yasuhito Hayashi; Mindy K. Call; Tai-ichiro Chikama; Hongshan Liu; Eric C. Carlson; Yan Sun; Eric Pearlman; James L. Funderburgh; George Babcock; Chia-Yang Liu; Yuichi Ohashi; Winston W.-Y. Kao An important aspect of wound healing is the recruitment of neutrophils to the site of infection or tissue...

... as Was) on leukocyte migration in response to a wound. In embryos at 22 hours post-fertilisation, primitive macrophages were impaired in their migration towards laser wounds. Once a circulatory system had developed, at 3 days post-fertilisation, we observed significantly reduced recruitment of neutrophils...

... the role of PI3K in mediating chemotaxis to fMLP, both in vitro and in vivo. Using short- and long-term in vitro assays, as well as an in vivo chemotaxis assay, we investigated the importance of PI3K in response to the prototypic chemoattractant fMLP. Exposure of neutrophils to fMLP induced an immediate...

... identified in an insertional mutagenesis screen for mutants that exhibit abnormal tissue distribution of neutrophils. We identified a mutant line with an insertion in the Hepatocyte growth factor activator inhibitor 1 gene ( hai1 ; also known as Spint1 ) that showed accumulation of neutrophils in the fin...

..., but to inhibited cell migration. Although undetected in cells of non-migratory tissues as well as in confluent cultured cells, S1 fibers were found in all migratory cells examined, such as cultured cells in scratch/wound experiments, blood neutrophils and monocytes, and fibroblasts engaging in tissue healing...

...Marc Herant; Volkmar Heinrich; Micah Dembo The mechanical implementation of phagocytosis requires a well-coordinated deployment of cytoplasm and membrane during the creation of a phagosome. We follow the time course of this process in initially round passive neutrophils presented with antibody...