... analysed the interaction of mitochondria with microtubules in the filamentous fungus Neurospora crassa . Mitochondria were fluorescently labelled by expression of matrix-targeted green fluorescent protein. Upon isolation, mitochondria collapsed to round spherical structures that were still able to interact...

...: vegetative hyphae of Neurospora crassa ; pollen tubes of Agapanthus umbellatus ; and rhizoids of Dryopteris affinis gametophytes. Examination of cyto-plasmic pH in growing cells was performed by simultaneous, dual emission confocal ratio imaging of the pHsensitive probe carboxy SNARF-1. Considerable...