... of cells and their fate during embryonic development remains largely unknown. Here, we investigated the role of glucose metabolism in the development of avian trunk neural crest cells (NCCs), a migratory stem cell population of the vertebrate embryo. We uncovered that trunk NCCs display glucose oxidation...

... exosome cell–cell communication. Using chick cranial neural fold cultures, we show that migratory neural crest cells, a developmentally critical cell type and model for metastasis, release and deposit CD63-positive 30–100 nm particles into the extracellular environment. Neural crest cells contain ceramide...

...Karyn Jourdeuil; Lisa A. Taneyhill ABSTRACT Gap junctions are intercellular channels between cells that facilitate cell–cell communication. Connexin 43 (Cx43; also known as GJA1), the predominant gap junction protein in vertebrates, is expressed in premigratory cranial neural crest cells...

...Rangarajan Padmanabhan; Lisa A. Taneyhill The epithelial‐to‐mesenchymal transition (EMT) is important for the formation of migratory neural crest cells during development and is co‐opted in human diseases such as cancer metastasis. Chick premigratory cranial neural crest cells lose intercellular...

...Genevieve Abbruzzese; Anne-Kathrin Gorny; Lilian T. Kaufmann; Hélène Cousin; Iivari Kleino; Herbert Steinbeisser; Dominique Alfandari ABSTRACT Cranial neural crest (CNC) cells are a transient population of stem cells that originate at the border of the neural plate and the epidermis, and migrate...

... of any of the binding sites in the Rac1–RCC2–Coro1C complex delays the arrival of neural crest derivatives at the correct location in developing zebrafish, demonstrating the crucial role in migration guidance in vivo . References Bass   M. D. , Roach   K. A. , Morgan   M. R. , Mostafavi...

...Parinya Noisa; Carina Lund; Kartiek Kanduri; Riikka Lund; Harri Lähdesmäki; Riitta Lahesmaa; Karolina Lundin; Hataiwan Chokechuwattanalert; Timo Otonkoski; Timo Tuuri; Taneli Raivio ABSTRACT Neural crest cells are specified at the border between the neural plate and the epiderm. They are capable...

... cranial neural crest EMT. Tspan18 mRNA is expressed in premigratory cranial neural crest cells, but is absent from actively migrating neural crest cells. Tspan18 knockdown leads to a concomitant loss of cadherin-6B (Cad6B) protein, whereas Cad6B protein persists when Tspan18 expression is extended...

...Yujin Zhang; Winston W.-Y. Kao; Emanuele Pelosi; David Schlessinger; Chia-Yang Liu Notch signaling is pivotal for the morphogenesis and homeostasis of many tissues. We found that aberrant Notch activation in mouse neural-crest-derived periocular mesenchymal cells (POMCs), which contribute...

... at sites of intercellular contact during several developmental migrations. For example, the expression of ephrin-Bs in posterior halves of somites restricts neural crest cell migration to the anterior halves. Here we show that ephrin-B2 destabilises neural crest cell lamellipodia when presented...

... potential, neural crest cells constitute a powerful paradigm to address this question. Here, we describe that laminin-1, a major component of their migration routes, promotes crest cell spreading, migration and survival through two distinct integrin-binding domains that are situated on both sides of its α1...

...Sandrine Testaz; Muriel Delannet; Jean-Loup Duband ABSTRACT Based on genetic, functional and histological studies, the extracellular matrix molecule fibronectin has been proposed to play a key role in the migration of neural crest cells in the vertebrate embryo. In the present study, we have...

...Nathalie Desban; Jean-Loup Duband ABSTRACT In the present study, to further elucidate the molecular events that control neural crest cell migration, we have analyzed in vitro the adhesive and locomotory response of avian trunk neural crest cells to laminin-1 and searched for the integrin receptors...

...Joanne G. Stone; Lucy I. Spirling; Michael K. Richardson ABSTRACT The peptide endothelin 3 (EDN3) is essential for normal neural crest development in vivo, and is a potent mitogen for quail truncal crest cells in vitro. It is not known which subpopulations of crest cells are targets...

...Frédérique Monier-Gavelle; Jean-Loup Duband ABSTRACT Dispersion of neural crest cells and their ultimate regroup-ment into peripheral ganglia are associated with precisely coordinated regulations both in time and space of the expression and function of cell adhesion receptors. In par-ticular...

...Linda A. Thomas; Kenneth M. Yamada ABSTRACT Mass migrations of dense cell populations occur periodically during embryonic development. It is known that extracellular matrices, through which the cells migrate, facilitate locomotion. However, this does not explain how cells, such as neural crest, can...

...Anne Baroffio; Michel Blot ABSTRACT The neural crest (NC) of vertebrate embryos yields cell types belonging to the neural, melanocytic and mesec-todermal lineages. To test the possibility that the precursors of these lineages segregate from pluripotent cells by a process involving stochastic...

...E. J. Sanders; E. Cheung ABSTRACT The sclerotome portion of the differentiating embryonic chick somite becomes infiltrated by neural crest cells prior to its dispersal towards the embryonic axis. This means that sclerotome cells explanted into culture for the purpose of examining their interactions...