.... ‡ Authors for correspondence (e-mail: gerald.hammond@cancer.org.uk ; giampietro.schiavo@cancer.org.uk ) * Present address: Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1PD, UK Diacylglycerol Exocytosis Neomycin Phosphoinositide Phospholipase C...

... was lost after mutation of potential PtdIns(4,5) P 2 -binding motifs. Moreover, neomycin, which masks PtdIns(4,5) P 2 polar heads, was shown to decrease by 50% the association of PTPL1 with the cytoskeletal fraction. These results identify the crucial role of the FERM domain in PTPL1 intracellular...

... with epidermal growth factor. In certain types of kidney-derived cells such as MDCK cells, however, ERM proteins appear to be activated in the absence of Rho activation and remain active without C-terminal phosphorylation. Interestingly, microinjection of an aminoglycoside antibiotic, neomycin, which binds...

...PAUL M. LARSEN; TUNG-LING L. CHEN; STEPHEN M. WOLNIAK Neomycin has been reported to inhibit polyphosphoinositide cycling by preventing the hydrolysis of phosphatidylinositol 4,5-bisphosphate into inositol 1,4,5-trisphosphate and 1,2-diacylglycerol. Inositol 1,4,5-trisphosphate, through...