... reported that nerve growth factor (NGF)-induced MAPK activation in a PC12-derived cell line was potentiated by tau, with phosphorylation at T231 being required. Therefore, we sought to identify a signaling molecule involved in the NGF-induced Ras-MAPK pathway that interacted with phospho-T231-tau. Here, we...

... (NGF) acting through the TrkA neurotrophin receptor (also known as NTRK1) regulates many of these events. However, the molecular mechanisms responsible for NGF-regulated secretion are not completely understood. Here, we describe a new signaling pathway involving TrkA, ARMS (also known as Kidins220...

... localization of GluA2 (also known as GRIA2), an AMPA receptor subunit. Here, we investigated a possible interaction between retrograde signaling pathways for Sema3A and nerve growth factor (NGF). Sema3A induces colocalization of PlexA4 (also known as PLXNA4) signals with those of tropomyosin-related kinase...

... in each experiment. In the case of treatment with GM6001, BB94 and compound XXI, cells were treated with the different compounds for 1 hour prior to fixation and staining. For NGF or BDNF, cells were serum starved for 2 hours then treated with the different neurotrophins (25 ng/ml) for 1 hour or 24...

... steps of ligand-induced TrkA responses at the cell plasma membrane is missing. We have exploited single particle tracking and TIRF microscopy to study TrkA membrane lateral mobility and changes of oligomerization state upon binding of diverse TrkA agonists (NGF, NGF R100E HSANV mutant, proNGF and NT-3...

... directly. We show here that NGF- and EGF-induced initial cell extensions result in APC phosphorylation by the MAPK/ERK pathway, which, in parallel with inhibition of GSK-3β, promotes localization of APC to the tip of cell extensions. Whereas GSK-3β inhibition promotes APC binding and stabilization...

... cleavage and Trk signaling. We found that, in PC12 cells, nerve growth factor (NGF) induces rapid and robust α-secretase- and γ-secretase-dependent cleavage of p75NTR, releasing the resulting intracellular domain into the cytosol. Brain-derived neurotrophic factor similarly induces p75NTR cleavage...

... we investigated the expression of GSK3β and its activity towards MAP1B during nerve growth factor (NGF)-stimulated PC12 cell differentiation. Levels of GSK3β expression increase relatively little during the course of differentiation. However, the expression of a novel GSK3β isoform characterised...

...Ok-Sun Bang; Eui Kyun Park; Sung-Il Yang; Sun-Ryung Lee; Thomas F. Franke; Shin-Sung Kang ABSTRACT To investigate the role of Akt in nerve growth factor (NGF)-induced neuronal differentiation, PC12 cells ectopically expressing wild-type or dominant-inhibitory forms of Akt were analyzed. NGF-induced...

... tracking to monitor the effects of nerve growth factor (NGF) on the movements of β1 integrin in the plane of the plasma membrane of the filopodia of growth cones. β1 integrin was visualized by its binding of 0.2 µm beads coated with a monoclonal Ab directed against an extracellular epitope distant from...

... of neural tissue. To investigate the role of goosecoid in neuronal differentiation of mammalian cells, we have introduced goosecoid into PC12 cells. Expression of goosecoid resulted in reduced cell proliferation and enhanced neurite outgrowth in response to NGF. Expression of goosecoid led to a decrease...

... is thought to mediate loss of intercellular adhesion promoted by activation of receptor tyrosine kinases in epithelial cells. Here, we show that activation of ectopically expressed TrkA, the tyrosine kinase receptor for nerve growth factor (NGF), stimulates embryonal carcinoma P19 cells to develop extensive...

.... Furthermore, NGF increases c-Jun phosphorylation in parallel with the induction of apoptosis in cultures of explanted otic vesicles, and this increase is inhibited by IGF-I or viral-mediated overexpression of Raf. These results suggest that Jun transcription factors may specify proliferative and apoptotic...

... by Company of Biologists 1998 Apoptosis Programmed cell death tau1 PHF1 Neuron PC12 NGF Protein phosphatase 2A Okadaic acid Microtubule Acetylated tubulin Alzheimer’s disease Apoptosis can be divided into three sequential phases ( Jacobson et al., 1994 ; Greenlund et al., 1995...

... of secretion, epithelial transport, exocytosis and endocytosis as well as the regulation of mouse SMG specific gene expression, in particular, NGF, EGF and renin. The postnatal development and sexual dimorphism of the mouse gland permits the isolation of male SMGs of different ages, corresponding to different...

...Babak Esmaeli-Azad; Joseph H. McCarty; Stuart C. Feinstein ABSTRACT Microtubules are fundamental elements participating in many aspects of cell behavior and maintenance, yet the factors regulating microtubule behavior in vivo remain poorly understood. Employing the nerve growth factor (NGF...

...Curt M. Horvath; Amy Wolven; Debbie Machadeo; Julie Huber; Lana Boter; Marta Benedetti; Barbara Hempstead; Moses V. Chao ABSTRACT Nerve growth factor (NGF) represents a family of structurally related trophic factors, including brain-derived neurotrophin factor (BDNF), neurotrophin-3 (NT-3), NT-4...

... and maturation) and oligodendrocytes (possibly proliferation, inhibition of maturation) and further depend upon the site of origin of the cells. With regard to glial cells, bFGF therefore differs from NGF, which does not influence glial cell morphology and physiology. To our knowledge, there is only one...