... to the nuclear retinoic acid receptor (RAR) and whose mobilization to the nucleus is mediated by a ‘classical-like’ nuclear localization signal (NLS). HuR is also required for heregulin-induced nuclear translocation of the NFκB subunit p65, which contains both classical and non-canonical NLSs. HuR thus regulates...

...Sarah McKenna; Clyde J. Wright ABSTRACT Multiple mediators of septic shock are regulated by the transcription factor nuclear factor κB (NFκB). However, complete NFκB inhibition can exacerbate disease, necessitating evaluation of targeted strategies to attenuate the pro-inflammatory response. Here...

... expression profiles, impaired the TCR-induced Ca 2+ flux and nuclear expression of NFAT2, suppressed upregulation of molecules essential for activation, and attenuated signalling pathways upstream of the AP-1 and NFκB complexes, leading to reduced activation of these important transcription factors...

... progression. Despite the importance of the SASP to tumor biology, its regulation remains relatively unknown. Here, we show that IκBζ, an atypical member of the inhibitor of NFκB proteins and selective coactivator of particular NFκB target genes, is an important regulator of SASP expression. Several models...

...Ying Sun; Yuanyuan Duan; Anna S. Eisenstein; Wenbao Hu; Adrien Quintana; Wai Kwan Lam; Yan Wang; Zhenguo Wu; Katya Ravid; Pingbo Huang Summary The nuclear factor kappa B (NFκB) pathway controls a variety of processes, including inflammation, and thus, the regulation of NFκB has been a continued...

..., independently of NFκB. IKKα regulates canonical TGFβ–SMAD signaling by interacting with SMAD3 and controlling SMAD complex formation on DNA. Furthermore, we demonstrate that the TGFβ–IKKα–SMAD signaling pathway induces transcription of the genes encoding SNAIL and SLUG. In addition, we demonstrate that IKKα...

...Gabrielle Carvalho; Armelle Le Guelte; Catherine Demian; Aimé Vazquez; Julie Gavard; Nicolas Bidère T-cell-receptor (TCR) signalling to NFκB requires the assembly of a large multiprotein complex containing the serine/threonine kinase CK1α, the scaffold protein CARMA1, the heterodimer BCL10-MALT1...

... containing protein)-like receptors (NLRs) have been implicated as phylogenetically ancient sensors of intracellular pathogens or endogenous danger signals. NOD2 recognizes the bacterial cell wall component muramyldipeptide leading to NFκB and MAPK activation via induced proximity signalling through...

...Cristina Aguilera; Vanessa Fernández-Majada; Julia Inglés-Esteve; Verónica Rodilla; Anna Bigas; Lluís Espinosa IκB are responsible for maintaining p65 in the cytoplasm under non-stimulating conditions and promoting the active export of p65 from the nucleus following NFκB activation to terminate...

... NFκB. After coculturing, protein was isolated from cell cultures using TRI®reagent (Sigma, St Louis, MO). Protein was separated from RNA and DNA by chloroform and ethanol extraction and precipitated using isopropanol. Protein pellets were washed with guanidinium chloride/95% ethanol and dissolved...

...Lluís Espinosa; Sara Santos; Julia Inglés-Esteve; Pura Muñoz-Canoves; Anna Bigas Notch/RBP-Jκ and nuclear factor-κB (NFκB) complexes are key mediators of the progression of many cellular events through the activation of specific target gene transcription. Independent observations have shown...