... of attracting monocytes. Additionally, we showed that Nef-induced CCL2/MCP-1 expression depends on the myristoylation moiety of Nef and requires functional calmodulin. In summary, we suggest that Nef-induced CCL2/MCP-1 expression in astrocytes contributes to infiltration of monocytes into the brain, and thereby...

... that the NCS protein VILIP-1 influences the activity of the receptor guanylyl cyclase GC-B. In transfected cell lines, VILIP-1 performs a Ca 2+ -dependent membrane association, the reversible Ca 2+ -myristoyl switch of VILIP-1, which leads to an increase in natriuretic peptide-stimulated cGMP levels...

...Susanne Bolte; Spencer Brown; Béatrice Satiat-Jeunemaitre We report on the sub-cellular localisation and function of m-Rab mc , a N-myristoylated plant-specific Rab-GTPase previously characterised at the molecular level and also by structural analysis in Mesembryanthemum crystallinum . By confocal...

... signal. When expressed in COS-7 cells, mutant hARL5(T35N), which is predicted to be GDP bound, was concentrated in nucleoli. The N-terminus of hARL5, like that of ARF, was myristoylated. Yeast two-hybrid screening and in vitro protein-interaction assays showed that hARL5(Q80L),predicted to be GTP bound...

... product. PSD-Zip70 contains an N-myristoylation consensus sequence, a polybasic cluster in the N-terminal region and four leucine-zipper motifs in the C-terminal region. Light and electron microscopy showed that this protein was localized to the dendritic spines, especially in the PSD and the postsynaptic...

...Inmaculada Navarro-Lérida; Alberto Álvarez-Barrientos; Francisco Gavilanes; Ignacio Rodriguez-Crespo Using recursive PCR, we created an artificial protein sequence that consists of a consensus myristoylation motif (MGCTLS) followed by the triplet AGS repeated nine times and fused to the GFP...

...Sang Soo Kim; Jung Hwa Kim; Seung-Hye Lee; Sung Soo Chung; Ok-Sun Bang; Dongeun Park; Chin Ha Chung Myristoylated alanine-rich C kinase substrate (MARCKS) translocates from the cytosol to the plasma membrane while mononucleated myoblasts fuse to form multinucleated myotubes. Here, we show...

... A. By mass spectrometry, GAPR-1 was shown to be myristoylated. Immunoprecipitation of GAPR- 1 from Golgi membranes resulted in the coimmunoprecipitation of caveolin-1, indicating a direct interaction between these two proteins. Myristoylation, together with protein-protein or electrostatic interactions...

...P. Zlatkine; B. Mehul; A. I. Magee ABSTRACT Several members of the Src family of protein tyrosine kinases have a N-terminal dual acylation motif which specifies their myristoylation and S-acylation. These lipid modifications are necessary for correct intracellular localisation to the plasma...

...Thérèse David-Pfeuty; Shubha Bagrodia; David Shalloway ABSTRACT Myristoylation of pp60 src is required for its membrane attachment and transforming activity. The mouse monoclonal antibody, mAb327, which recognizes both normal, myristoylated pp60 c-src and a nonmyristoylated mutant, pp60 c-src/myr...