... PDGFRα expression in FAPs, mouse dermal fibroblasts and in two related mesenchymal cell lines. Moreover, TGF-β promotes myofibroblast differentiation of FAPs but inhibits their adipogenicity. Accordingly, TGF-β impairs the expression of PDGFRα-dependent immediate early genes in a TGFBR1-dependent manner...

...Lisa Leinhos; Johannes Peters; Sabine Krull; Lena Helbig; Melanie Vogler; Magdolna Levay; Gijsbert J. van Belle; Anne J. Ridley; Susanne Lutz; Dörthe M. Katschinski; Anke Zieseniss ABSTRACT Fibroblasts show a high range of phenotypic plasticity, including transdifferentiation into myofibroblasts...

...Franco Klingberg; Grace Chau; Marielle Walraven; Stellar Boo; Anne Koehler; Melissa L. Chow; Abby L. Olsen; Michelle Im; Monika Lodyga; Rebecca G. Wells; Eric S. White; Boris Hinz ABSTRACT Dysregulated secretion and extracellular activation of TGF-β1 stimulates myofibroblasts to accumulate...

...Stephanie R. Gillespie; Liana J. Tedesco; Lingyan Wang; Audrey M. Bernstein ABSTRACT Scarring and fibrotic disease result from the persistence of myofibroblasts characterized by high surface expression of αv integrins and subsequent activation of the transforming growth factor β (TGFβ) proteins...

...Scott D. Varney; Courtney B. Betts; Rui Zheng; Lei Wu; Boris Hinz; Jiliang Zhou; Livingston Van De Water ABSTRACT How mechanical cues from the extracellular environment are translated biochemically to modulate the effects of TGF-β on myofibroblast differentiation remains a crucial area...

... and extracellular matrix composition. Cadherins mechanically link neighboring cells and are likely to contribute to fibrotic disease propagation. Finally, transition to the active myofibroblast phenotype leads to maladaptive tissue remodeling and enhanced mechanotransductive signaling, forming a positive feedback...

... −/− injured muscles. In vitro , Sharp-1 associates with Smad3, and its overexpression inhibits TGF-β- and Smad3-mediated expression of extracellular matrix genes in myofibroblasts. These results demonstrate that Sharp-1 regulates muscle regenerative capacity, at least in part, by modulation of TGF-β signaling...

... of periostin, day 7 wounds showed a significant reduction in myofibroblasts, as visualized by expression of α-smooth muscle actin (α-SMA) within the granulation tissue. Delivery of recombinant human periostin by electrospun collagen scaffolds restored α-SMA expression. Isolated wild-type and knockout dermal...

... due to a severe reduction in the number of myofibroblasts, whereas inflammatory infiltrate and vascularization of the granulation tissue are unaffected. Primary ILK-deficient fibroblasts exhibit severely reduced levels of extracellular TGFβ1, α-smooth muscle actin (αSMA) production and myofibroblast...

... actin (α-SMA). Integrin-β1-deficient fibroblasts showed reduced expression of type I collagen and connective tissue growth factor, and failed to differentiate into myofibroblasts as a result of reduced α-SMA stress fiber formation. Loss of integrin β1 in adult fibroblasts reduced their ability to adhere...

...Lysianne Follonier Castella; Lara Buscemi; Charles Godbout; Jean-Jacques Meister; Boris Hinz Myofibroblasts promote tissue contractures during fibrotic diseases. To understand how spontaneous changes in the intracellular calcium concentration, [Ca 2+ ] i , contribute to myofibroblast contraction...

... of tissue under remodeling. * Author for correspondence (e-mail: [email protected] ) 31 12 2007 © The Company of Biologists Limited 2008 2008 Fig. 8. Possible models for AJ reinforcement in differentiated myofibroblasts. In our experiments, the reinforcement of OB-cadherin bonds...

.... e-mails: [email protected] ; [email protected] 21 8 2007 © The Company of Biologists Limited 2007 2007 Stress fibre Stress fibre Rho GTPase Actin Contractility Cell migration Myofibroblast Myoepithelial We have known for over 50 years...

...Bahar Cat; Dominik Stuhlmann; Holger Steinbrenner; Lirija Alili; Olaf Holtkötter; Helmut Sies; Peter Brenneisen Myofibroblasts, pivotal for tumor progression, populate the microecosystem of reactive stroma. Using an in vitro tumor-stroma model of skin carcinogenesis, we report here that tumor-cell...

... appearance of myofibroblasts at the wound site. Wounds made to vimentindeficient embryos also failed to heal during the 24 hour culture period it takes for wildtype embryos to fully heal an equivalent wound. By DiI marking the wound mesenchyme and following its fate during the healing process we showed...

.... The myofibroblast cell line BHK-21/C13 was described to assemble myofibrils in culture. We have now characterized the sub-line BHK-21-Bi, which bears a small deletion within the titin gene. RNA analysis revealed that in this mutant cell line only a small internal portion of the titin mRNA is deleted. However...