...Yen-Ling Lian; Kuan-Wei Chen; Yu-Ting Chou; Ting-Ling Ke; Bi-Chang Chen; Yu-Chun Lin; Linyi Chen ABSTRACT Myoblast fusion is required for myotube formation during myogenesis, and defects in myoblast differentiation and fusion have been implicated in a number of diseases, including human...

... and fusion pore formation, ultimately resulting in the integration of two fusion partners. Here, we focus on the asymmetric actin cytoskeletal rearrangements at the site of fusion, known as the fusogenic synapse, which was first discovered during myoblast fusion in Drosophila embryos and later also found...

... several proteins involved in myoblast fusion have been identified, knowledge about their subcellular regulation is rather elusive. We identified the anaphase-promoting complex (APC/C) adaptor Fizzy related (Fzr) as an essential regulator of heart and muscle development. We show that APC/C Fzr regulates...

...Julia Hamp; Andreas Löwer; Christine Dottermusch-Heidel; Lothar Beck; Bernard Moussian; Matthias Flötenmeyer; Susanne-Filiz Önel ABSTRACT The fusion of founder cells and fusion-competent myoblasts (FCMs) is crucial for muscle formation in Drosophila . Characteristic events of myoblast fusion...

... revealed an important function of WHAMY in membrane protrusions and cell migration in macrophages. Genetic data further implied synergistic functions between WHAMY and WASP during morphogenesis. Double mutants were late-embryonic lethal and showed severe defects in myoblast fusion. Trans-heterozygous...

... muscle fibres arise by fusion of precursor cells called myoblasts. We previously showed that CKIP-1 ectopic expression in C2C12 myoblasts increased cell fusion. In this work, we report that CKIP-1 depletion drastically impairs C2C12 myoblast fusion in vitro and in vivo during zebrafish muscle development...

...Scott J. Nowak; Patrick C. Nahirney; Anna-Katerina Hadjantonakis; Mary K. Baylies Myoblast fusion is crucial for the formation, growth, maintenance and regeneration of healthy skeletal muscle. Unfortunately, the molecular machinery, cell behaviors, and membrane and cytoskeletal remodeling events...

... myoblasts even when grown under differentiation conditions. Specifically, removal of GPI-anchored proteins from the surface of C2C12 myoblasts resulted in a decrease in myoblast fusion index (0.08±0.07 versus 0.65±0.12; P =0.004) and an increased level of BrdU incorporation (37±2% versus 3±1% BrdU-positive...

..., a mutation preventing MARCKS phosphorylation and thus facilitating MARCKS translocation resulted in promotion of the cell fusion. In contrast, overexpression of MARCKS carrying a mutation that blocks myristoylation and thus prevents the MARCKS translocation impaired the myoblast fusion. Together...