... of muscle stem cells and their progeny is crucial in strategizing remedies for pathological dysregulation of these cues in aging and disease. In this study, we investigated the cell-level influences of extracellular matrix (ECM) ligands and culture substrate stiffness on primary human myoblast contractility...

...Hardik P. Gala; Debarya Saha; Nisha Venugopal; Ajoy Aloysius; Gunjan Purohit; Jyotsna Dhawan ABSTRACT Adult stem cells persist in mammalian tissues by entering a state of reversible quiescence, referred to as G 0 , which is associated with low levels of transcription. Using cultured myoblasts...

...Julie Perroud; Laurent Bernheim; Maud Frieden; Stephane Koenig ABSTRACT Ca 2+ signaling plays a key role during human myoblast differentiation. Among Ca 2+ -sensitive pathways, calcineurin is essential for myoblast differentiation and muscle regeneration. Nuclear factor of activated T-cell (NFAT...

... in pigs. In this study, we investigated the roles of these two miRNAs in myogenesis and analyzed their transcriptional regulation. Our results showed that miR-195 and miR-497 were upregulated during muscle development and myoblast differentiation. Moreover, miR-195 and miR-497 inhibited proliferation...

... ) 1 6 2010 © 2010. 2010 Fusion Myoblast Myocyte CXCR4 SDF-1α Regeneration Skeletal muscle degeneration can occur as a result of disease or injury; however, this tissue has an extensive ability to regenerate. Adult regenerative myogenesis is dependent on progenitor cells...

..., during which it mediates myoblast fusion. We isolated both damaged and intact myofibers from a mouse model of muscular dystrophy using laser-capture microdissection and found that the levels of myoferlin mRNA and protein were increased in damaged myofibers. To better define the components of the muscle...

... of Biologists Limited 2009 2009 Satellite cell Myoblast Presenilin-1 Id1 Pax7 MyoD γ-secretase Self-renewal Skeletal muscle Myogenic differentiation Stem cell Cell fate choice Muscle satellite cells are myogenic stem cells that are located between the basal lamina and the plasmalemma...

... to regenerate lost myofibers. Using synchronized cultures of C2C12 myoblasts to model these divergent programs, we show that p8 (also known as Nupr1 ), a G1-induced gene, negatively regulates the cell cycle and promotes myogenic differentiation. p8 is a small chromatin protein related to the high mobility group...

...Magali Louis; Nadège Zanou; Monique Van Schoor; Philippe Gailly Myoblast migration is a key step in myogenesis and regeneration. It allows myoblast alignment and their fusion into myotubes. The process has been shown to involve m-calpain or μ-calpain, two Ca 2+ -dependent cysteine proteases. Here...

...Massimiliano Cerletti; Michael J. Molloy; Kinga K. Tomczak; Soonsang Yoon; Marco F. Ramoni; Alvin T. Kho; Alan H. Beggs; Emanuela Gussoni Myoblast fusion is a highly regulated process that is important during muscle development and myofiber repair and is also likely to play a key role...

... dystrophy myoblasts: implications for cell and gene therapy. Somatic Cell Mol. Genet. 16 , 557 -565. Yablonka-Reuveni, Z., Rudnicki, M. A., Rivera, A. J., Primig, M., Anderson, J. E. and Natanson, P. ( 1999a ). The transition from proliferation to differentiation is delayed in satellite cells from...

... multiple biological responses. With the aim of extending our knowledge on the role played by S1P in the regulation of cytoskeletal reorganization, native as well as C2C12 myoblasts stably transfected with green fluorescent protein (GFP)-tagged α- and β-actin constructs were stimulated with S1P (1 μM...

... rely on the highly regulated spatiotemporal distribution of cell surface heparan sulfate proteoglycans to which are associated growth factors, thus creating a specific microenvironment around muscle cells. Most growth factors involved in control of myoblast growth and differentiation are stored...

..., the involvement of tyrosine phosphatases in this process is unknown. SHP-2 is an SH2 domain-containing tyrosine phosphatase, which positively regulates growth and differentiation. We show that in C2C12 myoblasts, SHP-2 becomes upregulated early on during myogenesis and associates with a 120 kDa tyrosyl...

... the receptor. Activators of PAR-2 include trypsin and mast cell tryptase. Skeletal myoblasts are known to express PAR-1, a thrombin receptor. The current study was undertaken to determine whether myoblasts express PAR-2. Primary neonatal rat and mouse skeletal myoblast cultures were shown to express PAR-2...

... load, there is little knowledge of how muscle cells are initially determined in the early embryo. In the zebrafish, fibres of the slow twitch class arise from precociously specified myoblasts that lie close to the midline whereas the remainder of the myotome differentiates as fast myosin expressing...

... time that prosomes are also genuine constituents of the nuclear matrix, chromatin and the nuclear RNP networks. Using mouse myoblasts we tested three monoclonal antibodies recognising the prosomal subunits p23K, p27K and p30K, and found that the corresponding prosome subclasses are characterised...

...Hyun J. Ra; Catherine Picart; Huisheng Feng; H. Lee Sweeney; Dennis E. Discher ABSTRACT To quantitatively elucidate attributes of myocyte-matrix adhesion, muscle cells were controllably peeled from narrow strips of collagen-coated glass. Initial growth of primary quail myoblasts on collagen strips...

...F. Edom-Vovard; V. Mouly; J. P. Barbet; G. S. Butler-Browne ABSTRACT To understand how and when myogenic precursor cells become committed to their particular developmental programs, we have analysed the different populations of myoblasts which grow out from explants of muscle tissue isolated from...

...A. Irintchev; J. D. Rosenblatt; M. J. Cullen; M. Zweyer; A. Wernig ABSTRACT We investigated the potential of cultured myoblasts to generate skeletal muscle in an ectopic site. Myoblasts from a clonal cell line or from expanded primary cultures were injected under the skin of the lumbar region...