... by weakening the lipid bilayer. Endocytosis Multivesicular body Intraluminal vesicles Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659 KO 963/8-1 Endosomal sorting complex required for transport (ESCRT)-III proteins are membrane remodeling factors...

...) and is spatially and temporally associated with tracheal tube fusion (anastomosis) events. The formation of Stac-positive luminal EVs depends on the tracheal tip-cell-specific GTPase Arl3 (also known as Dnd in Drosophila ), which is also required for the formation of Stac-positive multivesicular bodies (MVBs...

... the multivesicular body (MVB). Completion of MVB sorting is accompanied by maturation of the endosome into a late endosome, an event that requires inactivation of the early endosomal GTPase Rab5 (herein referring to generically to all isoforms). Here, we show that HD-PTP links ESCRT function with endosomal...

... (APP), but the intracellular site of Aβ production is unclear. APP has been localized to multivesicular bodies (MVBs) where sorting of APP onto intraluminal vesicles (ILVs) could promote amyloidogenic processing, or reduce Aβ production or accumulation by sorting APP and processing products...

.... To determine the specific site of prion conversion, we knocked down various proteins in the endocytic pathway including Rab7a, Tsg101 and Hrs (also known as HGS). PrPsc was markedly reduced in two chronically infected cell lines by preventing the maturation of the multivesicular body, a process that begins...

... protein Btn2. * Author for correspondence ( [email protected] ) Competing interests The authors declare no competing or financial interests. 17 07 2014 5 12 2014 © 2015. Published by The Company of Biologists Ltd 2015 Epsin Multivesicular body Ubiquitin ESCRT...

... that ligand uptake through LDLR family members controls oligodendrocyte differentiation. These findings identify NDRG1 as a novel regulator of multivesicular body formation and endosomal LDLR trafficking. The deficiency of functional NDRG1 in CMT4D might impair lipid processing and differentiation...

...   S. D. ( 2002 ). Receptor downregulation and multivesicular-body sorting.   Nat. Rev. Mol. Cell Biol.   3 , 893 – 905 . 10.1038/nrm973 Lapaque   N. , Jahnke   M. , Trowsdale   J. , Kelly   A. P. ( 2009 ). The HLA-DRalpha chain is modified by polyubiquitination.   J. Biol...

... MVBs and individual cisternae of the class E compartments are shown in various colors; vacuoles are shown as large red membranes. Scale bars: 100 nm. ( B ) Quantification of the frequency of three endosomal morphologies: multivesicular body (MVB), vesicular tubular endosome (VTE), and class E...

... -spectrin (β H ) and the multivesicular body (MVB) in Drosophila . AnxB9 binds to a subset of β H spliceoforms, and loss of AnxB9 results in an increase in basolateral β H and its appearance on cytoplasmic vesicles that overlap with the MVB markers Hrs, Vps16 and EPS15. Similar colocalizations are seen when...

... for their role in sorting ubiquitylated membrane proteins into multivesicular bodies. In this Opinion article, we discuss four possible models that could explain the observation that autophagosomes accumulate in ESCRT-depleted cells. We propose that the involvement of ESCRT proteins in the fusion...

..., and thus resemble multivesicular bodies. To determine whether multivesicular body endosomal intermediates are formed in Apicomplexa, we used the Plasmodium falciparum homolog of the class E gene, Vps4 , as a probe. Endogenous P. falciparum Vps4 (PfVps4) localized to the cytoplasm of P. falciparum...

...., Moehren, G., Ullrich, A., Schlessinger, J. and Hopkins, C. R. ( 1990 ). Kinase activity controls the sorting of the epidermal growth factor receptor within the multivesicular body. Cell 61 , 623 -634. Flynn, P., Mellor, H., Palmer, R., Panayotou, G. and Parker, P. J. ( 1998 ). Multiple...

... for mvb sorting. Dev. Cell 3 , 271 -282. Babst, M., Katzmann, D. J., Snyder, W. B., Wendland, B. and Emr, S. D. ( 2002b ). Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body. Dev. Cell 3 , 283 -289. Basyuk, E., Galli, T...

... into the lumen of the compartment; this process results in the formation of multivesicular bodies (MVBs). Fusion of MVBs with the plasma membrane results in secretion of the small internal vesicles, termed exosomes. K562 cells release exosomes with similar characteristics to reticulocyte exosomes, in particular...

... been shown to bind B-lymphocyte-derived exosomes at their cell surface, which supports the notion that exosomes play an immunoregulatory role. Finally, since exosomes are derived from multivesicular bodies, their molecular composition might provide clues to the mechanism of protein and lipid sorting...

... A. One of the newly isolated mutants, LEX2, fell into the complementation group B, and showed slower degradation of RET-LDL than LEX1 cells. LEX2 showed prominence of well-elaborated multivesicular bodies (MVBs), positive for lysosomal glycoprotein-B/cathepsin D and cation-independent mannose 6-phosphate...

... © 1997 by Company of Biologists 1997 N -ethylmaleimide Endocytic pathway Lysosome Endosomal carrier vesicle Multivesicular body Following endocytosis cell surface receptors and their ligands, as well as other membrane proteins, are sorted within the early endosome, located...

... that endocytosis may be acting to remove excess plasma membrane material added during exocytosis of secretory vesicles. 27 12 1989 13 06 1990 © 1990 by Company of Biologists 1990 endocytosis multivesicular body membrane recycling Endocytosis has been demonstrated in isolated...