...Masatoshi Takagi; Takao Ono; Toyoaki Natsume; Chiyomi Sakamoto; Mitsuyoshi Nakao; Noriko Saitoh; Masato T. Kanemaki; Tatsuya Hirano; Naoko Imamoto ABSTRACT Although condensins play essential roles in mitotic chromosome assembly, Ki-67 (also known as MKI67), a protein localizing to the periphery...

... for anti-HP1 antibody (a gift of L. Wallrath, University of Iowa, Iowa City, IA), and 1:200 for anti-MSL2 antibody (a gift of M. Kuroda, Harvard Medical School, Boston, MA) and for anti-H4AcK16 antibody (a gift of B. Turner, University of Birmingham, UK). Immunostaining of mitotic chromosomes...

..., were efficiently targeted to nucleoli, whereas a repetitive structure found at the C-terminal portion, whose similarity to pKi-67 is weak, was localized precisely to mitotic chromosomes. The C-terminal portion was designated the ‘LR domain’ since several LR (leucine and arginine) pairs commonly appear...