... microtubule–kinetochore attachments to induce spindle assembly checkpoint (SAC; also known as the mitotic checkpoint)-activated mitotic arrest. SAC activation causes mitotic arrest by chronically activating Cdk1. One consequence of prolonged Cdk1 activation is cell death. However, the cytoplasmic signal(s...

...Ikram Mossaid; Guillaume Chatel; Valérie Martinelli; Marcela Vaz; Birthe Fahrenkrog ABSTRACT Nucleoporin Nup153 is a multifunctional protein and a known binding partner of mitotic checkpoint protein Mad1 (also known as MAD1L1). The functional relevance of their interaction has remained elusive...

... Knl1 MELT motif Kinetochore Mitosis Mitotic checkpoint Knl1, also known as Spc105 in budding yeast, Spc7 in fission yeast, and in humans as CASC5, AF15q14 and Blinkin, is a central component of the Knl1–Mis12–Ndc80 (KMN) network, a ten-subunit macromolecular assembly that also comprises...

... in anaphase, that promotes the segregation of merotelically oriented chromosomes to the correct pole. * Author for correspondence (e-mail: [email protected] ) 19 6 2003 © The Company of Biologists Limited 2003 2003 Merotelic orientation Lagging chromosomes Mitotic checkpoint...

.... Kinetochores of lagging chromosomes in anaphase human cells were found to be devoid of the mitotic checkpoint phosphoepitopes recognized by the 3F3/2 antibody, suggesting that they attached kinetochore microtubules prior to anaphase onset. Live cell imaging of H2B histone-GFP-transfected cells showed...

... Centromere Centrosome Mitotic checkpoint Drosophila During early stages of mitosis in higher eukaryotic cells, the fully matured kinetochores capture spindle microtubules. A major consequence of this process is that pole-to-kinetochore microtubules become stabilised. This is a pre-requisite...