... with Tim18, whereas TM3 is exclusively required for the interaction with Sdh3. Moreover, impairment of TIM22 complex assembly influences its translocase activity, the mitochondrial network, and the viability of cells lacking mitochondrial DNA. Overall, our findings provide compelling evidence highlighting...

...Laurent Chatre; Miria Ricchetti Summary Mitochondrial DNA (mtDNA) replication and transcription are crucial for cell function, but these processes are poorly understood at the single-cell level. We describe a novel fluorescence in situ hybridization protocol, called mTRIP (mitochondrial...

... biogenesis Mitochondria Mitochondrial DNA For decades, electron microscopists have observed that organelles such as the endoplasmic reticulum (ER) and mitochondria are physically connected ( Robertson, 1960 ; Mannella et al., 1998 ), and electron-dense structures that bridge the two organelles...

...Martin Kucej; Blanka Kucejova; Ramiah Subramanian; Xin Jie Chen; Ronald A. Butow Mitochondrial DNA is organized as a nucleoprotein complex called the nucleoid. Its major protein components have been identified in different organisms, but it is yet unknown whether nucleoids undergo any form...

...., Nakayama, H., Takio, K., Hamasaki, N. and Kang, D. ( 2003 ). Human mitochondrial DNA is packaged with TFAM. Nucleic Acids Res. 31 , 1640 -1645. Anderson, S., Bankier, A. T., Barrell, B. G., de Bruijn, M. H., Coulson, A. R., Drouin, J., Eperon, I. C., Nierlich, D. P., Roe, B. A., Sanger, F. et al...

... by CO activates mitochondrial biogenesis in the heart. In mice, transient elevations of cellular CO by five- to 20-fold increased the copy number of cardiac mitochondrial DNA, the content of respiratory complex I-V and interfibrillar mitochondrial density within 24 hours. Mitochondrial biogenesis...

... (mitochondrial DNA absence sensitive factor) whose expression was enhanced by the absence of mitochondrial DNA (mtDNA). In patients with mitochondrial diseases, MIDAS expression was increased only in dysfunctional muscle fibers. A majority of MIDAS localized to mitochondria with a small fraction in the Golgi...

... we created ρ Δ cells with a reduced amount (about 1/4) of mitochondrial DNA (mtDNA) from Dictyostelium discoideum Ax-2 cells, by exposing Ax-2 cells to ca. 30 μg/ml of ethidium bromide (EtBr) in axenic growth medium. Importantly, the ρ Δ cells exhibited a series of fascinating behaviors: when...