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Keywords: Microtubule nucleation
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J Cell Sci (2023) 136 (12): jcs261096.
Published: 26 June 2023
... with importins. A major consequence of Ran-GTP regulation is the local stimulation of branched microtubule nucleation around chromosomes, which is mediated by the augmin complex (composed of the eight subunits HAUS1-HAUS8), a process that is crucially important for correct spindle assembly. However, augmin...
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Cytoskeleton
J Cell Sci (2021) 134 (14): jcs245464.
Published: 23 July 2021
... microtubules are made in the cell and provides a basis for an exciting era in the cytoskeleton field. Cytoskeleton Microtubule Microtubule nucleation Princeton University http://dx.doi.org/10.13039/100006734 National Institutes of Health http://dx.doi.org/10.13039/100000002...
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Cytoskeleton
J Cell Sci (2020) 133 (11): jcs244368.
Published: 3 June 2020
.... 21 1 2020 26 3 2020 © 2020. Published by The Company of Biologists Ltd 2020 http://www.biologists.com/user-licence-1-1/ Highlighted Article: γ-tubulin ring complexes are templates for microtubule nucleation and are composed of γ-tubulin and γ-tubulin complex proteins (GCPs...
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Imaging
J Cell Sci (2019) 132 (3): jcs225557.
Published: 1 February 2019
... of the Golgi depends on its association with a septin 1 (SEPT1)-based scaffold, which promotes local microtubule nucleation and positioning of the Golgi. SEPT1 function depends on the Golgi matrix protein GM130 (also known as GOLGA2) and on centrosomal proteins, including CEP170 and components of γ-tubulin...
Includes: Supplementary data
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J Cell Sci (2019) 132 (4): jcs219501.
Published: 9 November 2018
... can self-assemble into condensates that recruit α- and β-tubulins. In Xenopus extracts, PLK4 assemblies additionally recruit STIL, a substrate of PLK4, and the microtubule nucleator γ-tubulin, forming acentriolar MTOCs de novo . The assembly of these robust microtubule asters is independent of dynein...
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J Cell Sci (2012) 125 (19): 4445–4456.
Published: 1 October 2012
... understood. γ-tubulin has been found in complexes of various sizes but several lines of evidence suggest that only large, ring-shaped complexes function as efficient microtubule nucleators. Human γ-tubulin ring complexes (γTuRCs) are composed of γ-tubulin and the γ-tubulin complex components (GCPs) 2, 3, 4...
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J Cell Sci (2009) 122 (13): 2208–2217.
Published: 1 July 2009
... biosynthesis and cell elongation. Plant Cell 13 , 807 -827. Chan, J., Calder, G. M., Doonan, J. H. and Lloyd, C. W. ( 2003 ). EB1 reveals mobile microtubule nucleation sites in Arabidopsis. Nat. Cell Biol. 5 , 967 -971. Chrétien, D. and Wade, R. H. ( 1991 ). New data on the microtubule...
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J Cell Sci (2001) 114 (2): 413–422.
Published: 15 January 2001
...Yuki F. Inclán; Eva Nogales ABSTRACT αβ-tubulin heterodimers self-assemble to form microtubules nucleated by γ-tubulin in the cell. γ-tubulin is believed to recruit the αβ-tubulin dimers that form the minus ends of microtubules, but the molecular mechanism of this action remains a matter of heated...
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J Cell Sci (1997) 110 (20): 2533–2545.
Published: 15 October 1997
... is phosphorylated during mitosis and is tightly associated with the centrosome: only denaturing conditions such as 8 M urea were able to solubilise it. Purified immunoglobulins directed against Spc110p inhibit microtubule nucleation on isolated human centrosomes, using brain phosphocellulosetubulin or Xenopus egg...
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J Cell Sci (1997) 110 (3): 295–300.
Published: 1 February 1997
... different roles for γ-tubulin and the other proteins in the complex in tubulin assembly. While the structure and composition of a microtubule nucleation site is becoming clearer, it is still unknown how the cell-cycle dependent regulation of microtubule nucleation sites is achieved and whether...
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J Cell Sci (1996) 109 (11): 2755–2766.
Published: 1 November 1996
... EGS-seed Biotinylated Tubulin Coupled GTP hydrolysis GDP assay Microtubule nucleation Fig. 6. Variation of GDP-release rate with Tu-GTP concentration. (A) Rates of GDP regeneration vs tubulin concentration, for B-EGS-seeds added to sub-C C [Tu-GTP] ( Fig. 5 data): (a) least-squares fit...
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