... with importins. A major consequence of Ran-GTP regulation is the local stimulation of branched microtubule nucleation around chromosomes, which is mediated by the augmin complex (composed of the eight subunits HAUS1-HAUS8), a process that is crucially important for correct spindle assembly. However, augmin...

... , D. A. ( 2005 ). Insights into microtubule nucleation from the crystal structure of human gamma-tubulin . Nature 435 , 523 - 527 . 10.1038/nature03586 Alfaro-Aco , R. , Thawani , A. and Petry , S. ( 2017 ). Structural analysis of the role of TPX2 in branching...

.... 21 1 2020 26 3 2020 © 2020. Published by The Company of Biologists Ltd 2020 http://www.biologists.com/user-licence-1-1/ Highlighted Article: γ-tubulin ring complexes are templates for microtubule nucleation and are composed of γ-tubulin and γ-tubulin complex proteins (GCPs...

... of the Golgi depends on its association with a septin 1 (SEPT1)-based scaffold, which promotes local microtubule nucleation and positioning of the Golgi. SEPT1 function depends on the Golgi matrix protein GM130 (also known as GOLGA2) and on centrosomal proteins, including CEP170 and components of γ-tubulin...

... can self-assemble into condensates that recruit α- and β-tubulins. In Xenopus extracts, PLK4 assemblies additionally recruit STIL, a substrate of PLK4, and the microtubule nucleator γ-tubulin, forming acentriolar MTOCs de novo . The assembly of these robust microtubule asters is independent of dynein...

... understood. γ-tubulin has been found in complexes of various sizes but several lines of evidence suggest that only large, ring-shaped complexes function as efficient microtubule nucleators. Human γ-tubulin ring complexes (γTuRCs) are composed of γ-tubulin and the γ-tubulin complex components (GCPs) 2, 3, 4...

... biosynthesis and cell elongation. Plant Cell 13 , 807 -827. Chan, J., Calder, G. M., Doonan, J. H. and Lloyd, C. W. ( 2003 ). EB1 reveals mobile microtubule nucleation sites in Arabidopsis. Nat. Cell Biol. 5 , 967 -971. Chrétien, D. and Wade, R. H. ( 1991 ). New data on the microtubule...

... Drosophila Microtubule nucleation ZP domain Cellular adhesion to the extracellular matrix (ECM) is an essential part of the development of tissues and the continued maintenance of their integrity ( Gumbiner, 1996 ). The Drosophila wing has proved to be a useful model system for studying cellular...

...Yuki F. Inclán; Eva Nogales ABSTRACT αβ-tubulin heterodimers self-assemble to form microtubules nucleated by γ-tubulin in the cell. γ-tubulin is believed to recruit the αβ-tubulin dimers that form the minus ends of microtubules, but the molecular mechanism of this action remains a matter of heated...

... is phosphorylated during mitosis and is tightly associated with the centrosome: only denaturing conditions such as 8 M urea were able to solubilise it. Purified immunoglobulins directed against Spc110p inhibit microtubule nucleation on isolated human centrosomes, using brain phosphocellulosetubulin or Xenopus egg...

... different roles for γ-tubulin and the other proteins in the complex in tubulin assembly. While the structure and composition of a microtubule nucleation site is becoming clearer, it is still unknown how the cell-cycle dependent regulation of microtubule nucleation sites is achieved and whether...

... is small and independent of elongation rate . J. Cell Biol . 114 , 73 – 81 . 10.1083/jcb.114.1.73 EGS-seed Biotinylated Tubulin Coupled GTP hydrolysis GDP assay Microtubule nucleation Dynamic instability of a population of microtubules assembled from tubulin dimer (Tu) gives...

..., a component of both interphase and mitotic spindle pole body, in formation of the microtubule nucleating complex at the mitotic spindle pole body. A polyclonal antibody specific to S. pombe γ-tubulin inhibited both activation of the interphase spindle pole body and microtubule nucleation from the mitotic...