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1-20 of 168
Keywords: Microtubule
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Journal Articles
Mustafa G. Aydogan, Laura E. Hankins, Thomas L. Steinacker, Mohammad Mofatteh, Saroj Saurya, Alan Wainman, Siu-Shing Wong, Xin Lu, Felix Y. Zhou, Jordan W. Raff
Journal:
Journal of Cell Science
J Cell Sci jcs.260015.
Published: 16 June 2022
...Mustafa G. Aydogan; Laura E. Hankins; Thomas L. Steinacker; Mohammad Mofatteh; Saroj Saurya; Alan Wainman; Siu-Shing Wong; Xin Lu; Felix Y. Zhou; Jordan W. Raff Centrioles are composed of a central cartwheel tethered to nine-fold symmetric microtubule (MT) blades. The centriole cartwheel and MTs...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci jcs.259623.
Published: 15 June 2022
... in this mechanism of actin, Rho GTPases and microtubules, which are major actors of CIE processes. We show that the actin cytoskeleton is dynamically associated with endoA3- and CD166-positive endocytic carriers and that its perturbation strongly inhibits the uptake process of CD166. We also reveal that the Rho...
Journal Articles
In collection:
Cytoskeleton
Journal:
Journal of Cell Science
J Cell Sci (2023) 136 (5): jcs260144.
Published: 31 May 2022
...Toni McHugh; Julie P. I. Welburn ABSTRACT The precise regulation of microtubule length during mitosis is essential to assemble and position the mitotic spindle and segregate chromosomes. The kinesin-13 Kif2C or MCAK acts as a potent microtubule depolymerase that diffuses short distances...
Includes: Supplementary data
Journal Articles
In collection:
Cytoskeleton
Journal:
Journal of Cell Science
J Cell Sci (2022) 135 (9): jcs259539.
Published: 6 May 2022
...Emmanuel T. Nsamba; Mohan L. Gupta, Jr ABSTRACT The microtubule cytoskeleton is assembled from the α- and β-tubulin subunits of the canonical tubulin heterodimer, which polymerizes into microtubules, and a small number of other family members, such as γ-tubulin, with specialized functions. Overall...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2022) 135 (7): jcs259234.
Published: 8 April 2022
...Joseph Atherton; Melissa Stouffer; Fiona Francis; Carolyn A. Moores ABSTRACT Neurons extend axons to form the complex circuitry of the mature brain. This depends on the coordinated response and continuous remodelling of the microtubule and F-actin networks in the axonal growth cone. Growth cone...
Includes: Supplementary data
Journal Articles
In collection:
Cytoskeleton
Mira Kuzmić, Gerard Castro Linares, Jindřiška Leischner Fialová, François Iv, Danièle Salaün, Alex Llewellyn, Maxime Gomes, Mayssa Belhabib, Yuxiang Liu, Keisuke Asano, Magda Rodrigues, Daniel Isnardon, Taro Tachibana, Gijsje H. Koenderink, Ali Badache, Manos Mavrakis, Pascal Verdier-Pinard
Journal:
Journal of Cell Science
J Cell Sci (2022) 135 (1): jcs258850.
Published: 10 January 2022
... ABSTRACT Septins, a family of GTP-binding proteins that assemble into higher order structures, interface with the membrane, actin filaments and microtubules, and are thus important regulators of cytoarchitecture. Septin 9 (SEPT9), which is frequently overexpressed in tumors and mutated in hereditary...
Includes: Supplementary data
Journal Articles
In collection:
Cytoskeleton
Journal:
Journal of Cell Science
J Cell Sci (2021) 134 (14): jcs245464.
Published: 23 July 2021
...Akanksha Thawani; Sabine Petry ABSTRACT As one of four filament types, microtubules are a core component of the cytoskeleton and are essential for cell function. Yet how microtubules are nucleated from their building blocks, the αβ-tubulin heterodimer, has remained a fundamental open question since...
Journal Articles
In collection:
Cytoskeleton
Journal:
Journal of Cell Science
J Cell Sci (2021) 134 (14): jcs254037.
Published: 23 July 2021
...Janet K. Jang; Amy C. Gladstein; Arunika Das; Joanatta G. Shapiro; Zachary L. Sisco; Kim S. McKim ABSTRACT Meiosis in female oocytes lacks centrosomes, the microtubule-organizing centers. In Drosophila oocytes, meiotic spindle assembly depends on the chromosomal passenger complex (CPC...
Includes: Supplementary data
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2021) 134 (13): jcs258464.
Published: 8 July 2021
...T. Lynne Blasius; Yang Yue; RaghuRam Prasad; Xinglei Liu; Arne Gennerich; Kristen J. Verhey ABSTRACT The kinesin-4 member KIF7 plays critical roles in Hedgehog signaling in vertebrate cells. KIF7 is an atypical kinesin as it binds to microtubules but is immotile. We demonstrate that, like...
Includes: Supplementary data
Journal Articles
Chengye Feng, Joseph M. Cleary, Gregory O. Kothe, Michelle C. Stone, Alexis T. Weiner, James I. Hertzler, William O. Hancock, Melissa M. Rolls
Journal:
Journal of Cell Science
J Cell Sci (2021) 134 (11): jcs258437.
Published: 7 June 2021
...Chengye Feng; Joseph M. Cleary; Gregory O. Kothe; Michelle C. Stone; Alexis T. Weiner; James I. Hertzler; William O. Hancock; Melissa M. Rolls ABSTRACT Axons and dendrites are distinguished by microtubule polarity. In Drosophila , dendrites are dominated by minus-end-out microtubules, whereas axons...
Includes: Supplementary data
Journal Articles
In collection:
Cytoskeleton
Journal:
Journal of Cell Science
J Cell Sci (2021) 134 (10): jcs257543.
Published: 3 June 2021
...Neha Khetan; Gérard Pruliere; Celine Hebras; Janet Chenevert; Chaitanya A. Athale ABSTRACT Radial microtubule (MT) arrays or asters determine cell geometry in animal cells. Multiple asters interacting with motors, such as those in syncytia, form intracellular patterns, but the mechanical principles...
Includes: Supplementary data
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2021) 134 (10): jcs250001.
Published: 27 May 2021
...Patricia Wadsworth ABSTRACT During anaphase, a microtubule-containing structure called the midzone forms between the segregating chromosomes. The midzone is composed of an antiparallel array of microtubules and numerous microtubule-associated proteins that contribute to midzone formation...
Includes: Supplementary data
Journal Articles
In collection:
Cytoskeleton
Journal:
Journal of Cell Science
J Cell Sci (2021) 134 (4): jcs246496.
Published: 17 February 2021
... play an important role in cellular function and morphology. The biophysical properties of cells and tissues are determined by the cytoskeleton, consisting of dynamic networks of F-actin and microtubules, molecular motors, crosslinkers and other associated proteins, among other factors such as cell–cell...
Journal Articles
In collection:
Cilia and Flagella
Journal:
Journal of Cell Science
J Cell Sci (2020) 133 (17): jcs249805.
Published: 11 September 2020
.... Using Chlamydomonas , we attenuated IFT-based tubulin transport of GFP–β-tubulin by altering the IFT74N–IFT81N tubulin-binding module and the C-terminal E-hook of tubulin. E-hook-deficient GFP–β-tubulin was incorporated into the axonemal microtubules, but its transport frequency by IFT was reduced...
Includes: Supplementary data
Journal Articles
Christopher Nosala, Kari D. Hagen, Nicholas Hilton, Tiffany M. Chase, Kelci Jones, Rita Loudermilk, Kristofer Nguyen, Scott C. Dawson
Journal:
Journal of Cell Science
J Cell Sci (2020) 133 (16): jcs227355.
Published: 27 August 2020
...Christopher Nosala; Kari D. Hagen; Nicholas Hilton; Tiffany M. Chase; Kelci Jones; Rita Loudermilk; Kristofer Nguyen; Scott C. Dawson ABSTRACT Giardia lamblia , a widespread parasitic protozoan, attaches to the host gastrointestinal epithelium by using the ventral disc, a complex microtubule (MT...
Includes: Supplementary data
Journal Articles
In collection:
Exploring the Nucleus
Thibault Legal, Daniel Hayward, Agata Gluszek-Kustusz, Elizabeth A. Blackburn, Christos Spanos, Juri Rappsilber, Ulrike Gruneberg, Julie P. I. Welburn
Journal:
Journal of Cell Science
J Cell Sci (2020) 133 (16): jcs246025.
Published: 25 August 2020
... chromosome alignment during mitosis. CENP-E Motor Kinetochore Mitosis Microtubule To maintain their genomic integrity, eukaryotic cells must distribute their DNA equally to the daughter cells. Spindle microtubules mediate the segregation of chromosomes by associating with the kinetochore...
Includes: Supplementary data
Journal Articles
In collection:
Cilia and Flagella
Emiliya Poghosyan, Ioan Iacovache, Lenka Faltova, Alexander Leitner, Pinfen Yang, Dennis R. Diener, Ruedi Aebersold, Benoit Zuber, Takashi Ishikawa
Journal:
Journal of Cell Science
J Cell Sci (2020) 133 (16): jcs245233.
Published: 21 August 2020
... and biophysics data, this allowed localization of components in the radial spoke head and neck regions. Cilia Cryo-electron microscopy Dynein Flagella Microtubule Radial spoke Cross-link mass spectrometry Motile cilia and flagella are ubiquitous organelles present in many eukaryotic cells...
Includes: Supplementary data
Journal Articles
In collection:
Cell Biology and Disease
Anne Blangy, Guillaume Bompard, David Guerit, Pauline Marie, Justine Maurin, Anne Morel, Virginie Vives
Journal:
Journal of Cell Science
J Cell Sci (2020) 133 (13): jcs244798.
Published: 1 July 2020
... relies on the typical organization of osteoclast cytoskeleton components into a highly complex structure comprising actin, microtubules and other cytoskeletal proteins that constitutes the backbone of the bone resorption apparatus. The development of methods to differentiate osteoclasts in culture...
Journal Articles
In collection:
Cytoskeleton
Stephen Kershaw, David J. Morgan, James Boyd, David G. Spiller, Gareth Kitchen, Egor Zindy, Mudassar Iqbal, Magnus Rattray, Christopher M. Sanderson, Andrew Brass, Claus Jorgensen, Tracy Hussell, Laura C. Matthews, David W. Ray
Journal:
Journal of Cell Science
J Cell Sci (2020) 133 (11): jcs242842.
Published: 11 June 2020
..., in response to both GR agonist and antagonist ligand binding. The inhibitory effect on migration is prevented by GR knockdown with siRNA, confirming GR specificity, but not by actinomycin D treatment, suggesting a non-transcriptional mechanism. We identified a rapid onset increase in microtubule...
Includes: Supplementary data
Journal Articles
In collection:
Exploring the Nucleus
Journal:
Journal of Cell Science
J Cell Sci (2020) 133 (11): jcs240234.
Published: 8 June 2020
... between microtubule motors and non-motor proteins. Defects in spindle assembly or changes in spindle dynamics are associated with diseases, such as cancer or developmental disorders. Here, we compared mitotic and meiotic spindles in fission yeast. We show that, even though mitotic and meiotic spindles...
Includes: Supplementary data