Skip Nav Destination
1-20 of 38
J Cell Sci (2005) 118 (24): 5825–5834.
Published: 15 December 2005
.... Here, the nuclear distribution of microinjected fluorescein-labeled dextrans of 42 kDa to 2.5 MDa molecular mass was used to characterize the chromatin accessibility in dependence on histone acetylation. Measurements of the fluorescein-dextran sizes were combined with an image correlation spectroscopy...
J Cell Sci (2005) 118 (6): 1299–1307.
Published: 15 March 2005
... are assembled. Recent work in both yeast and mammalian cells has identified the nucleolus as a possible initial site of SRP assembly. In the present study, SRP RNA and protein components were identified in the extrachromosomal, amplified nucleoli of Xenopus laevis oocytes. Fluorescent SRP RNA microinjected...
Thomas Huff, Olaf Rosorius, Angela M. Otto, Christian S. G. Müller, Edda Ballweber, Ewald Hannappel, Hans Georg Mannherz
J Cell Sci (2004) 117 (22): 5333–5341.
Published: 15 October 2004
... transglutaminase; however, this labelling does not interfere with its interaction with G-actin. Here we show that after microinjection into intact cells, fluorescently labelled thymosin β 4 has a diffuse cytoplasmic and a pronounced nuclear staining. Enzymatic cleavage of fluorescently labelled thymosin β 4...
J Cell Sci (2004) 117 (14): 3061–3071.
Published: 15 June 2004
... microinjected into the nuclei of HeLa cells. Functional confirmation of both signals (1017-1028 and 1054-1066) was provided by transfection of human myeloma cells with plasmids containing the gene for a full-length human FLAG-topoisomerase fusion protein, mutated at hydrophobic amino acid residues in the export...
Terence Davis, Sim K. Singhrao, Fiona S. Wyllie, Michele F. Haughton, Paul J. Smith, Marie Wiltshire, David Wynford-Thomas, Christopher J. Jones, Richard G. A. Faragher, David Kipling
J Cell Sci (2003) 116 (7): 1349–1357.
Published: 1 April 2003
... cells re-entered the cell cycle following microinjection of a p53-neutralizing antibody. Similarly, production of the human papilloma virus 16 E6 oncoprotein in presenescent AG05229 cells resulted in senescence being bypassed and extended cellular life span. Werner-syndrome fibroblasts expressing E6 did...
Shin-ichiro Yoshimura, Nobuhiro Nakamura, Francis A. Barr, Yoshio Misumi, Yukio Ikehara, Hiroshi Ohno, Masao Sakaguchi, Katsuyoshi Mihara
J Cell Sci (2001) 114 (22): 4105–4115.
Published: 15 November 2001
... protein was still found in the ER membrane fractions. Second, epitope tagged GM130 and GRASP65 were expressed in NRK cells by plasmid microinjection into the nuclei and their localization was analyzed by immunofluorescence. When ER to Golgi transport was inhibited by prior microinjection of a GTP...
J Cell Sci (2000) 113 (7): 1167–1176.
Published: 1 April 2000
... integrin-specific due to a transdominant regulation of the α6β1 integrin by the α3β1 integrin. To determine the mechanism of this regulation, peptides representing the cytoplasmic domain of the α3 or α6 integrin subunits were microinjected together with recombinant enhanced green fluorescence protein...
J Cell Sci (1998) 111 (13): 1877–1888.
Published: 1 July 1998
...Rainer Pepperkok; Martin Lowe; Brian Burke; Thomas E. Kreis ABSTRACT Microinjected GTPγS revealed three distinct steps in the exocytic transport of the temperature sensitive glycoprotein of vesicular stomatitis virus (ts-O45-G) from the ER to the cell surface in intact Vero cells. While COPII...
J Cell Sci (1998) 111 (12): 1695–1706.
Published: 15 June 1998
... and NIH3T3 cultured cells by microinjection or by expression in conjunction with immunostaining and fluorescence microscopy. Microinjection of the actinfragmin dimer disintegrated the actin cytoskeleton and altered cell morphology. This in vivo effect could be blocked by phosphorylation of the actin subunit...
J Cell Sci (1998) 111 (3): 359–372.
Published: 1 February 1998
... enabled the visualization of the PNBs forming the dense fibrillar component (DFC) of new nucleoli. Anti-fibrillarin antibodies microinjected at different periods of mitosis blocked fibrillarin translocation at different steps, i.e. the formation of large foci, foci gathering in PNBs or PNB targeting...
J Cell Sci (1997) 110 (8): 1023–1032.
Published: 15 April 1997
...Christian Böker; Kurt von Figura; Annette Hille-Rehfeld ABSTRACT Recycling of mannose 6-phosphate receptors was investigated by microinjection of F ab fragments against their carboxy-terminal peptides (residues 54-67 or 150-164 of the cytoplasmic domain of 46 kDa and 300 kDa mannose 6-phosphate...
J Cell Sci (1997) 110 (6): 789–800.
Published: 15 March 1997
... domains, responsible for positioning tau on the microtubule surface, and the repeats which act as catalytic domains for microtubule assembly. To examine whether this model is appropriate in vivo we generated recombinant tau isoforms and microinjected them into CHO cells. Immunofluorescence microscopy...
J Cell Sci (1996) 109 (9): 2401–2406.
Published: 1 September 1996
... in an in vitro nuclear transport assay is not affected by these drugs. This observation has been confirmed for intact cells by microinjection experiments: cells which have been incubated with cytochalasin B or colchicine prior to microinjection of the reporter protein show the same accumulation in the nucleus...
F. Le Cahérec, P. Bron, J. M. Verbavatz, A. Garret, G. Morel, A. Cavalier, G. Bonnec, D. Thomas, J. Gouranton, J. F. Hubert
J Cell Sci (1996) 109 (6): 1285–1295.
Published: 1 June 1996
...F. Le Cahérec; P. Bron; J. M. Verbavatz; A. Garret; G. Morel; A. Cavalier; G. Bonnec; D. Thomas; J. Gouranton; J. F. Hubert ABSTRACT Xenopus laevis oocytes are widely used as an expression system for plasma membrane proteins, achieved by cyto-plasmic microinjection of messenger RNA. In the present...
J Cell Sci (1995) 108 (2): 581–593.
Published: 1 February 1995
... binding protein actin myofibrillogenesis muscle cell phosphatidylinositol 4,5-bisphosphate (PIP 2 ) microinjection Multiple actin-regulatory proteins have been characterized as being profoundly involved in various cell functions by regulating the assembly and disassembly of actin (for review...
J Cell Sci (1994) 107 (7): 1929–1934.
Published: 1 July 1994
... in the desmid Euastrum . J. Phycol . 29 , 667 – 674 . Zhang , D. , Wadsworth , P. and Hepler , P. K. ( 1990 ). Microtubule dynamics in living dividing plant cells. Confocal imaging of microinjected fluorescent brain tubulin . Proc. Nat. Acad. Sci. USA 87 , 8820 – 8824...
J Cell Sci (1993) 106 (4): 1179–1188.
Published: 1 December 1993
...Vladimir I. Rodionov; Vladimir I. Gelfand; Gary G. Borisy ABSTRACT To study the possible involvement of kinesin-like mole-cules in mitosis a polyclonal antibody against the head domain of Drosophila kinesin heavy chain (HD anti-body) was microinjected into PtK 1 cells at the prophase-prometaphase...
J Cell Sci (1993) 106 (2): 693–702.
Published: 1 October 1993
... the degradation rate of an injected protein? In Cell Fusion (ed. E. Evered and J. Whelan ), pp. 181 – 201 . London : Pitman Press . Sodeik , B. , Brix , K. and Stockem , W. ( 1989 ). Sequestration of microinjected molecular probes from the cytoplasm of Amoeba proteus...
J Cell Sci (1993) 104 (4): 1175–1185.
Published: 1 April 1993
... of topoisomerase II in living Drosophila blastoderm embryos. We show that the enzymatic activity can be specifically disrupted by microinjection of antitopoisomerase II antibodies as well as the epipodophyllotoxin VM26, a known inhibitor of topoisomerase II in vitro. By labeling the chromatin of live embryos...
J Cell Sci (1993) 104 (1): 139–150.
Published: 1 January 1993
...Markku Kallajoki; Jens Harborth; Klaus Weber; Mary Osborn ABSTRACT Several high molecular mass proteins which relocate from the interphase nucleus to the spindle poles during mitosis have been defined by antibodies. Microinjection experiments have shown that at least the antigen defined by SPN...