.... Here, the nuclear distribution of microinjected fluorescein-labeled dextrans of 42 kDa to 2.5 MDa molecular mass was used to characterize the chromatin accessibility in dependence on histone acetylation. Measurements of the fluorescein-dextran sizes were combined with an image correlation spectroscopy...

... are assembled. Recent work in both yeast and mammalian cells has identified the nucleolus as a possible initial site of SRP assembly. In the present study, SRP RNA and protein components were identified in the extrachromosomal, amplified nucleoli of Xenopus laevis oocytes. Fluorescent SRP RNA microinjected...

... transglutaminase; however, this labelling does not interfere with its interaction with G-actin. Here we show that after microinjection into intact cells, fluorescently labelled thymosin β 4 has a diffuse cytoplasmic and a pronounced nuclear staining. Enzymatic cleavage of fluorescently labelled thymosin β 4...

... microinjected into the nuclei of HeLa cells. Functional confirmation of both signals (1017-1028 and 1054-1066) was provided by transfection of human myeloma cells with plasmids containing the gene for a full-length human FLAG-topoisomerase fusion protein, mutated at hydrophobic amino acid residues in the export...

... cells re-entered the cell cycle following microinjection of a p53-neutralizing antibody. Similarly, production of the human papilloma virus 16 E6 oncoprotein in presenescent AG05229 cells resulted in senescence being bypassed and extended cellular life span. Werner-syndrome fibroblasts expressing E6 did...

... protein was still found in the ER membrane fractions. Second, epitope tagged GM130 and GRASP65 were expressed in NRK cells by plasmid microinjection into the nuclei and their localization was analyzed by immunofluorescence. When ER to Golgi transport was inhibited by prior microinjection of a GTP...

... integrin-specific due to a transdominant regulation of the α6β1 integrin by the α3β1 integrin. To determine the mechanism of this regulation, peptides representing the cytoplasmic domain of the α3 or α6 integrin subunits were microinjected together with recombinant enhanced green fluorescence protein...

...Rainer Pepperkok; Martin Lowe; Brian Burke; Thomas E. Kreis ABSTRACT Microinjected GTPγS revealed three distinct steps in the exocytic transport of the temperature sensitive glycoprotein of vesicular stomatitis virus (ts-O45-G) from the ER to the cell surface in intact Vero cells. While COPII...

... and NIH3T3 cultured cells by microinjection or by expression in conjunction with immunostaining and fluorescence microscopy. Microinjection of the actinfragmin dimer disintegrated the actin cytoskeleton and altered cell morphology. This in vivo effect could be blocked by phosphorylation of the actin subunit...

... enabled the visualization of the PNBs forming the dense fibrillar component (DFC) of new nucleoli. Anti-fibrillarin antibodies microinjected at different periods of mitosis blocked fibrillarin translocation at different steps, i.e. the formation of large foci, foci gathering in PNBs or PNB targeting...

...Christian Böker; Kurt von Figura; Annette Hille-Rehfeld ABSTRACT Recycling of mannose 6-phosphate receptors was investigated by microinjection of F ab fragments against their carboxy-terminal peptides (residues 54-67 or 150-164 of the cytoplasmic domain of 46 kDa and 300 kDa mannose 6-phosphate...

... domains, responsible for positioning tau on the microtubule surface, and the repeats which act as catalytic domains for microtubule assembly. To examine whether this model is appropriate in vivo we generated recombinant tau isoforms and microinjected them into CHO cells. Immunofluorescence microscopy...

... in an in vitro nuclear transport assay is not affected by these drugs. This observation has been confirmed for intact cells by microinjection experiments: cells which have been incubated with cytochalasin B or colchicine prior to microinjection of the reporter protein show the same accumulation in the nucleus...

...F. Le Cahérec; P. Bron; J. M. Verbavatz; A. Garret; G. Morel; A. Cavalier; G. Bonnec; D. Thomas; J. Gouranton; J. F. Hubert ABSTRACT Xenopus laevis oocytes are widely used as an expression system for plasma membrane proteins, achieved by cyto-plasmic microinjection of messenger RNA. In the present...

... compartment when radical and rapid changes are needed to modulate the digestive and absorptive processes. * Author for correspondence 08 09 1994 04 11 1994 © 1995 by Company of Biologists 1995 acidosome cytochalasin B membrane replacement membrane tubulation microinjection...

... binding protein actin myofibrillogenesis muscle cell phosphatidylinositol 4,5-bisphosphate (PIP 2 ) microinjection Multiple actin-regulatory proteins have been characterized as being profoundly involved in various cell functions by regulating the assembly and disassembly of actin (for review...

... the chloroplast. * Author for correspondence 30 10 1993 14 03 1994 © 1994 by Company of Biologists 1994 actin microfilament confocal laser scanning microscopy Micrasterias microinjection nuclear migration phalloidin Actin microfilaments (MFs) have been associated...

...Vladimir I. Rodionov; Vladimir I. Gelfand; Gary G. Borisy ABSTRACT To study the possible involvement of kinesin-like mole-cules in mitosis a polyclonal antibody against the head domain of Drosophila kinesin heavy chain (HD anti-body) was microinjected into PtK 1 cells at the prophase-prometaphase...

... 1993 © 1993 by Company of Biologists 1993 autophagic vacuole contractile vacuoles decorated spongiome Dictyostelium fluid segregation microinjection monoclonal antibody osmoregulation Paramecium Contractile vacuole complexes (CVCs), which are widely distributed...

... of topoisomerase II in living Drosophila blastoderm embryos. We show that the enzymatic activity can be specifically disrupted by microinjection of antitopoisomerase II antibodies as well as the epipodophyllotoxin VM26, a known inhibitor of topoisomerase II in vitro. By labeling the chromatin of live embryos...