... the two NSD3 isoforms present in somatic cells, the long isoform is responsible for regulating kollerin and cohesin chromatin-loading, and its methyltransferase activity is required for efficient sister chromatid cohesion. Based on these observations, we propose that NSD3-dependent methylation contributes...

... reproducible data and to minimize the clustering of fibers. We also demonstrate that the use of super-resolution microscopy is important for reliable evaluation of the distribution of histone modifications on individual fibers. Furthermore, we introduce a custom script for the analysis of methylation levels...

... 3 activation assays, chromatin immunoprecipitation and bisulfite sequencing PCR assays for methylation. Our results have implications for the development of strategies in CTA-based immune therapeutics. * These authors contributed equally to this work ‡ Authors for correspondence ( jlsang...

... for all methylation of H3 lysine 79 (H3K79), African trypanosomes express two DOT1 proteins that methylate histone H3K76 (corresponding to H3K79 in other organisms) in a cell-cycle-regulated manner. Whereas DOT1A is essential for normal cell cycle progression, DOT1B is involved in differentiation...

... an overview of these chromatin-related mechanisms and speculate on the extent to which they are conserved among eukaryotes. * Author for correspondence ( r.ketting@hubrecht.eu ) © 2010. 2010 Argonaute Chromatin Germ cell Methylation piRNA siRNA Although RNA was initially considered...

...-ADAMTS-12 antibody. We then washed the slides and stained nuclei with 4′-6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) at 1 μg/ml in Vectashield mounting medium (Vector Laboratories). Images were obtained using a confocal microscope, Leica TCS-SP2-AOBS. Methylation analysis of ADAMTS12...

...Ryan Heit; Jerome B. Rattner; Gordon K. T. Chan; Michael J. Hendzel Trimethylation of lysine 9 on histone H3 (H3K9me3) is known both to be necessary for proper chromosome segregation and to increase in late G2. We investigated the role of late G2 methylation, specifically in mitotic progression...

...Asmita Kumar; Sachin Kamboj; Barbara M. Malone; Shinichi Kudo; Jeffery L. Twiss; Kirk J. Czymmek; Janine M. LaSalle; N. Carolyn Schanen The methyl-CpG-binding protein 2 (MECP2) serves both organizational and transcriptional functions in the nucleus, with two well-characterized domains integrally...

...Natalia Dolzhanskaya; George Merz; John M. Aletta; Robert B. Denman FMRP, the fragile X mental retardation protein, is an RNA-binding protein that interacts with ∼4% of fetal brain mRNA. We have recently shown that a methyltransferase (MT) co-translationally methylates FMRP in vitro...

...Yinghong Ma; Stephanie B. Jacobs; Laurie Jackson-Grusby; Mary-Ann Mastrangelo; José A. Torres-Betancourt; Rudolf Jaenisch; Theodore P. Rasmussen In mammalian heterochromatin, cytosine bases of CpG dinucleotides are symmetrically modified by methylation. Patterns of CpG methylation are maintained...

... hybridisation and immunocytochemistry approach, we have analysed the molecular composition of this highly organised neuronal chromatin, to investigate its functional significance. We find that clusters of inactive, methylated rDNA repeats are present inside large neuronal nucleoli, which are often attached...

... showing that expression of R-genome rRNA genes, normally suppressed, is activated by 5-AC treatment and remains partially activated in the next generation. The distribution of 5-methylcytosine (5-mC) was studied using an antibody to 5-mC. Methylation was detected along the lengths of all chromosomes...

.... , Boyes , J. and Bird , A. ( 1990 ). High levels of de novo methylation and altered chromatin structure at CpG islands in cell lines . Cell 62 , 503 – 514 . 10.1016/0092-8674(90)90015-7 Basu , A. K. , Hanrahan , C. J. , Malia , S. A. , Kumar , S. , Bizanek , R...