... that the tension exerted by the ECM (through integrin-containing adhesion) generates defined actin-cytoskeleton force fields within cells during interphase (Thery et al., 2005). During metaphase, cells round up to undergo mitosis, but retraction fibres transfer the polarity of tension that was established...

...Jun-Yong Huang; Gary Morley; Deyu Li; Michael Whitaker Anaphase-promoting complex or cyclosome (APC/C) controls the metaphase-to-anaphase transition and mitosis exit by triggering the degradation of key cell cycle regulators such as securin and B-type cyclins. However, little is known about...

...Heng-Yu Chang; Mark Levasseur; Keith T. Jones Metaphase II-arrested mouse eggs are stimulated to complete meiosis by sperm-induced Ca 2+ spiking. The Ca 2+ signal causes activation of the E3 ligase anaphase-promoting complex/cyclosome (APC), leading to the destruction of key proteins necessary...

...Sarah E. Lee; Sanne Jensen; Lisa M. Frenz; Anthony L. Johnson; Didier Fesquet; Leland H. Johnston In eukaryotes an abnormal spindle activates a conserved checkpoint consisting of the MAD and BUB genes that results in mitotic arrest at metaphase. Recently, we and others identified a novel Bub2...

...R. V. Silverman-Gavrila; A. Forer ABSTRACT We studied the effects of various drugs on the poleward flux of tubulin in kinetochore microtubules in metaphase-I crane-fly spermatocytes. We used as a measure of tubulin flux a ‘gap’ in acetylation of kinetochore microtubules immediately poleward from...

... cycle, and becomes slightly heavier in mitosis than interphase. Cut9 in the 20S complex is hyperphosphorylated specifically at the time of metaphase. The truncated forms of Cut9 block entry into mitosis, however. The 20S assembly impaired in the cut9 mutant can be restored by elevating the level...

... for correspondence © 1993 by Company of Biologists 1993 mitosis mutant metaphase anaphase aar Drosophila The value of applying genetics to the study of complex cellular processes is derived from its ability to identify important, but relatively low-abundance components, and to provide...

...Victoria E. Centonze; Gary G. Borisy ABSTRACT Metaphase spindles can be induced to shrink by treating cells with microtubule-depolymerizing agents. During treatment, the paired sister chromatids remain at the metaphase plate and the poles move toward them. The question we asked is whether this pole...

... is currently controversial. We have examined the stability of kinetochore microtubules in metaphase PtKj spindles at 23 °C when microtubule assembly is abruptly blocked with the drug nocodazole. Metaphase cells were incubated in medium containing 34 pM nocodazole for various times before fixation...

...Christine J. Harrison; Elspeth M. Jack; Terence D. Allen; Rodney Harris ABSTRACT A technique has been developed to examine the same G-banded human metaphase chromosomes, first in the light microscope and then in the scanning electron microscope (SEM). A structural involvement in chromosome banding...

... phosphorylation. Owing to a stimulation of glycolysis, the latter agent does not decrease intracellular ATP in HeLa but does cause mitochondria to release calcium ions. Our data show that, at low concentrations, both agents prolong metaphase but differ in their effects on anaphase and cytokinesis. Studies...

...Peter Jeppesen; Helen Morten ABSTRACT Dehistonized metaphase chromosomes lose their apparent axial organization (the ‘scaffold’) and sediment more slowly following exposure to β-mercaptoethanol (BME). We have subsequently treated BME chromosomes with reagents that oxidize protein sulphydryls...