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Keywords: Metaphase
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Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2009) 122 (2): 171–177.
Published: 15 January 2009
... that the tension exerted by the ECM (through integrin-containing adhesion) generates defined actin-cytoskeleton force fields within cells during interphase (Thery et al., 2005). During metaphase, cells round up to undergo mitosis, but retraction fibres transfer the polarity of tension that was established...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2007) 120 (12): 1990–1997.
Published: 15 June 2007
...Jun-Yong Huang; Gary Morley; Deyu Li; Michael Whitaker Anaphase-promoting complex or cyclosome (APC/C) controls the metaphase-to-anaphase transition and mitosis exit by triggering the degradation of key cell cycle regulators such as securin and B-type cyclins. However, little is known about...
Journal Articles
Degradation of APC cdc20 and APC cdh1 substrates during the second meiotic division in mouse eggs
Available to PurchaseIn collection:
Proteostasis
Journal:
Journal of Cell Science
J Cell Sci (2004) 117 (26): 6289–6296.
Published: 15 December 2004
...Heng-Yu Chang; Mark Levasseur; Keith T. Jones Metaphase II-arrested mouse eggs are stimulated to complete meiosis by sperm-induced Ca 2+ spiking. The Ca 2+ signal causes activation of the E3 ligase anaphase-promoting complex/cyclosome (APC), leading to the destruction of key proteins necessary...
Journal Articles
The Bub2-dependent mitotic pathway in yeast acts every cell cycle and regulates cytokinesis
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Journal:
Journal of Cell Science
J Cell Sci (2001) 114 (12): 2345–2354.
Published: 15 June 2001
...Sarah E. Lee; Sanne Jensen; Lisa M. Frenz; Anthony L. Johnson; Didier Fesquet; Leland H. Johnston In eukaryotes an abnormal spindle activates a conserved checkpoint consisting of the MAD and BUB genes that results in mitotic arrest at metaphase. Recently, we and others identified a novel Bub2...
Journal Articles
Evidence that actin and myosin are involved in the poleward flux of tubulin in metaphase kinetochore microtubules of crane-fly spermatocytes
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Journal:
Journal of Cell Science
J Cell Sci (2000) 113 (4): 597–609.
Published: 15 February 2000
...R. V. Silverman-Gavrila; A. Forer ABSTRACT We studied the effects of various drugs on the poleward flux of tubulin in kinetochore microtubules in metaphase-I crane-fly spermatocytes. We used as a measure of tubulin flux a ‘gap’ in acetylation of kinetochore microtubules immediately poleward from...
Journal Articles
Distinct subunit functions and cell cycle regulated phosphorylation of 20S APC/cyclosome required for anaphase in fission yeast
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Journal:
Journal of Cell Science
J Cell Sci (1997) 110 (15): 1793–1804.
Published: 1 August 1997
... cycle, and becomes slightly heavier in mitosis than interphase. Cut9 in the 20S complex is hyperphosphorylated specifically at the time of metaphase. The truncated forms of Cut9 block entry into mitosis, however. The 20S assembly impaired in the cut9 mutant can be restored by elevating the level...
Journal Articles
Abnormal anaphase resolution (aar): a locus required for progression through mitosis in Drosophila
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Journal:
Journal of Cell Science
J Cell Sci (1993) 104 (2): 583–593.
Published: 1 February 1993
... for correspondence © 1993 by Company of Biologists 1993 mitosis mutant metaphase anaphase aar Drosophila The value of applying genetics to the study of complex cellular processes is derived from its ability to identify important, but relatively low-abundance components, and to provide...
Journal Articles
Pole-to-chromosome movements induced at metaphase: Sites of microtubule disassembly
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Journal:
Journal of Cell Science
J Cell Sci (1991) 100 (1): 205–211.
Published: 1 September 1991
...Victoria E. Centonze; Gary G. Borisy ABSTRACT Metaphase spindles can be induced to shrink by treating cells with microtubule-depolymerizing agents. During treatment, the paired sister chromatids remain at the metaphase plate and the poles move toward them. The question we asked is whether this pole...
Journal Articles
Stability of microtubule attachment to metaphase kinetochores in PTK 1 cells
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Journal:
Journal of Cell Science
J Cell Sci (1990) 96 (1): 9–15.
Published: 1 May 1990
... is currently controversial. We have examined the stability of kinetochore microtubules in metaphase PtKj spindles at 23 °C when microtubule assembly is abruptly blocked with the drug nocodazole. Metaphase cells were incubated in medium containing 34 pM nocodazole for various times before fixation...
Journal Articles
Light and scanning electron microscopy of the same human metaphase chromosomes
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Journal:
Journal of Cell Science
J Cell Sci (1985) 77 (1): 143–153.
Published: 1 August 1985
...Christine J. Harrison; Elspeth M. Jack; Terence D. Allen; Rodney Harris ABSTRACT A technique has been developed to examine the same G-banded human metaphase chromosomes, first in the light microscope and then in the scanning electron microscope (SEM). A structural involvement in chromosome banding...
Journal Articles
The alteration of mitotic events by ionophore a23187 and carbonyl cyanide N -chlorophenylhydrazone
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Journal:
Journal of Cell Science
J Cell Sci (1985) 75 (1): 347–355.
Published: 1 April 1985
... phosphorylation. Owing to a stimulation of glycolysis, the latter agent does not decrease intracellular ATP in HeLa but does cause mitochondria to release calcium ions. Our data show that, at low concentrations, both agents prolong metaphase but differ in their effects on anaphase and cytokinesis. Studies...
Journal Articles
Effects of sulphydryl reagents on the structure of dehistonized metaphase chromosomes
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Journal:
Journal of Cell Science
J Cell Sci (1985) 73 (1): 245–260.
Published: 1 February 1985
...Peter Jeppesen; Helen Morten ABSTRACT Dehistonized metaphase chromosomes lose their apparent axial organization (the ‘scaffold’) and sediment more slowly following exposure to β-mercaptoethanol (BME). We have subsequently treated BME chromosomes with reagents that oxidize protein sulphydryls...