...Christian Hoffmann; Dragomir Milovanovic ABSTRACT Liquid–liquid phase separation is a major mechanism for organizing macromolecules, particularly proteins with intrinsically disordered regions, in compartments not limited by a membrane or a scaffold. The cell can therefore be perceived as a complex...

...Alaumy Joshi; Travis H. Richard; Vishal M. Gohil ABSTRACT Studies of rare human genetic disorders of mitochondrial phospholipid metabolism have highlighted the crucial role that membrane phospholipids play in mitochondrial bioenergetics and human health. The phospholipid composition...

...Elias T. Spiliotis; W. James Nelson Septins are conserved GTP-binding proteins that associate with cellular membranes and the actin and microtubule cytoskeletons. They polymerize to form filamentous structures that act as diffusion barriers between different membrane domains and as molecular...

... ) 19 7 2005 © The Company of Biologists Limited 2005 2005 Resealing Tethering Fusion Membranes Calcium Cell survival in vivo often depends on the capacity for rapid repair or resealing of a plasma membrane disruption. Skeletal ( McNeil and Khakee, 1992 ) and cardiac muscle...

... nuclear envelope (membranes, pore complexes and underlying lamin filament network) from nucleoplasm and the majority of chromatin. Swelling proves reversible with addition of high molecular mass dextrans. Micropipette aspiration of swollen and unswollen nuclear envelopes is also reversible and yields...

...Sean P. Flaherty; Nicholas J. Swann ABSTRACT The guinea pig sperm acrosome reaction is characterized by a complex temporal and structural pattern of membrane fusions. In this study, we have used specific protease inhibitors to determine if proteases regulate this pattern of membrane fusions during...

...Christel R. Schopfer; Peter K. Hepler ABSTRACT The cellular pattern and distribution of membranes have been analyzed during cytokinesis in pollen mother cells of Tradescantia and compared with those of actin microfilaments (MFs) and microtubules (MTs). Membranes have been stained with DiOC 6 (3...

...R. E. Stephens ABSTRACT When the membranes of isolated embryonic cilia from three species of sea urchin are detergent-solubilized, the major proteins found are two equimolar polypeptides comigrating with tubulin subunits. Cross-reactivity with a variety of tubulin antibodies confirms their identity...

... domains suggests that MIP may be involved in the formation of close membrane appositions. Furthermore, previous ambiguities in the literature over the presence of MIP in gap junctions are clarified by the knowledge that, in situ , MIP associates strongly with gap junctions for only a brief period...

... the temperature-shift-induced change in SAg to follow the newly expressed antigen and the disappearing one, by both immunofluorescence and immunogold electron microscopy. The new SAg initially appeared scattered at the cell surface, over the ciliary and interciliary membrane domains, without any readily...

... membranes; followed by (3) formation of small cytoplasmic bridges (which allow for the exchange of molecular components only); before (4) their enlargement enables the cells to exchange micronuclei. This work concentrates on stages (2) and (3) in P. tetraurelia cells. We analysed lectin binding during...

...H. Plattner; R. Pape; B. Haacke; K. Olbricht; C. Westphal; H. Kersken ABSTRACT After the synchronous induction of exocytosis of secretory organelles (trichocysts) in Paramecium tetraurelia cells the process of membrane resealing and retrieval could be followed under synchronous conditions...