... to compress cells in vitro . Mechanobiology Mechanotransduction Membrane Cytoskeleton In vitro systems Mechanosensation Juan de La Cierva JDC2023-051559-I Spanish Ministry of Science and Innovation http://dx.doi.org/10.13039/501100004837 PID2022-142672NB-I00...

...Lalitha Sastry; Johnathan Rylee; Simpla Mahato; Andrew C. Zelhof ABSTRACT Specialized membrane and cortical protein regions are common features of cells and are utilized to isolate differential cellular functions. In Drosophila photoreceptors, the apical membrane domain is defined by two distinct...

... for AP-3 vesicle budding from the Golgi. Without these hinges, AP-3 remains associated with Golgi compartments as they mature. Adaptor Vesicle Golgi Membrane Transport National Institute of General Medical Sciences http://dx.doi.org/10.13039/100000057 R01 GM130644 R35 GM149202...

... as other cytoskeletal proteins. In vivo , septins interact with other cytoskeletal proteins and with the inner plasma membrane. Hence, bottom-up in vitro cell-free assays are well suited to dissect the roles and behavior of septins in a controlled environment. Specifically, in vitro studies have been...

...Koyomi Nakazawa; Gaurav Kumar; Brieuc Chauvin; Aurélie Di Cicco; Luca Pellegrino; Michael Trichet; Bassam Hajj; João Cabral; Anirban Sain; Stéphanie Mangenot; Aurélie Bertin ABSTRACT Septins are cytoskeletal proteins interacting with the inner plasma membrane and other cytoskeletal partners. Being...

...Karolina Sapoń; Rafał Mańka; Teresa Janas; Tadeusz Janas ABSTRACT The formation of membrane vesicles is a common feature in all eukaryotes. Lipid rafts are the best-studied example of membrane domains for both eukaryotes and prokaryotes, and their existence also is suggested in Archaea membranes...

... and promotes integrin–talin–actomyosin coupling. Actin Integrin Kindlin Membrane Talin Agence Nationale de la Recherche http://dx.doi.org/10.13039/501100001665 ANR-18-CE13-0026-01 ANR-21-CE13-0010-03 ANR-20-CE13-0016 Cell adhesion and migration are controlled by biochemical...

... with increased cell membrane damage, less efficient membrane repair and increased stiffness. Additionally, FSS treatment prevented the LNCaP FSS-sensitive cell line from forming a growing tumor in vivo . This suggests that these properties play a role in FSS resistance and could represent potential targets...

...Dalton Buysse; Anna-Katharina Pfitzner; Matt West; Aurélien Roux; Greg Odorizzi ABSTRACT The ESCRT-III protein complex executes reverse-topology membrane scission. The scission mechanism is unclear but is linked to remodeling of ESCRT-III complexes at the membrane surface. At endosomes, ESCRT-III...

...Satish Babu Moparthi; Thomas Wollert ABSTRACT Autophagy is one of the most elaborative membrane remodeling systems in eukaryotic cells. Its major function is to recycle cytoplasmic material by delivering it to lysosomes for degradation. To achieve this, a membrane cisterna is formed that gradually...

...Qian Feng; Benoît Kornmann ABSTRACT The intracellular environment of eukaryotic cells is highly complex and compact. The limited volume of the cell, usually a few hundred femtoliters, is not only occupied by numerous complicated, diverse membranous and proteinaceous structures, these structures...

... unilamellar vesicles and infectious X-31 influenza virus, and testing for the newly identified pore intermediate of membrane fusion, we observed ∼30–87% poration, depending upon lipid composition. Testing the hypothesis that spontaneous curvature (SC) of the lipid monolayer controls membrane poration, our...

...Peter G. Billcliff; Ruth Rollason; Ian Prior; Dylan M. Owen; Katharina Gaus; George Banting Summary The integral membrane protein tetherin has been associated with an eclectic mix of cellular processes, including restricting the release of a range of enveloped viruses from infected cells...

... and animal formins. We have identified for the first time a plant-specific region of the membrane-integrated formin AtFH4 that mediates an association with the microtubule cytoskeleton. In vitro analysis shows that this region (named the GOE domain) binds directly to microtubules. Overexpressed AtFH4...

... membrane domains is only partially understood. The secretory-machinery-mediated sorting to ERESs is a process that counters the entropy-driven even distribution of membrane proteins within organellar membranes. Here, for the first time, we quantified the dynamic properties of GFP-VSVG sorting to ERESs...

...Robert G. Parton; Michael Hanzal-Bayer; John F. Hancock Caveolae are striking morphological features of the plasma membrane of mammalian cells. Caveolins, the major proteins of caveolae, play a crucial role in the formation of these invaginations of the plasma membrane; however, the precise...

...Michael J. Allikian; Andrew A. Hack; Stephanie Mewborn; Ulrike Mayer; Elizabeth M. McNally Disruption of the sarcoglycan complex leads to muscle membrane instability and muscular dystrophy in humans and mice. Through the dystrophin glycoprotein complex, sarcoglycan participates in connecting...

...Simon A. Walker; Peter J. Lockyer Ras GTPases are universal molecular switches that act as kinetic timers of signal transduction events. They are post-translationally modified by the addition of lipid groups to their hypervariable carboxyl termini, which plug the proteins to membranes and influence...

...Ursula Rescher; Volker Gerke Annexins are a well-known multigene family of Ca 2+ -regulated phospholipid-binding and membrane-binding proteins. Recent work employing annexin-knockdown or - knockout models has provided new insights into the biological functions of different annexin proteins...

... to the surface of the bacteria-enclosed phagosome. Connecting these abutting membranes are tiny hairs, which are frequently periodic like the rungs of a ladder. These connections are stable and of high affinity - phagosomes from infected macrophages remain connected to the ER and mitochondria (as they were...