... provide a contractility-dependent mechanism by which matrix stiffness and TGFβ1 together mediate EZH2 signaling to promote EMT. Polyacrylamide hydrogels of various stiffnesses were synthesized using an adapted protocol ( Tse and Engler, 2010 ). The hydrogels were composed of an acrylamide monomer (Sigma...

... by controlling cell contractility through a mechanism involving EGFR. Cell contractility Epithelial growth factor receptor Focal adhesion Matrix stiffness Mechanotransduction Tissue transglutaminase National Science Foundation 10.13039/100000001 1740900 National Institutes...

....2017.87 Nasrollahi , S. , Walter , C. , Loza , A. J. , Schimizzi , G. V. , Longmore , G. D. and Pathak , A. ( 2017 ). Past matrix stiffness primes epithelial cells and regulates their future collective migration through a mechanical memory . Biomaterials   146 , 146...

... replacement and crosslinking. Basement membrane Dextran sodium sulfate Drosophila Matrix stiffness Midgut Enterocytes Collagen IV National Institutes of Health R21AR072510 R35GM125028 F31HL136081 R01DK103831 R01DK116964 Burroughs Wellcome Fund 10.13039/100000861...

...://www.biologists.com/user-licence-1-1/ Summary: An interaction between vinculin and an amphipathic helix of vinexin α is required for a conformational change in vinculin and promotion of YAP/TAZ nuclear localization on rigid ECM. Vinexin Vinculin Focal adhesion Matrix stiffness Mechanotransduction...

...Justin D. Mih; Aleksandar Marinkovic; Fei Liu; Asma S. Sharif; Daniel J. Tschumperlin Summary The stiffness of the extracellular matrix exerts powerful effects on cell proliferation and differentiation, but the mechanisms transducing matrix stiffness into cellular fate decisions remain poorly...

... and promoted uniform proliferation on soft gels ( Fig. 1C ). We asked whether the effect of E-cadherin knockdown on the proliferation phenotype is accompanied by changes in cell spreading or occurs independently of cell shape changes. Fig. 3. Matrix stiffness affects the molecular organization...

... stiffness, and examines the relationship between studies in vitro on flat substrates and the more complex three-dimensional setting in vivo. * Author for correspondence ( [email protected] ) © 2011. 2011 Cell mechanics Matrix stiffness Mechanosensing If we probed our environment...

... (ROI) is boxed in the rosette band. (B) After photobleaching the recovery of fluorescence in the ROI was measured. Bars, 10 μm. (C) Experimental data with an exponential recovery. The identified characteristic time is 17 seconds. Podosomes Matrix stiffness Self-organisation Optical flow...