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Keywords: Mass spectrometry
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Journal Articles
J Cell Sci (2022) 135 (16): jcs259993.
Published: 26 August 2022
..., which cortical components are important remains unclear. In this article, we compared cortices from cells with low and high cortex tensions. We purified cortex-enriched fractions from cells in interphase and mitosis, as mitosis is characterised by high cortical tension. Mass spectrometry analysis...
Includes: Supplementary data
Journal Articles
In collection:
Cytoskeleton
J Cell Sci (2022) 135 (9): jcs259108.
Published: 6 May 2022
.... , Zhang , G. , Huang , F.-K. and Neubert , T. A. ( 2015 ). Identification of protein N-termini using TMPP or dimethyl labeling and mass spectrometry . In Proteomic Profiling: Methods and Protocols (ed. A. Posch ), pp. 249 - 258 . New York, NY : Springer New York . Dubey...
Includes: Supplementary data
Journal Articles
J Cell Sci (2021) 134 (15): jcs258484.
Published: 5 August 2021
... microscopy Analytical ultracentrifugation Mass spectrometry In vitro reconstitution Protein biochemistry Agence Nationale de la Recherche http://dx.doi.org/10.13039/501100001665 ANR-17-CE13-0014 Fondation ARC pour la recherche sur le cancer http://dx.doi.org/10.13039...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (24): jcs248864.
Published: 23 December 2020
... determinants that specify the formation and function of these cells are not defined. Here, using tandem mass tag-mass spectrometry, we identified a differentially upregulated protein, C-type lectin domain family 10 member (herein denoted CD301, also known as CLEC10A), that was strongly upregulated in mouse...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (3): jcs238659.
Published: 12 February 2020
... Evolutionary cell biology Mass spectrometry Paralogous expansion Backfilling National Institutes of Health 10.13039/100000002 GM105783 Wellcome Trust 10.13039/100010269 204697/Z/16/Z Eukaryotic cells contain multiple membrane-bound compartments, with either an endogenous...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (24): 5725–5734.
Published: 15 December 2013
... of these complexes are poorly understood. Using immunoprecipitation and mass spectrometry, 4.1G was identified as a new interacting partner for the cyclic-nucleotide gated (CNG) channels in ROSs. 4.1G is a widely expressed multifunctional protein that plays a role in the assembly and stability of membrane protein...
Journal Articles
J Cell Sci (2013) 126 (11): 2480–2492.
Published: 1 June 2013
... ( Müller et al., 2011b ; Müller et al., 2011a ; Spitzer et al., 2010 ). Using the LTQ Orbitrap Velos mass spectrometry instrument, we identified 10,819 proteins in total. Quantification of these proteins was performed using the label-free spectral count based approach ( Müller et al., 2011b ; Müller et...
Includes: Supplementary data
Journal Articles
J Cell Sci (2009) 122 (14): 2424–2435.
Published: 15 July 2009
...Timothy M. E. Scales; Shen Lin; Michaela Kraus; Robert G. Goold; Phillip R. Gordon-Weeks MAP1B is a developmentally regulated microtubule-associated phosphoprotein that regulates microtubule dynamics in growing axons and growth cones. We used mass spectrometry to map 28 phosphorylation sites...
Journal Articles
J Cell Sci (2008) 121 (15): 2565–2577.
Published: 1 August 2008
.... Table 2. Retrogradely transported proteins identified by 2-DE and mass spectrometry Spot number Identified protein GenBank accession number Mascot score Peptides matched Sequence coverage Molecular size (kDa) pI 1 Not identified 2 SH3 domain...
Includes: Supplementary data
Journal Articles
J Cell Sci (2005) 118 (23): 5661–5673.
Published: 1 December 2005
... polarity Mass spectrometry Tandem affinity purification The LKB1 protein kinase was originally identified as a gene mutated in the inherited Peutz Jeghers Syndrome (PJS), in which subjects are predisposed to developing benign and malignant tumours ( Hemminki et al., 1998 ; Jenne et al., 1998...
Includes: Supplementary data
Journal Articles
J Cell Sci (2005) 118 (17): 4009–4016.
Published: 1 September 2005
... 2005 β1 integrin 12G10 Glycosylation Monomer Mass spectrometry Integrins constitute a large family of proteins composed of noncovalently associated α and β heterodimers which play a role in cell signalling and adhesion ( Giancotti, 2003 ; Humphries et al., 2003 ; Hynes, 2002...
Journal Articles
In collection:
Lipid Biology
J Cell Sci (2005) 118 (12): 2601–2611.
Published: 15 June 2005
... contributed equally to this work 17 3 2005 © The Company of Biologists Limited 2005 2005 Lipid droplet Mass spectrometry Rab18 Endoplasmic reticulum Membrane apposition Lipid droplets (LDs) are found in many kinds of cells. Their surface is composed of a phospholipid monolayer...