... Branching morphogenesis Epithelial development Mammary gland TGF-β National Institutes of Health http://dx.doi.org/10.13039/100000002 NIGMS 3T32GM007309 U54CA268083 National Science Foundation http://dx.doi.org/10.13039/100000001 1915491 Breast Cancer Research...

.... These results highlight the importance of the concerted action of extracellular syntaxin4 extruded in response to the lactogenic hormone and BM components in epithelial morphogenesis. Fig. 1. Effect of syntaxin4 on 3D morphogenesis of mammary gland epithelia. (A) Left upper: schematic diagrams of a tip...

...-specific Piezo1 deletion. These findings support a multifaceted system of chemical and mechanical sensing in the mammary gland, and a protective redundancy that ensures continued lactational competence and offspring survival. References Árnadóttir , J. and Chalfie , M. ( 2010...

... in vivo . The adult mammary gland provides a unique model for investigating physiological or pathological processes such as morphogenesis or epithelial cell dissemination. Here, we establish a new pipeline to study rare cellular events occurring in the mammary gland, by combining orthotopic...

.... ( 1997 ). Three division-competent, structurally-distinct cell populations contribute to murine mammary epithelial renewal . Tissue Cell   29 , 239 - 253 . Chepko   G. , Smith   G. H. ( 1999 ). Mammary epithelial stem cells: our current understanding . J. Mammary Gland Biol. Neoplasia...

... family of CLD binding proteins, is hypothesized to regulate CLD production in these cells during differentiation of the mammary gland into a secretory organ. We tested this hypothesis by comparing CLD accumulation in differentiating mammary glands of wild-type and ADPH-deficient mice. ADPH deficiency did...

... on sections cut from PBS-buffered formalin-fixed and paraffin-embedded inguinal mammary glands. In brief, after de-waxing in xylene, antigen retrieval was performed using 1 mM EDTA, pH 9.0 in a pressure cooker. The sections were blocked for 30 minutes in Image-iT FX Signal Enhancer solution (Invitrogen...

...Stephanie B. Greene; Preethi H. Gunaratne; Scott M. Hammond; Jeffrey M. Rosen In an effort to understand the potential role of microRNAs (miRNAs) in mammary-gland stem or progenitor cells, miRNA microarrays were performed on subpopulations of the mouse mammary epithelial cell (MEC) line COMMA-DβGeo...

... Mammary gland Stem Progenitor Cell line COMMA-D β-geo Sca1 Breast epithelium is capable of completely and functionally regenerating upon transplantation. This impressive renewal capacity has been ascribed to the function of a multipotent mammary gland stem/progenitor cell population...

...Mustapha Lkhider; Roberta Castino; Edwige Bouguyon; Ciro Isidoro; Michèle Ollivier-Bousquet The 16 kDa prolactin fragment arises from partial proteolysis of the native 23 kDa prolactin pituitary hormone. The mammary gland has been involved in this processing, although it has not been clarified...

...Stacey B. Tepera; Pierre D. McCrea; Jeffrey M. Rosen The Wnt (wingless) family of secreted glycoproteins initiates a signalling pathway implicated in the regulation of both normal mouse mammary gland development and tumorigenesis. Multiple Wnt signals ultimately converge on the multifunctional...

...-binding proteins (IGFBPs),there is currently no experimental evidence that IGFBPs regulate the survival function of IGFs in the mammary gland. We have examined IGFBP expression during mammary gland development and studied the effects of IGFBPs on IGF-mediated survival and signalling in mammary epithelial...

... inhibitor BB94,indicating that this morphogenetic process is likely to require MMP activity. Collectively, our results provide evidence that RA promotes lumen formation by mammary epithelial cells in vitro and suggest that it plays a similar role during mammary gland development in vivo. Fig. 5. When...

... gland. To investigate its role in the biology of the mammary gland, we have established transgenic mice bearing the EphB4 receptor under the control of the MMTV-LTR promoter, which represents the first transgenic mouse model to investigate the effect(s) of unscheduled expression of EphB4 in adult...

... function in mammary tumors and mammary glands is unknown. To begin to resolve these issues, we analysed in detail the normal postnatal expression patterns and regulation of the CD44v6 epitope in murine mammary glands. We demonstrate that significant CD44v6 epitope expression is first seen during puberty...

...Stuart Naylor; Matthew J. Smalley; David Robertson; Barry A. Gusterson; Paul A. W. Edwards; Trevor C. Dale ABSTRACT Several Wnt genes are expressed in the postnatal mouse mammary gland and are thought to be involved in mammary gland development. Ectopic expression of Wnt-1, which is not normally...

... expression Epithelial-mesenchymal interaction Mammary gland Tissue-specific gene expression in epithelial cells is achieved through integration of multiple and sustained signals from the cellular microenvironment. Mouse mammary epithelial cells isolated from mid-pregnant mammary glands form acinus...

...P. Schedin; R. Strange; T. Mitrenga; P. Wolfe; M. Kaeck ABSTRACT Mammary gland form and function are regulated by interactions between epithelium and extracellular matrix. Major glycoprotein components of extracellular matrix have been identified that give survival, proliferation...

... of goats bearing alleles associated with a reduced α S1 -Cn content in milk affected the Cns but not the main whey proteins. Fig. 2. Immunofluorescence localisation of lactoproteins in mammary acini of goat homozygous for distinct α S1 -Cn alleles. Fragments of the mammary gland of α S1 -CnA...

...Anthony D. Metcalfe; Andrew Gilmore; Teresa Klinowska; Janine Oliver; Anthony J. Valentijn; Robin Brown; Andrea Ross; Grant MacGregor; John A. Hickman; Charles H. Streuli ABSTRACT Epithelial cells within the mammary gland undergo developmental programmes of proliferation and apoptosis during...