... Summary: A small p53 isoform responds specifically laminin-111, a key extracellular matrix component, and regulates the expression of extracellular matrix molecules required to establish correct form and function. Δ133p53 ECM Fibronectin Laminin Mammary epithelial cells p53 3D culture Tissue...

... phenotype relative to cells transformed by the predominant transforming variant of PIK3CA , p110α H1047R . We show here that MCF-10A human mammary epithelial cells expressing wild-type p110β have a phenotype that is distinct from MCF-10A cells transformed due to expression of p110α H1047R . Expression...

...Ewa Grudzien-Nogalska; Brent C. Reed; Robert E. Rhoads ABSTRACT Downregulation of CPEB1, a sequence-specific RNA-binding protein, in a mouse mammary epithelial cell line (CID-9) causes epithelial-to-mesenchymal transition (EMT), based on several criteria. First, CPEB1 knockdown decreases protein...

... Apoptosis resistance Mammary epithelial cells Apoptosis is essential for tissue development and homeostasis and is required for efficient tumor therapy ( Fernandez et al., 2002 ; Igney and Krammer, 2002 ; Zakeri and Lockshin, 2002 ). Aberrant regulation of cell death contributes...

... signaling pathways, and TIMP3 functions to alter β-catenin signaling. Here we demonstrate that primary mouse embryonic fibroblasts (MEFs) and mammary epithelial cells (MECs) deficient in Timp3 have increased β-catenin signaling. Functionally, the loss of TIMP3 exerted cell-type-specific effects, with Timp3...

... the senescence response is antagonistically pleiotropic. We show that premalignant mammary epithelial cells exposed to senescent human fibroblasts in mice irreversibly lose differentiated properties, become invasive and undergo full malignant transformation. Moreover, using cultured mouse or human fibroblasts...

...Monique Rijnkels; Jeffrey M. Rosen The transplantation of primary mammary epithelial cells after adenovirus-Cre-mediated recombination provides a new approach for the study of specific gene function during mammary gland development and in breast cancer. Most mammary-gland-specific promoters...

.... To investigate the biological role of Wnt-5a in the human mammary epithelial cell line HB2, we used an antisense approach to repress endogenous expression of Wnt-5a protein. We also generated a cell population that constitutively overexpresses this protein. We found that overexpression of Wnt-5a protein enhanced...

...Michael A. Matthay; Jean-Paul Thiery; Frank Lafont; Martha F. Stampfer; Brigitte Boyer ABSTRACT The effects of growth factors on epithelial cell motility and dispersion have been examined on an immortalized human mammary epithelial cell line, the 184A1 non-tumorigenic cell line. Among all...