.... Cells were trypsinised, replated with wild-type MDCK cells and grown for 16 h. For Cle-cad and TEVp experiments, ≥2×10 5  cells/cm 2 were seeded onto Transwell ® inserts (12-well, polyester membranes, 0.4-µm pore) for 36 h. Purified TEVp at ∼200 µg/ml (a kind gift from Madelon Maurice, University...

.... This suggests that both polarized targeting and postendocytic trafficking of endolyn are essential for the protein's proper function in mammalian kidney. * Author for correspondence ( weisz@pitt.edu ) 6 8 2012 © 2012. Published by The Company of Biologists Ltd 2012 MDCK Apical Kidney...

...Samuel C. Wolff; Ai-Dong Qi; T. Kendall Harden; Robert A. Nicholas The P2Y 1 receptor is localized to the basolateral membrane of polarized Madin-Darby canine kidney (MDCK) cells. In the present study, we identified a 25-residue region within the C-terminal tail (C-tail) of the P2Y 1 receptor...

... or stabilising cell-cell interactions. In non-ciliated L929 cells, stable or transient surface expression of the PC1 extracellular domain was sufficient to confer an adhesive phenotype and stimulate junction formation. In MDCK cells, we found that PC1 was recruited to the lateral membranes coincident with E...

... regulation in epithelial cells. We used HA-tagged SGK1 to transiently transfect MDCK cells and study the regulation of SGK1 upon stimulation with HGF, cAMP or upon adhesion of the cells to immobilized fibronectin. In addition, we studied the regulation of SGK1 activity by small GTP-binding proteins...