... polarities of the homologous copper-ATPases ATP7A and ATP7B at the trans-Golgi network. ATP7A ATP7B Copper-ATPases Apico-basolateral polarity Adaptor protein-1 MDCK Wellcome Trust DBT India Alliance http://dx.doi.org/10.13039/501100009053 IA/I/16/1/502369 Department...

... was one quarter of the bead volume. For E-cad depletion, cells were transfected with GFP and E-cad-specific shRNA-expressing plasmid and grown for 7 days. Cells were trypsinised, replated with wild-type MDCK cells and grown for 16 h. For Cle-cad and TEVp experiments, ≥2×10 5  cells/cm 2 were...

... suggest that efficient apical delivery and endocytosis are both required for proper endolyn function in the pronephric kidney. * Author for correspondence ( [email protected] ) 6 8 2012 © 2012. Published by The Company of Biologists Ltd 2012 MDCK Apical Kidney development...

...Samuel C. Wolff; Ai-Dong Qi; T. Kendall Harden; Robert A. Nicholas The P2Y 1 receptor is localized to the basolateral membrane of polarized Madin-Darby canine kidney (MDCK) cells. In the present study, we identified a 25-residue region within the C-terminal tail (C-tail) of the P2Y 1 receptor...

... or stabilising cell-cell interactions. In non-ciliated L929 cells, stable or transient surface expression of the PC1 extracellular domain was sufficient to confer an adhesive phenotype and stimulate junction formation. In MDCK cells, we found that PC1 was recruited to the lateral membranes coincident with E...

... regulation in epithelial cells. We used HA-tagged SGK1 to transiently transfect MDCK cells and study the regulation of SGK1 upon stimulation with HGF, cAMP or upon adhesion of the cells to immobilized fibronectin. In addition, we studied the regulation of SGK1 activity by small GTP-binding proteins...

... therefore suggest that vinculin phosphorylation by protein kinase C is a crucial step in the correct assembly of the epithelial junctional complex. Fig. 2. Western blot and autoradiographic detection of vinculin and α-actinin immunoprecipitates from metabolically labeled MDCK cells under different...

... surface is unclear. To investigate the role of β1 integrins, the major receptor family for collagen, in this process, we overlaid polarized monolayers of MDCK II cells grown on permeable supports with type I collagen gel and correlated integrin polarity with the polarity of other apical and basolateral...

...Sandra Citi; Natasha Denisenko ABSTRACT In previous studies we have shown that protein kinase inhibitors and extracellular calcium can affect dramatically the assembly of tight junctions (TJ) and the localization of the TJ protein cingulin at sites of cell-cell contact in renal epithelial (MDCK...

.... In polarized MDCK cells, EGE’ was localized almost exclu-sively to the apical cell surface, while in polarized intesti-nal Caco-2 cells, although 80% of the extracellular form of the enzyme was routed through the apical membrane over a 24 hour period, EGE’ was also detected at the basolateral membrane. Rates...

... of receptors. To begin to understand the role of integrins in polarization, we have investigated the expression and localization of three integrin families in the polarized Madin-Darby canine kidney (MDCK) epithelial cell line and in transformed MDCK cells lacking apical polarity. We find that MDCK cells...

... cells and epithelial MDCK cells. Cells were oriented and elongated by these surfaces. Their responsiveness was dependent on cell type, cell-cell interactions, and the geometry of the patterns. Alignment of BHK cells increased with increasing pattern period. Single MDCK cells aligned to all pattern...

...Kathleen L. Soole; Judith Hall; Mark A. Jepson; Geoffrey P. Hazlewood; Harry J. Gilbert; Barry H. Hirst ABSTRACT The constitutive (or default) pathway for protein secretion was investigated in two epithelial cells, Madin-Darby canine kidney (MDCK) and human colonic adenocarcinoma (Caco-2), using...